Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa

Detalhes bibliográficos
Autor(a) principal: Santos, Patrícia Nunes dos
Data de Publicação: 2020
Outros Autores: Paz, Francisco Adalberto do Nascimento, Santos, Elanne Nunes dos, Batista, Nelson Jorge Carvalho, Carvalho, Tássio Mascarenhas de, Costa, Charllyton Luiz Sena da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/2968
Resumo: Consumption of products and substances of plant origin is common practice in populations all over the planet. Morus nigra L. (blackberry) is a medicinal plant widely used in folk medicine, and has several active ingredients with therapeutic action, such as antioxidant, antinoceptive, hypoglycemic and anti-inflammatory activity. The aim of the present study was to analyze the possible cytotoxic, genotoxic and mutagenic activities of Morus nigra L. leaves through the Allium cepa test. For preparation of the extract were used blackberry leaves, collected in the city of Agricolândia-PI. Three different concentrations (0.007 mg / mL, 0.07 mg / mL and 0.7 mg / mL) were used, in addition to the negative and positive controls. For each extract concentration, 10 onion bulbs were used, which were exposed for germination. After growth, the roots were measured with the help of a ruler, cut and followed by the slide preparation procedure, which were visualized under a light microscope at 100x magnification and analyzed qualitatively for the presence of genetic damage. Statistical analysis was performed using the ANOVA system of mean sample results. After data analysis, it was observed that the size of root meristems indicated no toxicity, the mitotic indexes of A. cepa cells exposed at these concentrations were not statistically different from the negative control. The chromosomal aberrations found were not significant (P <0.05) in the concentrations used and micronuclei and binucleated cells did not show significant variations when compared to the negative control results. Thus, based on the results, the use of Morus nigra leaves is safe at the concentrations studied, but some studies can be performed to confirm the results and suggest greater reliability in the use of this plant by the population safely.
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spelling Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepaAnálisis del potencial citotóxico, genotóxico y mutagénico del extracto hidroalcohólico de hojas de Morus nigra L. Através del bioensayo Allium cepaAnálise do potencial citotóxico, genotóxico e mutagênico do extrato hidroalcóolico das folhas da Morus nigra L. Através do bioensaio Allium cepaMorus nigra L. Planta. Allium cepaMorus nigra L. Planta. Allium cepaMorus nigra LPlantAllium strainConsumption of products and substances of plant origin is common practice in populations all over the planet. Morus nigra L. (blackberry) is a medicinal plant widely used in folk medicine, and has several active ingredients with therapeutic action, such as antioxidant, antinoceptive, hypoglycemic and anti-inflammatory activity. The aim of the present study was to analyze the possible cytotoxic, genotoxic and mutagenic activities of Morus nigra L. leaves through the Allium cepa test. For preparation of the extract were used blackberry leaves, collected in the city of Agricolândia-PI. Three different concentrations (0.007 mg / mL, 0.07 mg / mL and 0.7 mg / mL) were used, in addition to the negative and positive controls. For each extract concentration, 10 onion bulbs were used, which were exposed for germination. After growth, the roots were measured with the help of a ruler, cut and followed by the slide preparation procedure, which were visualized under a light microscope at 100x magnification and analyzed qualitatively for the presence of genetic damage. Statistical analysis was performed using the ANOVA system of mean sample results. After data analysis, it was observed that the size of root meristems indicated no toxicity, the mitotic indexes of A. cepa cells exposed at these concentrations were not statistically different from the negative control. The chromosomal aberrations found were not significant (P <0.05) in the concentrations used and micronuclei and binucleated cells did not show significant variations when compared to the negative control results. Thus, based on the results, the use of Morus nigra leaves is safe at the concentrations studied, but some studies can be performed to confirm the results and suggest greater reliability in the use of this plant by the population safely.El consumo de productos y sustancias de origen vegetal es una práctica común en poblaciones de todo el planeta. Morus nigra L. (mora) es una planta medicinal ampliamente utilizada en medicina popular, y tiene varios ingredientes activos con acción terapéutica, como actividad antioxidante, antinoceptiva, hipoglucémica y antiinflamatoria. El objetivo del presente estudio fue analizar las posibles actividades citotóxicas, genotóxicas y mutagénicas de las hojas de Morus nigra L. a través de la prueba Allium cepa. Para la preparación del extracto se utilizaron hojas de mora, recolectadas en la ciudad de Agricolândia-PI. Se utilizaron tres concentraciones diferentes (0,007 mg / ml, 0,07 mg / ml y 0,7 mg / ml), además de los controles negativos y positivos. Para cada concentración de extracto, se utilizaron 10 bulbos de cebolla, que fueron expuestos para la germinación. Después del crecimiento, las raíces se midieron con la ayuda de una regla, se cortaron y luego se realizó el procedimiento de preparación del portaobjetos, que se visualizó bajo un microscopio óptico con un aumento de 100x y se analizó cualitativamente la presencia de daño genético. El análisis estadístico se realizó utilizando el sistema ANOVA de resultados de muestras medias. Después del análisis de los datos, se observó que el tamaño de los meristemos de la raíz no indicaba toxicidad, los índices mitóticos de las células de A. cepa expuestas a estas concentraciones no eran estadísticamente diferentes del control negativo. Las aberraciones cromosómicas encontradas no fueron significativas (P <0.05) en las concentraciones utilizadas y los micronúcleos y las células binucleadas no mostraron variaciones significativas en comparación con los resultados del control negativo. Por lo tanto, según los resultados, el uso de hojas de Morus nigra es seguro a las concentraciones estudiadas, pero se pueden realizar algunos estudios para confirmar los resultados y sugerir una mayor confiabilidad en el uso de esta planta por parte de la población de manera segura.O consumo de produtos e substâncias de origem vegetal é prática comum em ameaças de todo o planeta. Um Morus negro (amoreira-preta) é uma planta medicinal bastante utilizada na medicina popular e possui vários princípios com ação terapêutica, como por exemplo, atividade antioxidante, antinoceptiva, hipoglicemiante e anti-inflamatória. O Objective do Presente Estudo foi analisar como Possíveis Atividades citotóxicas, genotóxicas e mutagênicas das Folhas da Morus nigra L. atraves do teste Allium cepa. Para preparar o extrato foram utilizados folhas de amora, coletadas na cidade de Agricolândia-PI. Foram usados três tipos diferentes (0,007 mg / mL, 0,07 mg / mL e 0,7 mg / mL), além do controle negativo e positivo. Para cada concentração de extrato, são utilizados 10 bulbos de cebola, que ficam expostos para germinação. Após o crescimento como raízes foram medidas com auxílio de régua, cortadas e seguidas, ou procedimento de preparação de lâminas, que foram visualizadas no microscópio óptico em um aumento de 100x e analisadas qualificativamente relativamente à presença de danos genéticos. Uma análise estatística foi feita através do sistema ANOVA da média dos resultados das amostras. Após análise dos dados, foi observado que, o tamanho dos meristemas de raízes indica ausência de toxicidade, os índices mitóticos das células deA. cepa também não foi estatisticamente diferente do controle negativo. Como as aberturas cromossômicas encontradas não foram usadas (P <0,05) nas aplicações usadas e micronúcleos e células binucleadas que não são aplicadas quando você usa os resultados negativos de controle negativo. Desta forma, com base nos resultados ou uso das folhas do Morus mostra-se seguro em estudos, porém alguns estudos podem ser executados sem sentido de verificação dos resultados e sugerir maiores usos sem uso dessa planta pela população de forma segura .Research, Society and Development2020-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/296810.33448/rsd-v9i4.2968Research, Society and Development; Vol. 9 No. 4; e132942968Research, Society and Development; Vol. 9 Núm. 4; e132942968Research, Society and Development; v. 9 n. 4; e1329429682525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/2968/2317Copyright (c) 2020 PATRICIA NUNES DOS SANTOSinfo:eu-repo/semantics/openAccessSantos, Patrícia Nunes dosPaz, Francisco Adalberto do NascimentoSantos, Elanne Nunes dosBatista, Nelson Jorge CarvalhoCarvalho, Tássio Mascarenhas deCosta, Charllyton Luiz Sena da2020-08-20T18:07:16Zoai:ojs.pkp.sfu.ca:article/2968Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:27:20.528759Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
Análisis del potencial citotóxico, genotóxico y mutagénico del extracto hidroalcohólico de hojas de Morus nigra L. Através del bioensayo Allium cepa
Análise do potencial citotóxico, genotóxico e mutagênico do extrato hidroalcóolico das folhas da Morus nigra L. Através do bioensaio Allium cepa
title Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
spellingShingle Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
Santos, Patrícia Nunes dos
Morus nigra L. Planta. Allium cepa
Morus nigra L. Planta. Allium cepa
Morus nigra L
Plant
Allium strain
title_short Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
title_full Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
title_fullStr Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
title_full_unstemmed Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
title_sort Analysis of the cytotoxic, genotoxic and mutagenic potential of the hydroalcoholic extract of Morus nigra L. Leaves through the bioassay Allium cepa
author Santos, Patrícia Nunes dos
author_facet Santos, Patrícia Nunes dos
Paz, Francisco Adalberto do Nascimento
Santos, Elanne Nunes dos
Batista, Nelson Jorge Carvalho
Carvalho, Tássio Mascarenhas de
Costa, Charllyton Luiz Sena da
author_role author
author2 Paz, Francisco Adalberto do Nascimento
Santos, Elanne Nunes dos
Batista, Nelson Jorge Carvalho
Carvalho, Tássio Mascarenhas de
Costa, Charllyton Luiz Sena da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Patrícia Nunes dos
Paz, Francisco Adalberto do Nascimento
Santos, Elanne Nunes dos
Batista, Nelson Jorge Carvalho
Carvalho, Tássio Mascarenhas de
Costa, Charllyton Luiz Sena da
dc.subject.por.fl_str_mv Morus nigra L. Planta. Allium cepa
Morus nigra L. Planta. Allium cepa
Morus nigra L
Plant
Allium strain
topic Morus nigra L. Planta. Allium cepa
Morus nigra L. Planta. Allium cepa
Morus nigra L
Plant
Allium strain
description Consumption of products and substances of plant origin is common practice in populations all over the planet. Morus nigra L. (blackberry) is a medicinal plant widely used in folk medicine, and has several active ingredients with therapeutic action, such as antioxidant, antinoceptive, hypoglycemic and anti-inflammatory activity. The aim of the present study was to analyze the possible cytotoxic, genotoxic and mutagenic activities of Morus nigra L. leaves through the Allium cepa test. For preparation of the extract were used blackberry leaves, collected in the city of Agricolândia-PI. Three different concentrations (0.007 mg / mL, 0.07 mg / mL and 0.7 mg / mL) were used, in addition to the negative and positive controls. For each extract concentration, 10 onion bulbs were used, which were exposed for germination. After growth, the roots were measured with the help of a ruler, cut and followed by the slide preparation procedure, which were visualized under a light microscope at 100x magnification and analyzed qualitatively for the presence of genetic damage. Statistical analysis was performed using the ANOVA system of mean sample results. After data analysis, it was observed that the size of root meristems indicated no toxicity, the mitotic indexes of A. cepa cells exposed at these concentrations were not statistically different from the negative control. The chromosomal aberrations found were not significant (P <0.05) in the concentrations used and micronuclei and binucleated cells did not show significant variations when compared to the negative control results. Thus, based on the results, the use of Morus nigra leaves is safe at the concentrations studied, but some studies can be performed to confirm the results and suggest greater reliability in the use of this plant by the population safely.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/2968
10.33448/rsd-v9i4.2968
url https://rsdjournal.org/index.php/rsd/article/view/2968
identifier_str_mv 10.33448/rsd-v9i4.2968
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/2968/2317
dc.rights.driver.fl_str_mv Copyright (c) 2020 PATRICIA NUNES DOS SANTOS
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 PATRICIA NUNES DOS SANTOS
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 4; e132942968
Research, Society and Development; Vol. 9 Núm. 4; e132942968
Research, Society and Development; v. 9 n. 4; e132942968
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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