Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fvets.2020.577775 http://hdl.handle.net/11449/209776 |
Resumo: | In vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (P/ET), pregnancy loss (PL), and calving rates in buffalo recipients. The studied factors were season, recipient parity, the synchronization protocol, the CL diameter, asynchrony between the embryo and the recipient, the day of the recipient estrous cycle, the embryo (fresh vs. vitrified), the day of embryo development, and the embryo stage. These retrospective data, from a program of in vitro produced embryos, were analyzed by logistic regression, and the odds ratio was also estimated. Two factors were related to P/ET and the calving rate: (1) progesterone associated to estradiol plus eCG protocol for fixed time ET tended to affect positively P/ET on day 30 (41.9 vs. 36.1%, respectively; P = 0.07; AOR = 1.28) and P/ET on day 60 (37.8 vs. 36.1%, respectively; P = 0.09; AOR = 1.08) compared to the Ovsynch protocol; and (2) the CL diameter (>= 14.5 mm) at transfer increased P/ET on day 30 (47.4 vs. 32.5%; P < 0.01; AOR = 1.87) and on day 60 (45.3 vs. 27.7%; P < 0.01; AOR = 2.16), and also the calving rate (37.9 vs. 21.7%; P < 0.01; AOR = 2.20). PL was greater when ET was done in the nonbreeding season compared to the breeding season (PL 30-60: 12.8 vs. 0.0%, P = 0.01; AOR > 999.99; PL 60-calving: 26.8 vs. 3.6%, P = 0.03; AOR = 9.90; and PL 30-calving: 36.2 vs. 3.6%, P = 0.01; AOR = 15.30). In conclusion, the data of our study indicated that the synchronization protocol, the CL diameter, and ET during the breeding season impacted the reproductive efficiency of buffalo recipients. |
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Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?embryo transferOPUIVEPfresh embryosvitrificationembryofetal mortalityIn vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (P/ET), pregnancy loss (PL), and calving rates in buffalo recipients. The studied factors were season, recipient parity, the synchronization protocol, the CL diameter, asynchrony between the embryo and the recipient, the day of the recipient estrous cycle, the embryo (fresh vs. vitrified), the day of embryo development, and the embryo stage. These retrospective data, from a program of in vitro produced embryos, were analyzed by logistic regression, and the odds ratio was also estimated. Two factors were related to P/ET and the calving rate: (1) progesterone associated to estradiol plus eCG protocol for fixed time ET tended to affect positively P/ET on day 30 (41.9 vs. 36.1%, respectively; P = 0.07; AOR = 1.28) and P/ET on day 60 (37.8 vs. 36.1%, respectively; P = 0.09; AOR = 1.08) compared to the Ovsynch protocol; and (2) the CL diameter (>= 14.5 mm) at transfer increased P/ET on day 30 (47.4 vs. 32.5%; P < 0.01; AOR = 1.87) and on day 60 (45.3 vs. 27.7%; P < 0.01; AOR = 2.16), and also the calving rate (37.9 vs. 21.7%; P < 0.01; AOR = 2.20). PL was greater when ET was done in the nonbreeding season compared to the breeding season (PL 30-60: 12.8 vs. 0.0%, P = 0.01; AOR > 999.99; PL 60-calving: 26.8 vs. 3.6%, P = 0.03; AOR = 9.90; and PL 30-calving: 36.2 vs. 3.6%, P = 0.01; AOR = 15.30). In conclusion, the data of our study indicated that the synchronization protocol, the CL diameter, and ET during the breeding season impacted the reproductive efficiency of buffalo recipients.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Estadual Paulista, Dept Patol Reprod & Saude Unica, Jaboticabal, BrazilCenatte Embrioes Soc Ltd, Pedro Leopoldo, BrazilUniv Napoli Federico II, Dipartimento Med Vet & Prod Anim, Naples, ItalyUniv Illinois, Dept Anim Sci, 328 Mumford Hall, Urbana, IL 61801 USAUniv Sao Paulo, Dept Reprod Anim, Sao Paulo, BrazilUniv Fed Juiz de Fora, Dept Med Vet, Juiz De Fora, BrazilUniv Fed Minas Gerais, Dept Med Vet Prevent, Belo Horizonte, MG, BrazilUniv Estadual Paulista, Dept Patol Reprod & Saude Unica, Jaboticabal, BrazilFAPEMIG: APQ-0591-5.04/08Frontiers Media SaUniversidade Estadual Paulista (Unesp)Cenatte Embrioes Soc LtdUniv Napoli Federico IIUniv IllinoisUniversidade de São Paulo (USP)Univ Fed Juiz de ForaUniversidade Federal de Minas Gerais (UFMG)Saliba, Wilson PardiniGimenes, Lindsay Unno [UNESP]Drumond, Roberti MartinsBayao, Henrique Xavier SalgadoDi Palo, RossellaGasparrini, BiancaRubessa, MarcelloBaruselli, Pietro SampaioSales, Jose Nelio SouzaBastianetto, EduardoLeite, Romulo CerqueiraAlvim, Mucio Tulio Teixeira2021-06-25T12:28:56Z2021-06-25T12:28:56Z2020-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.3389/fvets.2020.577775Frontiers In Veterinary Science. Lausanne: Frontiers Media Sa, v. 7, 9 p., 2020.http://hdl.handle.net/11449/20977610.3389/fvets.2020.577775WOS:000598871000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209776Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
title |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
spellingShingle |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? Saliba, Wilson Pardini embryo transfer OPU IVEP fresh embryos vitrification embryo fetal mortality |
title_short |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
title_full |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
title_fullStr |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
title_full_unstemmed |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
title_sort |
Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos? |
author |
Saliba, Wilson Pardini |
author_facet |
Saliba, Wilson Pardini Gimenes, Lindsay Unno [UNESP] Drumond, Roberti Martins Bayao, Henrique Xavier Salgado Di Palo, Rossella Gasparrini, Bianca Rubessa, Marcello Baruselli, Pietro Sampaio Sales, Jose Nelio Souza Bastianetto, Eduardo Leite, Romulo Cerqueira Alvim, Mucio Tulio Teixeira |
author_role |
author |
author2 |
Gimenes, Lindsay Unno [UNESP] Drumond, Roberti Martins Bayao, Henrique Xavier Salgado Di Palo, Rossella Gasparrini, Bianca Rubessa, Marcello Baruselli, Pietro Sampaio Sales, Jose Nelio Souza Bastianetto, Eduardo Leite, Romulo Cerqueira Alvim, Mucio Tulio Teixeira |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Cenatte Embrioes Soc Ltd Univ Napoli Federico II Univ Illinois Universidade de São Paulo (USP) Univ Fed Juiz de Fora Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Saliba, Wilson Pardini Gimenes, Lindsay Unno [UNESP] Drumond, Roberti Martins Bayao, Henrique Xavier Salgado Di Palo, Rossella Gasparrini, Bianca Rubessa, Marcello Baruselli, Pietro Sampaio Sales, Jose Nelio Souza Bastianetto, Eduardo Leite, Romulo Cerqueira Alvim, Mucio Tulio Teixeira |
dc.subject.por.fl_str_mv |
embryo transfer OPU IVEP fresh embryos vitrification embryo fetal mortality |
topic |
embryo transfer OPU IVEP fresh embryos vitrification embryo fetal mortality |
description |
In vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (P/ET), pregnancy loss (PL), and calving rates in buffalo recipients. The studied factors were season, recipient parity, the synchronization protocol, the CL diameter, asynchrony between the embryo and the recipient, the day of the recipient estrous cycle, the embryo (fresh vs. vitrified), the day of embryo development, and the embryo stage. These retrospective data, from a program of in vitro produced embryos, were analyzed by logistic regression, and the odds ratio was also estimated. Two factors were related to P/ET and the calving rate: (1) progesterone associated to estradiol plus eCG protocol for fixed time ET tended to affect positively P/ET on day 30 (41.9 vs. 36.1%, respectively; P = 0.07; AOR = 1.28) and P/ET on day 60 (37.8 vs. 36.1%, respectively; P = 0.09; AOR = 1.08) compared to the Ovsynch protocol; and (2) the CL diameter (>= 14.5 mm) at transfer increased P/ET on day 30 (47.4 vs. 32.5%; P < 0.01; AOR = 1.87) and on day 60 (45.3 vs. 27.7%; P < 0.01; AOR = 2.16), and also the calving rate (37.9 vs. 21.7%; P < 0.01; AOR = 2.20). PL was greater when ET was done in the nonbreeding season compared to the breeding season (PL 30-60: 12.8 vs. 0.0%, P = 0.01; AOR > 999.99; PL 60-calving: 26.8 vs. 3.6%, P = 0.03; AOR = 9.90; and PL 30-calving: 36.2 vs. 3.6%, P = 0.01; AOR = 15.30). In conclusion, the data of our study indicated that the synchronization protocol, the CL diameter, and ET during the breeding season impacted the reproductive efficiency of buffalo recipients. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-02 2021-06-25T12:28:56Z 2021-06-25T12:28:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fvets.2020.577775 Frontiers In Veterinary Science. Lausanne: Frontiers Media Sa, v. 7, 9 p., 2020. http://hdl.handle.net/11449/209776 10.3389/fvets.2020.577775 WOS:000598871000001 |
url |
http://dx.doi.org/10.3389/fvets.2020.577775 http://hdl.handle.net/11449/209776 |
identifier_str_mv |
Frontiers In Veterinary Science. Lausanne: Frontiers Media Sa, v. 7, 9 p., 2020. 10.3389/fvets.2020.577775 WOS:000598871000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790294292299776 |