Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros

• Main Author: Silva, Felipe Jorge da [UNESP]
• Publication Date: 2014
• Format: Doctoral thesis
• Language: por
• Source: Repositório Institucional da UNESP
• Download full: http://hdl.handle.net/11449/121909
• topic: Leptospira; Infecção; Propriedade rural; Epidemiologia; Fatores de risco; Farm ownership

Family farming properties are important places to be considered units of study in the field of Preventive Veterinary Medicine because their environment and handling characteristics of animals typically employed promote the contact between the different species and thus there are greater chances of transmission of infectious agents with zoonotic potential. The objective of this study was to describe epidemiological aspects of the Leptospira spp. infection and of the occurrence of antibodies against Leptospira spp. in humans and animals populations in farms in the Brazilian biomes. Were harvested from free-living wild animals, domestic animals and humans, samples of blood and urine. For serological analysis, the microscopic agglutination test (MAT) was performed. Urine samples were seeded on media Ellighausen, McCullough, Johnson and Harris culture - DifcoR (EMJH) and Fletcher - DifcoR. Samples of which was isolated the bacteria were genotyped, using the technique Multiple Locus Variable number tandem repeat (MLVA), in Leptospirosis Laboratory of the Institute of Pathobiology, Center for Research in Veterinary and Agricultural Sciences, National Institute of Agricultural Technology (INTA), Buenos Aires, Argentina. To determine the risk factors of leptospirosis, a questionnaire was applied to the people of each property. Univariate analysis was performed using the Fisher exact test in order to verify the association of each of the possible risk factors with the dependent variable. Subsequently, was performed a multivariate analysis, which was built a model of logistic regression. In different species, serovars of Leptospira spp. against which antibody titers were found were Icterohaemorrhagiae, Copenhageni, Grippotyphosa, Pomona, Canicola, Wolffi, Hardjo, Panama, tarassovi, Hebdomadis, Shermani, Bratislava, Bataviae, Australis, Castellonis, Andamana, Autumnalis, Pyrogenes, Whiticombi, Javanica and Patoc. There was isolation of ...