Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
Main Author: | |
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Publication Date: | 2019 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/s1984-296120180088 http://hdl.handle.net/11449/188934 |
Summary: | Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms. |
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Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast BrazilInfecção de Ehrlichia spp. em gatos domésticos da Grande Rio de Janeiro, sudeste do BrasilEhrlichiosisFelineHematologyIFANested PCREhrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.Departamento de Medicina e Cirurgia Veterinária Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro - UFRRJUniversidade Federal Fluminense - UFFDepartamento de Epidemiologia e Saúde Pública Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro - UFRRJDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPUniversidade Federal Rural do Rio de Janeiro - UFRRJUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Guimarães, AndresaRaimundo, Juliana MacedoRodrigues, Raisa BraulPeixoto, Maristela PeckleSantos, Huarrisson AzevedoAndré, Marcos Rogério [UNESP]Machado, Rosangela Zacarias [UNESP]Baldani, Cristiane Divan2019-10-06T16:23:55Z2019-10-06T16:23:55Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-185application/pdfhttp://dx.doi.org/10.1590/s1984-296120180088Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019.0103-846Xhttp://hdl.handle.net/11449/18893410.1590/s1984-296120180088S1984-296120190001001802-s2.0-85063923138S1984-29612019000100180.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:48Zoai:repositorio.unesp.br:11449/188934Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:07:47.389695Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil Infecção de Ehrlichia spp. em gatos domésticos da Grande Rio de Janeiro, sudeste do Brasil |
title |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
spellingShingle |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil Guimarães, Andresa Ehrlichiosis Feline Hematology IFA Nested PCR |
title_short |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
title_full |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
title_fullStr |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
title_full_unstemmed |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
title_sort |
Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
author |
Guimarães, Andresa |
author_facet |
Guimarães, Andresa Raimundo, Juliana Macedo Rodrigues, Raisa Braul Peixoto, Maristela Peckle Santos, Huarrisson Azevedo André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] Baldani, Cristiane Divan |
author_role |
author |
author2 |
Raimundo, Juliana Macedo Rodrigues, Raisa Braul Peixoto, Maristela Peckle Santos, Huarrisson Azevedo André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] Baldani, Cristiane Divan |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro - UFRRJ Universidade Federal Fluminense (UFF) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guimarães, Andresa Raimundo, Juliana Macedo Rodrigues, Raisa Braul Peixoto, Maristela Peckle Santos, Huarrisson Azevedo André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] Baldani, Cristiane Divan |
dc.subject.por.fl_str_mv |
Ehrlichiosis Feline Hematology IFA Nested PCR |
topic |
Ehrlichiosis Feline Hematology IFA Nested PCR |
description |
Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:23:55Z 2019-10-06T16:23:55Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s1984-296120180088 Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019. 0103-846X http://hdl.handle.net/11449/188934 10.1590/s1984-296120180088 S1984-29612019000100180 2-s2.0-85063923138 S1984-29612019000100180.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1590/s1984-296120180088 http://hdl.handle.net/11449/188934 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 1, p. 180-185, 2019. 0103-846X 10.1590/s1984-296120180088 S1984-29612019000100180 2-s2.0-85063923138 S1984-29612019000100180.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
180-185 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129288801615872 |