Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.parint.2019.01.009 http://hdl.handle.net/11449/187333 |
Resumo: | Henneguya guanduensis was originally described as a gill parasite of the Hoplosternum litoralle, a siluriform fish belonging to the Callichthyidae family. A study was undertaken with 58 specimens of H. litoralle taken from Batalha River in the state of São Paulo. The fish were collected and examined searching for lesions and/or myxosporean plasmodia. The prevalence of infection was 9.31% in the gills and 5.17% in the intestine. The mature spores had elongated bodies with polar capsules of inequal size and a caudal length greater than body length. Morphological characteristics identified the parasite as H. guanduensis. Molecular analysis of the SSU rDNA partials sequences resulted in a 1796 bp and 1712 bp for gills and intestine repectively, demonstrating significant genetic differences with previously described species of Henneguya and 99.7% similarity to each other when aligned. Phylogenetic analysis comparing the SSU rDNA sequence of H. guanduensis with closest species as indicated by BLASTn Max Score showed H. guanduensis as sister species of H. loretoensis in a subclade compose by species that parasites fishes from Amazon basin. This is the first report of the finding of Henneguya guanduensis spores in the intestine of Hoplosternum littorale. |
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Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular dataCallichthyidaeCatfishMyxobolidaePhylogenyUltrastructureHenneguya guanduensis was originally described as a gill parasite of the Hoplosternum litoralle, a siluriform fish belonging to the Callichthyidae family. A study was undertaken with 58 specimens of H. litoralle taken from Batalha River in the state of São Paulo. The fish were collected and examined searching for lesions and/or myxosporean plasmodia. The prevalence of infection was 9.31% in the gills and 5.17% in the intestine. The mature spores had elongated bodies with polar capsules of inequal size and a caudal length greater than body length. Morphological characteristics identified the parasite as H. guanduensis. Molecular analysis of the SSU rDNA partials sequences resulted in a 1796 bp and 1712 bp for gills and intestine repectively, demonstrating significant genetic differences with previously described species of Henneguya and 99.7% similarity to each other when aligned. Phylogenetic analysis comparing the SSU rDNA sequence of H. guanduensis with closest species as indicated by BLASTn Max Score showed H. guanduensis as sister species of H. loretoensis in a subclade compose by species that parasites fishes from Amazon basin. This is the first report of the finding of Henneguya guanduensis spores in the intestine of Hoplosternum littorale.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departament of Parasitology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESPCentro Universitário CESMACDepartament of Parasitology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESPFAPESP: 2014/12862-0FAPESP: 2015/24901-2Universidade Estadual Paulista (Unesp)Centro Universitário CESMACVieira, Diego Henrique Mirandola Dias [UNESP]Pelegrini, Larissa Sbeghen [UNESP]Abdallah, Vanessa Dorode Azevedo, Rodney Kozlowiski2019-10-06T15:32:52Z2019-10-06T15:32:52Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-32http://dx.doi.org/10.1016/j.parint.2019.01.009Parasitology International, v. 70, p. 27-32.1873-03291383-5769http://hdl.handle.net/11449/18733310.1016/j.parint.2019.01.0092-s2.0-85061294308Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Internationalinfo:eu-repo/semantics/openAccess2021-10-23T19:23:44Zoai:repositorio.unesp.br:11449/187333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
title |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
spellingShingle |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data Vieira, Diego Henrique Mirandola Dias [UNESP] Callichthyidae Catfish Myxobolidae Phylogeny Ultrastructure |
title_short |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
title_full |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
title_fullStr |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
title_full_unstemmed |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
title_sort |
Supplementary studies on Henneguya guanduensis (Cnidaria: Myxosporea) infecting gills and intestine of Hoplosternum littorale in Brazil: Ultrastructural and molecular data |
author |
Vieira, Diego Henrique Mirandola Dias [UNESP] |
author_facet |
Vieira, Diego Henrique Mirandola Dias [UNESP] Pelegrini, Larissa Sbeghen [UNESP] Abdallah, Vanessa Doro de Azevedo, Rodney Kozlowiski |
author_role |
author |
author2 |
Pelegrini, Larissa Sbeghen [UNESP] Abdallah, Vanessa Doro de Azevedo, Rodney Kozlowiski |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Centro Universitário CESMAC |
dc.contributor.author.fl_str_mv |
Vieira, Diego Henrique Mirandola Dias [UNESP] Pelegrini, Larissa Sbeghen [UNESP] Abdallah, Vanessa Doro de Azevedo, Rodney Kozlowiski |
dc.subject.por.fl_str_mv |
Callichthyidae Catfish Myxobolidae Phylogeny Ultrastructure |
topic |
Callichthyidae Catfish Myxobolidae Phylogeny Ultrastructure |
description |
Henneguya guanduensis was originally described as a gill parasite of the Hoplosternum litoralle, a siluriform fish belonging to the Callichthyidae family. A study was undertaken with 58 specimens of H. litoralle taken from Batalha River in the state of São Paulo. The fish were collected and examined searching for lesions and/or myxosporean plasmodia. The prevalence of infection was 9.31% in the gills and 5.17% in the intestine. The mature spores had elongated bodies with polar capsules of inequal size and a caudal length greater than body length. Morphological characteristics identified the parasite as H. guanduensis. Molecular analysis of the SSU rDNA partials sequences resulted in a 1796 bp and 1712 bp for gills and intestine repectively, demonstrating significant genetic differences with previously described species of Henneguya and 99.7% similarity to each other when aligned. Phylogenetic analysis comparing the SSU rDNA sequence of H. guanduensis with closest species as indicated by BLASTn Max Score showed H. guanduensis as sister species of H. loretoensis in a subclade compose by species that parasites fishes from Amazon basin. This is the first report of the finding of Henneguya guanduensis spores in the intestine of Hoplosternum littorale. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:32:52Z 2019-10-06T15:32:52Z 2019-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.parint.2019.01.009 Parasitology International, v. 70, p. 27-32. 1873-0329 1383-5769 http://hdl.handle.net/11449/187333 10.1016/j.parint.2019.01.009 2-s2.0-85061294308 |
url |
http://dx.doi.org/10.1016/j.parint.2019.01.009 http://hdl.handle.net/11449/187333 |
identifier_str_mv |
Parasitology International, v. 70, p. 27-32. 1873-0329 1383-5769 10.1016/j.parint.2019.01.009 2-s2.0-85061294308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-32 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962233137889280 |