Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.bibliotecadigital.unicamp.br/document/?code=32595 http://hdl.handle.net/11449/70786 |
Resumo: | Aim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients. |
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Repositório Institucional da UNESP |
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Phenotypic characterization of Candida spp. isolates from chronic periodontitis patientsBiotypingCandida albicansMorphotypingPeriodontitisAim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients.Department of Oral Biosciences and Diagnosis Dental School of São José dos Campos São Paulo State University, Av. Eng. Francisco José Longo 777, 12245-000 - São José dos Campos, SPDepartment of Oral Biopathology Basic Institute of Biosciences University of Taubaté, TaubatéDepartment of Social Dentistry Dental School São Paulo State University, São PauloDepartment of Oral Biosciences and Diagnosis Dental School of São José dos Campos São Paulo State University, Av. Eng. Francisco José Longo 777, 12245-000 - São José dos Campos, SPDepartment of Social Dentistry Dental School São Paulo State University, São PauloUniversidade Estadual Paulista (Unesp)University of TaubatéKoga-Ito, Cristiane Yumi [UNESP]Komiyama, Edson Yukio [UNESP]Martins, Clélia Aparecida de Paiva [UNESP]dos Santos, Silvana Soléo FerreiraBalducci, Ivan [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]2014-05-27T11:23:47Z2014-05-27T11:23:47Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1531-1534application/pdfhttp://www.bibliotecadigital.unicamp.br/document/?code=32595Brazilian Journal of Oral Sciences, v. 7, n. 25, p. 1531-1534, 2008.1677-32171677-3225http://hdl.handle.net/11449/707862-s2.0-608490937662-s2.0-60849093766.pdf005356715362356965435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Oral Sciences0,131info:eu-repo/semantics/openAccess2023-10-22T06:08:50Zoai:repositorio.unesp.br:11449/70786Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-22T06:08:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
title |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
spellingShingle |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients Koga-Ito, Cristiane Yumi [UNESP] Biotyping Candida albicans Morphotyping Periodontitis |
title_short |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
title_full |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
title_fullStr |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
title_full_unstemmed |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
title_sort |
Phenotypic characterization of Candida spp. isolates from chronic periodontitis patients |
author |
Koga-Ito, Cristiane Yumi [UNESP] |
author_facet |
Koga-Ito, Cristiane Yumi [UNESP] Komiyama, Edson Yukio [UNESP] Martins, Clélia Aparecida de Paiva [UNESP] dos Santos, Silvana Soléo Ferreira Balducci, Ivan [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author_role |
author |
author2 |
Komiyama, Edson Yukio [UNESP] Martins, Clélia Aparecida de Paiva [UNESP] dos Santos, Silvana Soléo Ferreira Balducci, Ivan [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Taubaté |
dc.contributor.author.fl_str_mv |
Koga-Ito, Cristiane Yumi [UNESP] Komiyama, Edson Yukio [UNESP] Martins, Clélia Aparecida de Paiva [UNESP] dos Santos, Silvana Soléo Ferreira Balducci, Ivan [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
dc.subject.por.fl_str_mv |
Biotyping Candida albicans Morphotyping Periodontitis |
topic |
Biotyping Candida albicans Morphotyping Periodontitis |
description |
Aim: Several typing methods for Candida spp. have been suggested in the literature in order to distinguish isolates for studies about the virulence or infection routes of these microorganisms and, in particular, for epidemiological purposes. The aim of this study was to establish a comparison between the phenotypic profile of oral Candida isolates from periodontitis patients and control individuals. Methods: The morphotyping and biotyping of 35 C. albicans isolates obtained from chronic periodontitis patients and 48 isolates from control individuals were performed. For morphotyping, the isolates were plated on malt extract agar and incubated for 10 days. Sixteen different morphotypes were observed for C. albicans, the most frequently observed being 0000 and 0001. Results: Biotype 0000 (complete absence of fringe) was most prevalent among the isolates obtained from periodontitis patients compared to those from control individuals, with statistical significance. Biotyping revealed 5 different biotypes with higher prevalence of the biotype 357 among the isolates from control and periodontitis groups. Conclusions: The results obtained by biotyping of the isolates did not permit to differentiate a characteristic model related to periodontal disease, whilst the morphotype 0000 was most frequently isolated from periodontitis patients. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 2014-05-27T11:23:47Z 2014-05-27T11:23:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.bibliotecadigital.unicamp.br/document/?code=32595 Brazilian Journal of Oral Sciences, v. 7, n. 25, p. 1531-1534, 2008. 1677-3217 1677-3225 http://hdl.handle.net/11449/70786 2-s2.0-60849093766 2-s2.0-60849093766.pdf 0053567153623569 6543563161403421 0000-0002-2416-2173 |
url |
http://www.bibliotecadigital.unicamp.br/document/?code=32595 http://hdl.handle.net/11449/70786 |
identifier_str_mv |
Brazilian Journal of Oral Sciences, v. 7, n. 25, p. 1531-1534, 2008. 1677-3217 1677-3225 2-s2.0-60849093766 2-s2.0-60849093766.pdf 0053567153623569 6543563161403421 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Oral Sciences 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1531-1534 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964657119657984 |