A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Detalhes bibliográficos
Autor(a) principal: Santos, Adriano [UNESP]
Data de Publicação: 2018
Outros Autores: Bueno, Paulo R. [UNESP], Davis, Jason J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bios.2017.09.014
http://hdl.handle.net/11449/175275
Resumo: Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance).
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spelling A dual marker label free electrochemical assay for Flavivirus dengue diagnosisBiosensorDengueElectrochemical capacitance spectroscopyElectrochemical immittance analysisIgGMolecular diagnosticsNS1Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Royal Society of ChemistryPhysical Chemistry Department Institute of Chemistry São Paulo State University (Univ. Estadual Paulista UNESP) Nanobionics GroupDepartment of Chemistry University of Oxford, South Parks RoadPhysical Chemistry Department Institute of Chemistry São Paulo State University (Univ. Estadual Paulista UNESP) Nanobionics GroupFAPESP: 2012/22820-7Universidade Estadual Paulista (Unesp)University of OxfordSantos, Adriano [UNESP]Bueno, Paulo R. [UNESP]Davis, Jason J.2018-12-11T17:15:06Z2018-12-11T17:15:06Z2018-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article519-525application/pdfhttp://dx.doi.org/10.1016/j.bios.2017.09.014Biosensors and Bioelectronics, v. 100, p. 519-525.1873-42350956-5663http://hdl.handle.net/11449/17527510.1016/j.bios.2017.09.0142-s2.0-850302643552-s2.0-85030264355.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiosensors and Bioelectronics2,373info:eu-repo/semantics/openAccess2023-12-12T06:20:23Zoai:repositorio.unesp.br:11449/175275Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-12T06:20:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
title A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
spellingShingle A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
Santos, Adriano [UNESP]
Biosensor
Dengue
Electrochemical capacitance spectroscopy
Electrochemical immittance analysis
IgG
Molecular diagnostics
NS1
title_short A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
title_full A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
title_fullStr A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
title_full_unstemmed A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
title_sort A dual marker label free electrochemical assay for Flavivirus dengue diagnosis
author Santos, Adriano [UNESP]
author_facet Santos, Adriano [UNESP]
Bueno, Paulo R. [UNESP]
Davis, Jason J.
author_role author
author2 Bueno, Paulo R. [UNESP]
Davis, Jason J.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Oxford
dc.contributor.author.fl_str_mv Santos, Adriano [UNESP]
Bueno, Paulo R. [UNESP]
Davis, Jason J.
dc.subject.por.fl_str_mv Biosensor
Dengue
Electrochemical capacitance spectroscopy
Electrochemical immittance analysis
IgG
Molecular diagnostics
NS1
topic Biosensor
Dengue
Electrochemical capacitance spectroscopy
Electrochemical immittance analysis
IgG
Molecular diagnostics
NS1
description Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance).
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:15:06Z
2018-12-11T17:15:06Z
2018-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bios.2017.09.014
Biosensors and Bioelectronics, v. 100, p. 519-525.
1873-4235
0956-5663
http://hdl.handle.net/11449/175275
10.1016/j.bios.2017.09.014
2-s2.0-85030264355
2-s2.0-85030264355.pdf
url http://dx.doi.org/10.1016/j.bios.2017.09.014
http://hdl.handle.net/11449/175275
identifier_str_mv Biosensors and Bioelectronics, v. 100, p. 519-525.
1873-4235
0956-5663
10.1016/j.bios.2017.09.014
2-s2.0-85030264355
2-s2.0-85030264355.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biosensors and Bioelectronics
2,373
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 519-525
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797789979985838080

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