Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11686-021-00454-9 http://hdl.handle.net/11449/229181 |
Resumo: | Purpose: We describe two new Henneguya spp. (Myxobolidae) found parasitizing Cyphocharax modestus from Pardo river, Paraná river basin, municipality of Botucatu, São Paulo State, Brazil: Henneguya fastigata n. sp. from gill lamellae and Henneguya pardensis n. sp. from gill arches. We based the descriptions on myxospore morphology, histology, and small subunit ribosomal DNA sequences. Methods: In this investigation, 50 specimens of Cyphocharax modestus were examined between July and December 2020 for myxozoan infections. Morphological characterization was based on the mature myxospores. The small subunit ribosomal DNA sequences were used for molecular and phylogenetic study. Results: Phylogenetic analysis provided the evolutionary position of these new species with other myxobolids species. Myxospores of the two species had similar morphology and morphometrics, but differed in spore body width and length, and ssrDNA sequences differed by 7.2%. These data supported the diagnosis of the parasites as distinct and novel species. The phylogenetic analysis showed a well-supported subclade formed by species that parasitize curimatid fishes, with Henneguya gilbert as a sister species of Henneguya fastigata n. sp., and Henneguya pardensis n. sp. as a sister species of both species. Conclusion: Our analysis was consistent with previous studies suggesting that orders and families of the hosts are strongly correlated with phylogenetic signals in the Myxobolidae. These are the first species of myxozoans described in the Pardo river. |
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Repositório Institucional da UNESP |
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Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular AnalysesCurimatidaeGillsMyxobolidaePardo riverPhylogenyPurpose: We describe two new Henneguya spp. (Myxobolidae) found parasitizing Cyphocharax modestus from Pardo river, Paraná river basin, municipality of Botucatu, São Paulo State, Brazil: Henneguya fastigata n. sp. from gill lamellae and Henneguya pardensis n. sp. from gill arches. We based the descriptions on myxospore morphology, histology, and small subunit ribosomal DNA sequences. Methods: In this investigation, 50 specimens of Cyphocharax modestus were examined between July and December 2020 for myxozoan infections. Morphological characterization was based on the mature myxospores. The small subunit ribosomal DNA sequences were used for molecular and phylogenetic study. Results: Phylogenetic analysis provided the evolutionary position of these new species with other myxobolids species. Myxospores of the two species had similar morphology and morphometrics, but differed in spore body width and length, and ssrDNA sequences differed by 7.2%. These data supported the diagnosis of the parasites as distinct and novel species. The phylogenetic analysis showed a well-supported subclade formed by species that parasitize curimatid fishes, with Henneguya gilbert as a sister species of Henneguya fastigata n. sp., and Henneguya pardensis n. sp. as a sister species of both species. Conclusion: Our analysis was consistent with previous studies suggesting that orders and families of the hosts are strongly correlated with phylogenetic signals in the Myxobolidae. These are the first species of myxozoans described in the Pardo river.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratory of Parasitology of Wild Animals Division of Parasitology Institute of Biosciences Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Rubião Junior, SPPrograma de Pós-Graduação Em Análise de Sistemas Ambientais Centro Universitário CESMAC, ALLaboratory of Parasitology of Wild Animals Division of Parasitology Institute of Biosciences Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Rubião Junior, SPFAPESP: 2019/19060-0FAPESP: 2019/26831-2FAPESP: 2020/05412-9CNPq: 309125/2017-0Universidade Estadual Paulista (UNESP)Centro Universitário CESMACVieira, Diego Henrique Mirandola Dias [UNESP]Narciso, Rodrigo Bravin [UNESP]de Azevedo, Rodney Kozlowiskida Silva, Reinaldo José [UNESP]2022-04-29T08:30:51Z2022-04-29T08:30:51Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article233-243http://dx.doi.org/10.1007/s11686-021-00454-9Acta Parasitologica, v. 67, n. 1, p. 233-243, 2022.1896-18511230-2821http://hdl.handle.net/11449/22918110.1007/s11686-021-00454-92-s2.0-85110713292Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Parasitologicainfo:eu-repo/semantics/openAccess2022-04-29T08:30:51Zoai:repositorio.unesp.br:11449/229181Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:30:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
title |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
spellingShingle |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses Vieira, Diego Henrique Mirandola Dias [UNESP] Curimatidae Gills Myxobolidae Pardo river Phylogeny |
title_short |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
title_full |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
title_fullStr |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
title_full_unstemmed |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
title_sort |
Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses |
author |
Vieira, Diego Henrique Mirandola Dias [UNESP] |
author_facet |
Vieira, Diego Henrique Mirandola Dias [UNESP] Narciso, Rodrigo Bravin [UNESP] de Azevedo, Rodney Kozlowiski da Silva, Reinaldo José [UNESP] |
author_role |
author |
author2 |
Narciso, Rodrigo Bravin [UNESP] de Azevedo, Rodney Kozlowiski da Silva, Reinaldo José [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Centro Universitário CESMAC |
dc.contributor.author.fl_str_mv |
Vieira, Diego Henrique Mirandola Dias [UNESP] Narciso, Rodrigo Bravin [UNESP] de Azevedo, Rodney Kozlowiski da Silva, Reinaldo José [UNESP] |
dc.subject.por.fl_str_mv |
Curimatidae Gills Myxobolidae Pardo river Phylogeny |
topic |
Curimatidae Gills Myxobolidae Pardo river Phylogeny |
description |
Purpose: We describe two new Henneguya spp. (Myxobolidae) found parasitizing Cyphocharax modestus from Pardo river, Paraná river basin, municipality of Botucatu, São Paulo State, Brazil: Henneguya fastigata n. sp. from gill lamellae and Henneguya pardensis n. sp. from gill arches. We based the descriptions on myxospore morphology, histology, and small subunit ribosomal DNA sequences. Methods: In this investigation, 50 specimens of Cyphocharax modestus were examined between July and December 2020 for myxozoan infections. Morphological characterization was based on the mature myxospores. The small subunit ribosomal DNA sequences were used for molecular and phylogenetic study. Results: Phylogenetic analysis provided the evolutionary position of these new species with other myxobolids species. Myxospores of the two species had similar morphology and morphometrics, but differed in spore body width and length, and ssrDNA sequences differed by 7.2%. These data supported the diagnosis of the parasites as distinct and novel species. The phylogenetic analysis showed a well-supported subclade formed by species that parasitize curimatid fishes, with Henneguya gilbert as a sister species of Henneguya fastigata n. sp., and Henneguya pardensis n. sp. as a sister species of both species. Conclusion: Our analysis was consistent with previous studies suggesting that orders and families of the hosts are strongly correlated with phylogenetic signals in the Myxobolidae. These are the first species of myxozoans described in the Pardo river. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:30:51Z 2022-04-29T08:30:51Z 2022-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11686-021-00454-9 Acta Parasitologica, v. 67, n. 1, p. 233-243, 2022. 1896-1851 1230-2821 http://hdl.handle.net/11449/229181 10.1007/s11686-021-00454-9 2-s2.0-85110713292 |
url |
http://dx.doi.org/10.1007/s11686-021-00454-9 http://hdl.handle.net/11449/229181 |
identifier_str_mv |
Acta Parasitologica, v. 67, n. 1, p. 233-243, 2022. 1896-1851 1230-2821 10.1007/s11686-021-00454-9 2-s2.0-85110713292 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Parasitologica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
233-243 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792961592925618176 |