Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232754 |
Resumo: | This study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours. The biofilms were exposed to the mouthwashes for 2 different lengths of time: T1, the time recommended by the manufacturer (30 or 60 seconds); and T2, double the recommended time (60 or 120 seconds). All antiseptic mouthwashes caused a significant reduction of biofilm (P < 0.05) as well as a significant reduction of viable gingival fibroblasts (P < 0.05) with both exposure times (T1 and T2). It can be concluded that the commercial mouthwashes demonstrated effective antibiofilm activity; they were more effective on bacteria than on C albicans. A significant cytotoxic effect on gingival fibroblasts was also observed. |
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Repositório Institucional da UNESP |
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Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblastsThis study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours. The biofilms were exposed to the mouthwashes for 2 different lengths of time: T1, the time recommended by the manufacturer (30 or 60 seconds); and T2, double the recommended time (60 or 120 seconds). All antiseptic mouthwashes caused a significant reduction of biofilm (P < 0.05) as well as a significant reduction of viable gingival fibroblasts (P < 0.05) with both exposure times (T1 and T2). It can be concluded that the commercial mouthwashes demonstrated effective antibiofilm activity; they were more effective on bacteria than on C albicans. A significant cytotoxic effect on gingival fibroblasts was also observed.Department of Biosciences and Oral Diagnosis São Paulo State University Institute of Science and TechnologyDepartment of Biosciences and Oral Diagnosis São Paulo State University Institute of Science and TechnologyUniversidade Estadual Paulista (UNESP)De Oliveira, Jonatas Rafael [UNESP]Belato, Kely Karina [UNESP]De Oliveira, Felipe Eduardo [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Camargo, Samira Esteves Afonso [UNESP]De Oliveira, Luciane Dias [UNESP]2022-04-30T09:53:09Z2022-04-30T09:53:09Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article28-34General Dentistry, v. 66, n. 2, p. 28-34, 2018.0363-6771http://hdl.handle.net/11449/2327542-s2.0-85048741527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneral Dentistryinfo:eu-repo/semantics/openAccess2022-04-30T09:53:09Zoai:repositorio.unesp.br:11449/232754Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-30T09:53:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
title |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
spellingShingle |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts De Oliveira, Jonatas Rafael [UNESP] |
title_short |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
title_full |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
title_fullStr |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
title_full_unstemmed |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
title_sort |
Mouthwashes: An in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts |
author |
De Oliveira, Jonatas Rafael [UNESP] |
author_facet |
De Oliveira, Jonatas Rafael [UNESP] Belato, Kely Karina [UNESP] De Oliveira, Felipe Eduardo [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Camargo, Samira Esteves Afonso [UNESP] De Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
Belato, Kely Karina [UNESP] De Oliveira, Felipe Eduardo [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Camargo, Samira Esteves Afonso [UNESP] De Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
De Oliveira, Jonatas Rafael [UNESP] Belato, Kely Karina [UNESP] De Oliveira, Felipe Eduardo [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Camargo, Samira Esteves Afonso [UNESP] De Oliveira, Luciane Dias [UNESP] |
description |
This study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours. The biofilms were exposed to the mouthwashes for 2 different lengths of time: T1, the time recommended by the manufacturer (30 or 60 seconds); and T2, double the recommended time (60 or 120 seconds). All antiseptic mouthwashes caused a significant reduction of biofilm (P < 0.05) as well as a significant reduction of viable gingival fibroblasts (P < 0.05) with both exposure times (T1 and T2). It can be concluded that the commercial mouthwashes demonstrated effective antibiofilm activity; they were more effective on bacteria than on C albicans. A significant cytotoxic effect on gingival fibroblasts was also observed. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 2022-04-30T09:53:09Z 2022-04-30T09:53:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
General Dentistry, v. 66, n. 2, p. 28-34, 2018. 0363-6771 http://hdl.handle.net/11449/232754 2-s2.0-85048741527 |
identifier_str_mv |
General Dentistry, v. 66, n. 2, p. 28-34, 2018. 0363-6771 2-s2.0-85048741527 |
url |
http://hdl.handle.net/11449/232754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
General Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
28-34 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789885759750144 |