Optimized electrochemical biosensor for human prostatic acid phosphatase
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.snb.2017.06.035 http://hdl.handle.net/11449/165795 |
Resumo: | An innovative and sensitive label-free electrochemical biosensor platform for detecting human prostatic acid phosphatase (hPAP) is proposed herein. The methodology uses impedance-derived immittance functions (ImFs) for monitoring the interfacial changes in a hPAP bio-recognition layer constructed using a self-assembled monolayer of PEGlate thiol (low-fouling materials) and 11-ferrocenyl undecanethiol (redox probe confined). We evaluated the response toward the target primarily in phosphatase-buffered saline, at varying concentrations and across a wide range of frequencies. The optimum frequency for each ImF parameter was selected based on its sensitivity and linearity within a target concentration ranging from 20 pM to 5000 pM and a limit of detection (LoD) below the cut-off value for this cancer biomarker (10 pM). The best ImF selected (inverse of imaginary capacitance, 1/C?) was then successfully used to monitor hPAP in human blood serum and showed a linear range from 50 pM to 104 pM, with an LoD of 11.2 +/- 2.6 pM. Additionally, both the assay and analysis of the results can be performed in a few minutes without the need to add an interfering redox probe to the biological samples, thus providing a rapid, sensitive, and label-free test for prostate cancer. (C) 2017 Elsevier B.V. All rights reserved. |
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Repositório Institucional da UNESP |
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Optimized electrochemical biosensor for human prostatic acid phosphataseImmittance spectroscopyElectrochemical impedance spectroscopyProstate cancerHuman prostatic acid phosphataseLabel-free sensingElectrochemical biosensoraAn innovative and sensitive label-free electrochemical biosensor platform for detecting human prostatic acid phosphatase (hPAP) is proposed herein. The methodology uses impedance-derived immittance functions (ImFs) for monitoring the interfacial changes in a hPAP bio-recognition layer constructed using a self-assembled monolayer of PEGlate thiol (low-fouling materials) and 11-ferrocenyl undecanethiol (redox probe confined). We evaluated the response toward the target primarily in phosphatase-buffered saline, at varying concentrations and across a wide range of frequencies. The optimum frequency for each ImF parameter was selected based on its sensitivity and linearity within a target concentration ranging from 20 pM to 5000 pM and a limit of detection (LoD) below the cut-off value for this cancer biomarker (10 pM). The best ImF selected (inverse of imaginary capacitance, 1/C?) was then successfully used to monitor hPAP in human blood serum and showed a linear range from 50 pM to 104 pM, with an LoD of 11.2 +/- 2.6 pM. Additionally, both the assay and analysis of the results can be performed in a few minutes without the need to add an interfering redox probe to the biological samples, thus providing a rapid, sensitive, and label-free test for prostate cancer. (C) 2017 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Nanob Grp, Inst Chem, BR-14800900 Sao Paulo, BrazilSao Paulo State Univ, Nanob Grp, Inst Chem, BR-14800900 Sao Paulo, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Bedatty Fernandes, Flavio C. [UNESP]Bueno, Paulo R. [UNESP]2018-11-28T20:01:36Z2018-11-28T20:01:36Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1106-1112application/pdfhttp://dx.doi.org/10.1016/j.snb.2017.06.035Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 253, p. 1106-1112, 2017.0925-4005http://hdl.handle.net/11449/16579510.1016/j.snb.2017.06.035WOS:000411124800127WOS000411124800127.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors And Actuators B-chemicalinfo:eu-repo/semantics/openAccess2023-12-03T06:10:22Zoai:repositorio.unesp.br:11449/165795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-03T06:10:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
title |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
spellingShingle |
Optimized electrochemical biosensor for human prostatic acid phosphatase Bedatty Fernandes, Flavio C. [UNESP] Immittance spectroscopy Electrochemical impedance spectroscopy Prostate cancer Human prostatic acid phosphatase Label-free sensing Electrochemical biosensora |
title_short |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
title_full |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
title_fullStr |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
title_full_unstemmed |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
title_sort |
Optimized electrochemical biosensor for human prostatic acid phosphatase |
author |
Bedatty Fernandes, Flavio C. [UNESP] |
author_facet |
Bedatty Fernandes, Flavio C. [UNESP] Bueno, Paulo R. [UNESP] |
author_role |
author |
author2 |
Bueno, Paulo R. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bedatty Fernandes, Flavio C. [UNESP] Bueno, Paulo R. [UNESP] |
dc.subject.por.fl_str_mv |
Immittance spectroscopy Electrochemical impedance spectroscopy Prostate cancer Human prostatic acid phosphatase Label-free sensing Electrochemical biosensora |
topic |
Immittance spectroscopy Electrochemical impedance spectroscopy Prostate cancer Human prostatic acid phosphatase Label-free sensing Electrochemical biosensora |
description |
An innovative and sensitive label-free electrochemical biosensor platform for detecting human prostatic acid phosphatase (hPAP) is proposed herein. The methodology uses impedance-derived immittance functions (ImFs) for monitoring the interfacial changes in a hPAP bio-recognition layer constructed using a self-assembled monolayer of PEGlate thiol (low-fouling materials) and 11-ferrocenyl undecanethiol (redox probe confined). We evaluated the response toward the target primarily in phosphatase-buffered saline, at varying concentrations and across a wide range of frequencies. The optimum frequency for each ImF parameter was selected based on its sensitivity and linearity within a target concentration ranging from 20 pM to 5000 pM and a limit of detection (LoD) below the cut-off value for this cancer biomarker (10 pM). The best ImF selected (inverse of imaginary capacitance, 1/C?) was then successfully used to monitor hPAP in human blood serum and showed a linear range from 50 pM to 104 pM, with an LoD of 11.2 +/- 2.6 pM. Additionally, both the assay and analysis of the results can be performed in a few minutes without the need to add an interfering redox probe to the biological samples, thus providing a rapid, sensitive, and label-free test for prostate cancer. (C) 2017 Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-11-28T20:01:36Z 2018-11-28T20:01:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.snb.2017.06.035 Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 253, p. 1106-1112, 2017. 0925-4005 http://hdl.handle.net/11449/165795 10.1016/j.snb.2017.06.035 WOS:000411124800127 WOS000411124800127.pdf |
url |
http://dx.doi.org/10.1016/j.snb.2017.06.035 http://hdl.handle.net/11449/165795 |
identifier_str_mv |
Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 253, p. 1106-1112, 2017. 0925-4005 10.1016/j.snb.2017.06.035 WOS:000411124800127 WOS000411124800127.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sensors And Actuators B-chemical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1106-1112 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789887152259072 |