Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa

Detalhes bibliográficos
Autor(a) principal: Amado, Paula Avelar
Data de Publicação: 2021
Outros Autores: Castro, Ana Hortência Fonsêca, Lima, Luciana Alves Rodrigues dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/17199
Resumo: Smilax brasiliensis (Smilacaceae) is a native Brazilian plant found in the Cerrado biome and commonly used in folk   medicine. The aim of this study was to evaluate the influence of the methanol extract and fractions from S. brasiliensis leaves on development in vitro of Nicotiana tabacum (tobacco) and Allium cepa (onion) seeds. In vitro germination protocol of onion seeds was established. Tobacco and onion seeds were placed to germinate on basal medium added of extract or fractions of S. brasiliensis leaves and dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and gibberellic acid (GA) at different concentrations in the presence of light. The germination ranged from 40 to 100% for tobacco and from 60 to 100% for onion seeds. The results indicated that the extract and fractions promoted higher growth than 2,4-D and GA when analyzed number of nodes, leaves, root and root size for germinated tobacco seeds, and higher growth when analyzed number roots and stem size for onion seeds. Regarding BAP, the extract and fractions inhibited leaf growth, root and root size of tobacco seeds. Further studies are needed to evaluate the possible use of the methanol extract and fractions from S. brasiliensis leaves as natural sources of hormones and/or bioherbicides.
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spelling Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepaInfluencia del extracto de metanol y fracciones de Smilax brasiliensis Sprengel en el desarrollo in vitro de Nicotiana tabacum y Alllium cepaInfluência do extrato metanólico e das frações de Smilax brasiliensis Sprengel no desenvolvimento in vitro de Nicotiana tabacum e Allium cepaGerminação in vitroAuxinaCitocininaGiberelinaCerrado brasileiro.AuxinCytokininGibberellinIn vitro germinationBrazilian Cerrado.Germinación in vitroAuxinaCitoquininaGiberelinaCerrado brasileño.Smilax brasiliensis (Smilacaceae) is a native Brazilian plant found in the Cerrado biome and commonly used in folk   medicine. The aim of this study was to evaluate the influence of the methanol extract and fractions from S. brasiliensis leaves on development in vitro of Nicotiana tabacum (tobacco) and Allium cepa (onion) seeds. In vitro germination protocol of onion seeds was established. Tobacco and onion seeds were placed to germinate on basal medium added of extract or fractions of S. brasiliensis leaves and dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and gibberellic acid (GA) at different concentrations in the presence of light. The germination ranged from 40 to 100% for tobacco and from 60 to 100% for onion seeds. The results indicated that the extract and fractions promoted higher growth than 2,4-D and GA when analyzed number of nodes, leaves, root and root size for germinated tobacco seeds, and higher growth when analyzed number roots and stem size for onion seeds. Regarding BAP, the extract and fractions inhibited leaf growth, root and root size of tobacco seeds. Further studies are needed to evaluate the possible use of the methanol extract and fractions from S. brasiliensis leaves as natural sources of hormones and/or bioherbicides.Smilax brasiliensis es una planta originaria del Cerrado brasileño y de uso común en la medicina popular. El objetivo de este estudio fue evaluar la influencia del extracto de metanol y fracciones de hojas de S. brasiliensis en el desarrollo in vitro de semillas de Nicotiana tabacum (tabaco) y Allium cepa (cebolla). Se estableció un protocolo de germinación in vitro para semillas de cebolla. Se colocaron semillas de tabaco y cebolla para germinar en medio basal al que se le adicionó extracto o fracciones de hojas de S. brasiliensis y ácido diclorofenoxiacético (2,4-D), 6-bencilaminopurina (BAP) y ácido giberélico (AG) a diferentes concentraciones en presencia de luz. El porcentaje de germinación varió del 40 al 100% para el tabaco y del 60 al 100% para la cebolla. Se colocaron semillas de tabaco y cebolla para germinar en medio basal al que se le adicionó extracto o fracciones de hojas de S. brasiliensis y ácido diclorofenoxiacético (2,4-D), 6-bencilaminopurina (BAP) y ácido giberélico (AG) a diferentes concentraciones en presencia de luz. El porcentaje de germinación varió del 40 al 100% para el tabaco y del 60 al 100% para la cebolla. Los resultados indicaron que el extracto y las fracciones promovieron mayor crecimiento que 2,4-D y AG, al analizar el número de nudos, hojas, tamaño de raíz y raíz para semillas de tabaco germinadas, y mayor crecimiento al analizar número de raíz y tamaño de tallo para cebolla semillas. En relación con BAP, el extracto y las fracciones inhibieron el crecimiento de hojas, raíz y tamaño de raíz de semillas de tabaco germinadas. Se necesitan más estudios para evaluar el posible uso de extracto de metanol y fracciones de hojas de S. brasiliensis como fuentes naturales de hormonas y/o bioherbicidas.Smilax brasiliensis é uma planta nativa do Cerrado brasileiro e comumente usada na medicina popular. O objetivo deste estudo foi avaliar a influência do extrato metanólico e das frações das folhas de S. brasiliensis no desenvolvimento in vitro de sementes de Nicotiana tabacum (tabaco) e Allium cepa (cebola). Foi estabelecido um protocolo de germinação in vitro de sementes de cebola. Sementes de tabaco e cebola foram colocadas para germinar em meio basal adicionado de extrato ou frações das folhas de S. brasiliensis e ácido diclorofenoxiacético (2,4-D), 6-benzilaminopurina (BAP) e ácido giberélico (AG) em diferentes concentrações na presença de luz. A porcentagem de germinação variou de 40 a 100% para o tabaco e de 60 a 100% para a cebola. Os resultados indicaram que o extrato e as frações promoveram maior crescimento do que 2,4-D e AG, quando analisados número de nós, folhas, raiz e tamanho da raiz para sementes de tabaco germinadas, e maior crescimento quando analisado número de raiz e tamanho do caule para sementes de cebola. Em relação ao BAP, o extrato e as frações inibiram o crescimento das folhas, raiz e tamanho da raiz das sementes de tabaco germinadas. Mais estudos são necessários para avaliar a possível utilização do extrato metanólico e frações das folhas de S. brasiliensis como fontes naturais de hormônios e/ou bioherbicidas.Research, Society and Development2021-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1719910.33448/rsd-v10i8.17199Research, Society and Development; Vol. 10 No. 8; e26410817199Research, Society and Development; Vol. 10 Núm. 8; e26410817199Research, Society and Development; v. 10 n. 8; e264108171992525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/17199/15472Copyright (c) 2021 Paula Avelar Amado; Ana Hortência Fonsêca Castro; Luciana Alves Rodrigues dos Santos Limahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAmado, Paula AvelarCastro, Ana Hortência FonsêcaLima, Luciana Alves Rodrigues dos Santos2021-08-21T18:46:59Zoai:ojs.pkp.sfu.ca:article/17199Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:35.297379Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
Influencia del extracto de metanol y fracciones de Smilax brasiliensis Sprengel en el desarrollo in vitro de Nicotiana tabacum y Alllium cepa
Influência do extrato metanólico e das frações de Smilax brasiliensis Sprengel no desenvolvimento in vitro de Nicotiana tabacum e Allium cepa
title Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
spellingShingle Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
Amado, Paula Avelar
Germinação in vitro
Auxina
Citocinina
Giberelina
Cerrado brasileiro.
Auxin
Cytokinin
Gibberellin
In vitro germination
Brazilian Cerrado.
Germinación in vitro
Auxina
Citoquinina
Giberelina
Cerrado brasileño.
title_short Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
title_full Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
title_fullStr Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
title_full_unstemmed Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
title_sort Influence of the methanol extract and fractions of Smilax brasiliensis Sprengel on development in vitro of Nicotiana tabacum and Alllium cepa
author Amado, Paula Avelar
author_facet Amado, Paula Avelar
Castro, Ana Hortência Fonsêca
Lima, Luciana Alves Rodrigues dos Santos
author_role author
author2 Castro, Ana Hortência Fonsêca
Lima, Luciana Alves Rodrigues dos Santos
author2_role author
author
dc.contributor.author.fl_str_mv Amado, Paula Avelar
Castro, Ana Hortência Fonsêca
Lima, Luciana Alves Rodrigues dos Santos
dc.subject.por.fl_str_mv Germinação in vitro
Auxina
Citocinina
Giberelina
Cerrado brasileiro.
Auxin
Cytokinin
Gibberellin
In vitro germination
Brazilian Cerrado.
Germinación in vitro
Auxina
Citoquinina
Giberelina
Cerrado brasileño.
topic Germinação in vitro
Auxina
Citocinina
Giberelina
Cerrado brasileiro.
Auxin
Cytokinin
Gibberellin
In vitro germination
Brazilian Cerrado.
Germinación in vitro
Auxina
Citoquinina
Giberelina
Cerrado brasileño.
description Smilax brasiliensis (Smilacaceae) is a native Brazilian plant found in the Cerrado biome and commonly used in folk   medicine. The aim of this study was to evaluate the influence of the methanol extract and fractions from S. brasiliensis leaves on development in vitro of Nicotiana tabacum (tobacco) and Allium cepa (onion) seeds. In vitro germination protocol of onion seeds was established. Tobacco and onion seeds were placed to germinate on basal medium added of extract or fractions of S. brasiliensis leaves and dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and gibberellic acid (GA) at different concentrations in the presence of light. The germination ranged from 40 to 100% for tobacco and from 60 to 100% for onion seeds. The results indicated that the extract and fractions promoted higher growth than 2,4-D and GA when analyzed number of nodes, leaves, root and root size for germinated tobacco seeds, and higher growth when analyzed number roots and stem size for onion seeds. Regarding BAP, the extract and fractions inhibited leaf growth, root and root size of tobacco seeds. Further studies are needed to evaluate the possible use of the methanol extract and fractions from S. brasiliensis leaves as natural sources of hormones and/or bioherbicides.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17199
10.33448/rsd-v10i8.17199
url https://rsdjournal.org/index.php/rsd/article/view/17199
identifier_str_mv 10.33448/rsd-v10i8.17199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17199/15472
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 8; e26410817199
Research, Society and Development; Vol. 10 Núm. 8; e26410817199
Research, Society and Development; v. 10 n. 8; e26410817199
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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