Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros

Bibliographic Details
Main Author: Silva, Felipe Jorge da [UNESP]
Publication Date: 2014
Format: Doctoral thesis
Language: por
Source: Repositório Institucional da UNESP
Download full: http://hdl.handle.net/11449/121909
Summary: Family farming properties are important places to be considered units of study in the field of Preventive Veterinary Medicine because their environment and handling characteristics of animals typically employed promote the contact between the different species and thus there are greater chances of transmission of infectious agents with zoonotic potential. The objective of this study was to describe epidemiological aspects of the Leptospira spp. infection and of the occurrence of antibodies against Leptospira spp. in humans and animals populations in farms in the Brazilian biomes. Were harvested from free-living wild animals, domestic animals and humans, samples of blood and urine. For serological analysis, the microscopic agglutination test (MAT) was performed. Urine samples were seeded on media Ellighausen, McCullough, Johnson and Harris culture - DifcoR (EMJH) and Fletcher - DifcoR. Samples of which was isolated the bacteria were genotyped, using the technique Multiple Locus Variable number tandem repeat (MLVA), in Leptospirosis Laboratory of the Institute of Pathobiology, Center for Research in Veterinary and Agricultural Sciences, National Institute of Agricultural Technology (INTA), Buenos Aires, Argentina. To determine the risk factors of leptospirosis, a questionnaire was applied to the people of each property. Univariate analysis was performed using the Fisher exact test in order to verify the association of each of the possible risk factors with the dependent variable. Subsequently, was performed a multivariate analysis, which was built a model of logistic regression. In different species, serovars of Leptospira spp. against which antibody titers were found were Icterohaemorrhagiae, Copenhageni, Grippotyphosa, Pomona, Canicola, Wolffi, Hardjo, Panama, tarassovi, Hebdomadis, Shermani, Bratislava, Bataviae, Australis, Castellonis, Andamana, Autumnalis, Pyrogenes, Whiticombi, Javanica and Patoc. There was isolation of ...
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spelling Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileirosLeptospiraInfecçãoPropriedade ruralEpidemiologiaFatores de riscoFarm ownershipFamily farming properties are important places to be considered units of study in the field of Preventive Veterinary Medicine because their environment and handling characteristics of animals typically employed promote the contact between the different species and thus there are greater chances of transmission of infectious agents with zoonotic potential. The objective of this study was to describe epidemiological aspects of the Leptospira spp. infection and of the occurrence of antibodies against Leptospira spp. in humans and animals populations in farms in the Brazilian biomes. Were harvested from free-living wild animals, domestic animals and humans, samples of blood and urine. For serological analysis, the microscopic agglutination test (MAT) was performed. Urine samples were seeded on media Ellighausen, McCullough, Johnson and Harris culture - DifcoR (EMJH) and Fletcher - DifcoR. Samples of which was isolated the bacteria were genotyped, using the technique Multiple Locus Variable number tandem repeat (MLVA), in Leptospirosis Laboratory of the Institute of Pathobiology, Center for Research in Veterinary and Agricultural Sciences, National Institute of Agricultural Technology (INTA), Buenos Aires, Argentina. To determine the risk factors of leptospirosis, a questionnaire was applied to the people of each property. Univariate analysis was performed using the Fisher exact test in order to verify the association of each of the possible risk factors with the dependent variable. Subsequently, was performed a multivariate analysis, which was built a model of logistic regression. In different species, serovars of Leptospira spp. against which antibody titers were found were Icterohaemorrhagiae, Copenhageni, Grippotyphosa, Pomona, Canicola, Wolffi, Hardjo, Panama, tarassovi, Hebdomadis, Shermani, Bratislava, Bataviae, Australis, Castellonis, Andamana, Autumnalis, Pyrogenes, Whiticombi, Javanica and Patoc. There was isolation of ...Propriedades de agricultura familiar são importantes locais a serem considerados como unidades de estudos na área de medicina veterinária preventiva, pois seu ambiente e as características de manejo dos animais normalmente empregadas favorecem o contato entre as diferentes espécies e, dessa forma, há maiores chances de transmissão de agentes infecciosos com potencial zoonótico. O objetivo do trabalho foi descrever aspectos epidemiológicos da infecção por Leptospira spp. e da ocorrência de anticorpos contra Leptospira spp. em populações humanas e animais em propriedades rurais nos biomas brasileiros. Foram colhidas amostras de sangue e de urina de animais selvagens de vida livre, animais domésticos e de seres humanos. Para o diagnóstico sorológico de leptospirose, foi realizada a prova de soroaglutinação microscópica (SAM). As amostras de urina foram semeadas em meios de cultura Ellighausen, McCullough, Johnson e Harris - DifcoR (EMJH) e Fletcher - DifcoR. As amostras das quais houve isolamento das bactérias foram genotipadas, utilizando a técnica Multiple Locus Variable number tandem repeat (MLVA), no Laboratório de Leptospirose do Instituto de Patobiologia, Centro de Investigação em Ciências Veterinárias e Agronômicas, do Instituto Nacional de Tecnologia Agropecuária (INTA), Buenos Aires, Argentina. Para a determinação dos fatores de risco de leptospirose, foi aplicado um questionário aos indivíduos de cada propriedade. Realizou-se uma análise univariada, utilizando- se o teste exato de Fisher, a fim de verificar a associação de cada um dos possíveis fatores de risco com a variável dependente. Posteriormente foi realizada uma análise multivariada, na qual foi construído um modelo de regressão logística. Nas diferentes espécies, as sorovariedades de Leptospira spp. contra as quais foram encontrados títulos de anticorpos foram Icterohaemorrhagiae, Copenhageni, Grippotyphosa, Pomona, Canicola ...Universidade Estadual Paulista (Unesp)Mathias, Luis Antonio [UNESP]Universidade Estadual Paulista (Unesp)Silva, Felipe Jorge da [UNESP]2015-04-09T12:28:10Z2015-04-09T12:28:10Z2014-07-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisxv, 155 p. : il.application/pdfSILVA, Felipe Jorge da. Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros. 2014. xv, 155 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.http://hdl.handle.net/11449/121909000816477000816477.pdf33004102072P9Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-24T06:13:50Zoai:repositorio.unesp.br:11449/121909Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-24T06:13:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
title Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
spellingShingle Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
Silva, Felipe Jorge da [UNESP]
Leptospira
Infecção
Propriedade rural
Epidemiologia
Fatores de risco
Farm ownership
title_short Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
title_full Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
title_fullStr Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
title_full_unstemmed Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
title_sort Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros
author Silva, Felipe Jorge da [UNESP]
author_facet Silva, Felipe Jorge da [UNESP]
author_role author
dc.contributor.none.fl_str_mv Mathias, Luis Antonio [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Felipe Jorge da [UNESP]
dc.subject.por.fl_str_mv Leptospira
Infecção
Propriedade rural
Epidemiologia
Fatores de risco
Farm ownership
topic Leptospira
Infecção
Propriedade rural
Epidemiologia
Fatores de risco
Farm ownership
description Family farming properties are important places to be considered units of study in the field of Preventive Veterinary Medicine because their environment and handling characteristics of animals typically employed promote the contact between the different species and thus there are greater chances of transmission of infectious agents with zoonotic potential. The objective of this study was to describe epidemiological aspects of the Leptospira spp. infection and of the occurrence of antibodies against Leptospira spp. in humans and animals populations in farms in the Brazilian biomes. Were harvested from free-living wild animals, domestic animals and humans, samples of blood and urine. For serological analysis, the microscopic agglutination test (MAT) was performed. Urine samples were seeded on media Ellighausen, McCullough, Johnson and Harris culture - DifcoR (EMJH) and Fletcher - DifcoR. Samples of which was isolated the bacteria were genotyped, using the technique Multiple Locus Variable number tandem repeat (MLVA), in Leptospirosis Laboratory of the Institute of Pathobiology, Center for Research in Veterinary and Agricultural Sciences, National Institute of Agricultural Technology (INTA), Buenos Aires, Argentina. To determine the risk factors of leptospirosis, a questionnaire was applied to the people of each property. Univariate analysis was performed using the Fisher exact test in order to verify the association of each of the possible risk factors with the dependent variable. Subsequently, was performed a multivariate analysis, which was built a model of logistic regression. In different species, serovars of Leptospira spp. against which antibody titers were found were Icterohaemorrhagiae, Copenhageni, Grippotyphosa, Pomona, Canicola, Wolffi, Hardjo, Panama, tarassovi, Hebdomadis, Shermani, Bratislava, Bataviae, Australis, Castellonis, Andamana, Autumnalis, Pyrogenes, Whiticombi, Javanica and Patoc. There was isolation of ...
publishDate 2014
dc.date.none.fl_str_mv 2014-07-11
2015-04-09T12:28:10Z
2015-04-09T12:28:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Felipe Jorge da. Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros. 2014. xv, 155 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.
http://hdl.handle.net/11449/121909
000816477
000816477.pdf
33004102072P9
identifier_str_mv SILVA, Felipe Jorge da. Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros. 2014. xv, 155 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.
000816477
000816477.pdf
33004102072P9
url http://hdl.handle.net/11449/121909
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv xv, 155 p. : il.
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dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
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