Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0156947 http://hdl.handle.net/11449/173066 |
Resumo: | This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine� (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis. |
id |
UNSP_2ce8e2197ac64a3706458a58b48a9c7b |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/173066 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivoThis study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine� (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics Araraquara Dental School UNESP- Univ Estadual PaulistaDepartment of Physiology and Pathology Araraquara Dental School UNESP- Univ Estadual PaulistaPhysics Institute of S�o Carlos USP-University of S�o PauloDepartment of Dental Materials and Prosthodontics Araraquara Dental School UNESP- Univ Estadual PaulistaDepartment of Physiology and Pathology Araraquara Dental School UNESP- Univ Estadual PaulistaFAPESP: 2012/17468-2Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Carmello, Juliana Cabrini [UNESP]Alves, Fernanda [UNESP]Basso, Fernanda G. [UNESP]De Souza Costa, Carlos Alberto [UNESP]Bagnato, Vanderlei SalvadorMima, Ewerton Garcia De Oliveira [UNESP]Pavarina, Ana Cl�udia [UNESP]2018-12-11T17:03:21Z2018-12-11T17:03:21Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0156947PLoS ONE, v. 11, n. 6, 2016.1932-6203http://hdl.handle.net/11449/17306610.1371/journal.pone.01569472-s2.0-849736157462-s2.0-84973615746.pdf62599858990694980000-0002-9575-7625Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-01-07T06:31:21Zoai:repositorio.unesp.br:11449/173066Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-07T06:31:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
title |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
spellingShingle |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo Carmello, Juliana Cabrini [UNESP] |
title_short |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
title_full |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
title_fullStr |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
title_full_unstemmed |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
title_sort |
Treatment of oral candidiasis using Photodithazine�- mediated photodynamic therapy in vivo |
author |
Carmello, Juliana Cabrini [UNESP] |
author_facet |
Carmello, Juliana Cabrini [UNESP] Alves, Fernanda [UNESP] Basso, Fernanda G. [UNESP] De Souza Costa, Carlos Alberto [UNESP] Bagnato, Vanderlei Salvador Mima, Ewerton Garcia De Oliveira [UNESP] Pavarina, Ana Cl�udia [UNESP] |
author_role |
author |
author2 |
Alves, Fernanda [UNESP] Basso, Fernanda G. [UNESP] De Souza Costa, Carlos Alberto [UNESP] Bagnato, Vanderlei Salvador Mima, Ewerton Garcia De Oliveira [UNESP] Pavarina, Ana Cl�udia [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Carmello, Juliana Cabrini [UNESP] Alves, Fernanda [UNESP] Basso, Fernanda G. [UNESP] De Souza Costa, Carlos Alberto [UNESP] Bagnato, Vanderlei Salvador Mima, Ewerton Garcia De Oliveira [UNESP] Pavarina, Ana Cl�udia [UNESP] |
description |
This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine� (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:03:21Z 2018-12-11T17:03:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0156947 PLoS ONE, v. 11, n. 6, 2016. 1932-6203 http://hdl.handle.net/11449/173066 10.1371/journal.pone.0156947 2-s2.0-84973615746 2-s2.0-84973615746.pdf 6259985899069498 0000-0002-9575-7625 |
url |
http://dx.doi.org/10.1371/journal.pone.0156947 http://hdl.handle.net/11449/173066 |
identifier_str_mv |
PLoS ONE, v. 11, n. 6, 2016. 1932-6203 10.1371/journal.pone.0156947 2-s2.0-84973615746 2-s2.0-84973615746.pdf 6259985899069498 0000-0002-9575-7625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962282750214144 |