Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs

Detalhes bibliográficos
Autor(a) principal: Pereira, Douglas Campos
Data de Publicação: 2021
Outros Autores: Mirabal, Bernardo, França, Adriele Maria Machado de, Antonio, Edma Santos de, Fraga, Ricardo Evangelista, Tomazi, Laize
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/19330
Resumo: Blue and yellow macaw is a species which does not show sexual dimorphism and is threatened by animal traffic. The identification of heterosexual pairs is important for reintroduction programs. The aim of this work was select parameters for sexing and use them to determine the frequency of heterosexual pairs in a population of blue-and-yellow macaws allocated in a Wild Animal Screening Center. Blood samples from 23 macaws were collected and genomic DNA extracted by Tris/SDS washes. Allele-specific molecular markers for sexing were amplified by PCR, and identified on 2% agarose gel. Three pairs of primers were tested: Pair 1 (P2/P8), Pair 2 (1237L/1272H) and Pair 3 (2550F/2718R). For the determination of animal pairs, all individuals had their social behavioral acts observed. The results showed that the low complexity DNA extraction protocol used was adequate. Pairs 2 and 3 of primers were effective for sexing and the Pair 3 was the most efficient. The study also showed that in the sample studied, the composition of males and females was similar (0.4 males n=10 and 0.6 females n=13); 70% (n=16) of the individuals formed pairs and 75% (n=12) of the pairs were heterosexual and the others male-male or female-female pairs. These results were used in the management of the animals in the reintroduction program.
id UNIFEI_e43dfe355645f147df444ddb7319ee3b
oai_identifier_str oai:ojs.pkp.sfu.ca:article/19330
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programsSexado molecular en la formación de parejas de guacamayo azul y amarillo (Ara ararauna) en programas de reintroducciónSexagem molecular na formação de pares de arara-canindé (Ara ararauna) em programas de reintroduçãoProgramas de reintroducciónTráfico ilegalMarcadores molecularesPsittacidaeParejas heterosexuales y homosexuales.Programas de reintroduçãoComércio ilegalMarcadores molecularesPsittacidaePares heterossexuais e homossexuais.Reintroduction programsIllegal tradeMolecular markersPsittacidaeHeterosexual and homosexual pairs.Blue and yellow macaw is a species which does not show sexual dimorphism and is threatened by animal traffic. The identification of heterosexual pairs is important for reintroduction programs. The aim of this work was select parameters for sexing and use them to determine the frequency of heterosexual pairs in a population of blue-and-yellow macaws allocated in a Wild Animal Screening Center. Blood samples from 23 macaws were collected and genomic DNA extracted by Tris/SDS washes. Allele-specific molecular markers for sexing were amplified by PCR, and identified on 2% agarose gel. Three pairs of primers were tested: Pair 1 (P2/P8), Pair 2 (1237L/1272H) and Pair 3 (2550F/2718R). For the determination of animal pairs, all individuals had their social behavioral acts observed. The results showed that the low complexity DNA extraction protocol used was adequate. Pairs 2 and 3 of primers were effective for sexing and the Pair 3 was the most efficient. The study also showed that in the sample studied, the composition of males and females was similar (0.4 males n=10 and 0.6 females n=13); 70% (n=16) of the individuals formed pairs and 75% (n=12) of the pairs were heterosexual and the others male-male or female-female pairs. These results were used in the management of the animals in the reintroduction program.Guacamayo azul y amarillo, ave que no presenta dimorfismo sexual, es una especie amenazada por el tráfico de animales. La identificación de parejas heterosexuales es importante para los programas de reintroducción. El objetivo de este trabajo fue seleccionar parámetros para sexar y utilizarlos para determinar la frecuencia de parejas heterosexuales en una población de guacamayos azules y amarillos asignados en un Centro de Detección de Animales Salvajes. Se recolectaron muestras de sangre de 23 guacamayo azul y amarillo y se extrajo el ADN genómico mediante lavados con Tris/SDS. Los marcadores moleculares alelo-específicos para sexar se amplificaron mediante PCR y se identificaron en gel de agarosa al 2%. Se probaron tres pares de cebadores: Par 1 (P2/P8), Par 2 (1237L/1272H) y Par 3 (2550F/2718R). Para la determinación de las parejas de animales, se observaron los comportamientos sociales de todos los individuos. Los resultados mostraron que el protocolo de extracción de ADN de baja complejidad utilizado fue adecuado. Los pares 2 y 3 de cebadores fueron efectivos para sexar y el par 3 fue el más eficiente. El estudio también mostró que en la muestra estudiada, la composición de hombres y mujeres fue similar (0.4 hombres n=10 y 0.6 mujeres n=13); 70% (n=16) de los individuos formaban parejas, y 75% (n=12) de las parejas eran heterosexuales y el resto de parejas hombre-hombre o mujer-mujer. Estos resultados se utilizaron en el manejo de los animales en el programa de reintroducción.Arara-canindé, ave que não apresenta dimorfismo sexual, é uma espécie ameaçada pelo tráfico de animais. A identificação de pares heterossexuais é importante para programas de reintrodução. O objetivo deste trabalho foi selecionar parâmetros de sexagem e utilizá-los para determinar a frequência de pares heterossexuais em uma população de araras-canindé alocada em um Centro de Triagem de Animais Silvestres. Amostras de sangue de 23 araras foram coletadas e o DNA genômico extraído por lavagens com Tris/SDS. Marcadores moleculares alelo-específicos para sexagem foram amplificados por PCR e identificados em gel de agarose a 2%. Três pares de primers foram testados: Par 1 (P2/P8), Par 2 (1237L/1272H) e Par 3 (2550F/2718R). Para a determinação dos pares, todos os indivíduos tiveram seus comportamentos sociais observados. Os resultados mostraram que o protocolo de extração de DNA de baixa complexidade utilizado foi adequado. Os pares 2 e 3 de primers foram eficazes para a sexagem e o Par 3 foi o mais eficiente. O estudo também mostrou que, na amostra estudada, a composição de machos e fêmeas foi semelhante (0,4 machos n=10 e 0,6 fêmeas n=13); 70% (n=16) dos indivíduos formavam pares e 75% (n=12) dos pares eram heterossexuais e os demais pares macho-macho ou fêmea-fêmea. Esses resultados foram utilizados no manejo dos animais no programa de reintrodução.Research, Society and Development2021-08-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1933010.33448/rsd-v10i10.19330Research, Society and Development; Vol. 10 No. 10; 570101019330Research, Society and Development; Vol. 10 Núm. 10; 570101019330Research, Society and Development; v. 10 n. 10; 5701010193302525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/19330/17159Copyright (c) 2021 Douglas Campos Pereira; Bernardo Mirabal; Adriele Maria Machado de França; Edma Santos de Antonio; Ricardo Evangelista Fraga; Laize Tomazihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPereira, Douglas CamposMirabal, Bernardo França, Adriele Maria Machado deAntonio, Edma Santos deFraga, Ricardo EvangelistaTomazi, Laize2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/19330Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:14.722819Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
Sexado molecular en la formación de parejas de guacamayo azul y amarillo (Ara ararauna) en programas de reintroducción
Sexagem molecular na formação de pares de arara-canindé (Ara ararauna) em programas de reintrodução
title Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
spellingShingle Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
Pereira, Douglas Campos
Programas de reintroducción
Tráfico ilegal
Marcadores moleculares
Psittacidae
Parejas heterosexuales y homosexuales.
Programas de reintrodução
Comércio ilegal
Marcadores moleculares
Psittacidae
Pares heterossexuais e homossexuais.
Reintroduction programs
Illegal trade
Molecular markers
Psittacidae
Heterosexual and homosexual pairs.
title_short Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
title_full Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
title_fullStr Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
title_full_unstemmed Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
title_sort Molecular sexing in the formation of pairs of blue-and-yellow macaw (Ara ararauna) in reintroduction programs
author Pereira, Douglas Campos
author_facet Pereira, Douglas Campos
Mirabal, Bernardo
França, Adriele Maria Machado de
Antonio, Edma Santos de
Fraga, Ricardo Evangelista
Tomazi, Laize
author_role author
author2 Mirabal, Bernardo
França, Adriele Maria Machado de
Antonio, Edma Santos de
Fraga, Ricardo Evangelista
Tomazi, Laize
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Douglas Campos
Mirabal, Bernardo
França, Adriele Maria Machado de
Antonio, Edma Santos de
Fraga, Ricardo Evangelista
Tomazi, Laize
dc.subject.por.fl_str_mv Programas de reintroducción
Tráfico ilegal
Marcadores moleculares
Psittacidae
Parejas heterosexuales y homosexuales.
Programas de reintrodução
Comércio ilegal
Marcadores moleculares
Psittacidae
Pares heterossexuais e homossexuais.
Reintroduction programs
Illegal trade
Molecular markers
Psittacidae
Heterosexual and homosexual pairs.
topic Programas de reintroducción
Tráfico ilegal
Marcadores moleculares
Psittacidae
Parejas heterosexuales y homosexuales.
Programas de reintrodução
Comércio ilegal
Marcadores moleculares
Psittacidae
Pares heterossexuais e homossexuais.
Reintroduction programs
Illegal trade
Molecular markers
Psittacidae
Heterosexual and homosexual pairs.
description Blue and yellow macaw is a species which does not show sexual dimorphism and is threatened by animal traffic. The identification of heterosexual pairs is important for reintroduction programs. The aim of this work was select parameters for sexing and use them to determine the frequency of heterosexual pairs in a population of blue-and-yellow macaws allocated in a Wild Animal Screening Center. Blood samples from 23 macaws were collected and genomic DNA extracted by Tris/SDS washes. Allele-specific molecular markers for sexing were amplified by PCR, and identified on 2% agarose gel. Three pairs of primers were tested: Pair 1 (P2/P8), Pair 2 (1237L/1272H) and Pair 3 (2550F/2718R). For the determination of animal pairs, all individuals had their social behavioral acts observed. The results showed that the low complexity DNA extraction protocol used was adequate. Pairs 2 and 3 of primers were effective for sexing and the Pair 3 was the most efficient. The study also showed that in the sample studied, the composition of males and females was similar (0.4 males n=10 and 0.6 females n=13); 70% (n=16) of the individuals formed pairs and 75% (n=12) of the pairs were heterosexual and the others male-male or female-female pairs. These results were used in the management of the animals in the reintroduction program.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/19330
10.33448/rsd-v10i10.19330
url https://rsdjournal.org/index.php/rsd/article/view/19330
identifier_str_mv 10.33448/rsd-v10i10.19330
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/19330/17159
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 10; 570101019330
Research, Society and Development; Vol. 10 Núm. 10; 570101019330
Research, Society and Development; v. 10 n. 10; 570101019330
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052754375475200

Registros relacionados