Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-04042-z http://hdl.handle.net/11449/221944 |
Resumo: | Objectives: This study investigated the influence of calcium hydroxide intracanal medications on the levels of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in apical periodontitis (AP). Materials and methods: Twenty primarily infected root canals with AP were randomly divided into two groups: Ca(OH)2 + sterile saline solution (SSL) group and Ca(OH)2 + 2% chlorhexidine gel (CHX gel) group. We collected samples from the periradicular tissue fluid (PTF) before (s1) and after 14 days of intracanal medication (s2). MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were measured by ELISA assay. Results: MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were detected in all PTF samples at s1 and s2 (20/20). At s1, MMP-2 and MMP-9 were detected at higher levels than MMP-1 (p <.05). Higher levels of TIMP-1 than TIMP-2 were found in AP (p <.05). Additionally, we detected higher MMP-1, MMP-2, and MMP-9 over TIMP-1 and TIMP-2 levels in AP (p <.05). At s2, Ca(OH)2 + SSL was as effective as Ca(OH)2 + 2% CHX gel in lowering the levels of MMP-1, MMP-2, and MMP-9 after 14 days of intracanal medication, with no significant difference between them (p >.05). Both Ca(OH) 2 intracanal medications had no significant impact on the levels of TIMP-1 and TIMP-2 (both p >.05). At s2, TIMP-1 levels were higher than TIMP-2 (p <.05). Moreover, there were positive correlations between the levels of MMP-1 and TIMP-1 and MMP-1 and TIMP-2 (p <.05). Conclusions: Calcium hydroxide medications effectively lowered the levels of MMP-1, MMP-2, and MMP-9 in periapical tissues after 14 days of treatment, with no difference between them. Moreover, the calcium hydroxide intracanal medications tested here had no impact in TIMP-1 and TIMP-2 in periapical tissues. Clinical relevance: MMPs and TIMPs play an essential role in the degradation of the extracellular matrix. The imbalance MMPs and TIMPs can cause periapical tissue destruction. Therefore, the reestablishment of the balance between activated MMPs and TIMPs with root canal therapy is essential to restore tissue homeostasis. |
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Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitisCalcium hydroxideIntracanal medicationMatrix metalloproteinaseRoot canalObjectives: This study investigated the influence of calcium hydroxide intracanal medications on the levels of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in apical periodontitis (AP). Materials and methods: Twenty primarily infected root canals with AP were randomly divided into two groups: Ca(OH)2 + sterile saline solution (SSL) group and Ca(OH)2 + 2% chlorhexidine gel (CHX gel) group. We collected samples from the periradicular tissue fluid (PTF) before (s1) and after 14 days of intracanal medication (s2). MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were measured by ELISA assay. Results: MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were detected in all PTF samples at s1 and s2 (20/20). At s1, MMP-2 and MMP-9 were detected at higher levels than MMP-1 (p <.05). Higher levels of TIMP-1 than TIMP-2 were found in AP (p <.05). Additionally, we detected higher MMP-1, MMP-2, and MMP-9 over TIMP-1 and TIMP-2 levels in AP (p <.05). At s2, Ca(OH)2 + SSL was as effective as Ca(OH)2 + 2% CHX gel in lowering the levels of MMP-1, MMP-2, and MMP-9 after 14 days of intracanal medication, with no significant difference between them (p >.05). Both Ca(OH) 2 intracanal medications had no significant impact on the levels of TIMP-1 and TIMP-2 (both p >.05). At s2, TIMP-1 levels were higher than TIMP-2 (p <.05). Moreover, there were positive correlations between the levels of MMP-1 and TIMP-1 and MMP-1 and TIMP-2 (p <.05). Conclusions: Calcium hydroxide medications effectively lowered the levels of MMP-1, MMP-2, and MMP-9 in periapical tissues after 14 days of treatment, with no difference between them. Moreover, the calcium hydroxide intracanal medications tested here had no impact in TIMP-1 and TIMP-2 in periapical tissues. Clinical relevance: MMPs and TIMPs play an essential role in the degradation of the extracellular matrix. The imbalance MMPs and TIMPs can cause periapical tissue destruction. Therefore, the reestablishment of the balance between activated MMPs and TIMPs with root canal therapy is essential to restore tissue homeostasis.Department of Restorative Dentistry Endodontic Division São Paulo State University (Unesp) Institute of Science and TechnologyDivision of Endodontics Department of Advanced Oral Sciences and Therapeutics University of Maryland School of Dentistry, 650 West Baltimore StreetDepartment of Restorative Dentistry Endodontic Division São Paulo State University (Unesp) Institute of Science and TechnologyUniversidade Estadual Paulista (UNESP)University of Maryland School of DentistryTeixeira, Flavia F. C. [UNESP]Cardoso, Flavia G. R. [UNESP]Ferreira, Nadia S. [UNESP]Gomes, Ana P. M. [UNESP]Corazza, Bruna J. M. [UNESP]Valera, Marcia C. [UNESP]Martinho, Frederico C.2022-04-28T19:41:30Z2022-04-28T19:41:30Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article643-650http://dx.doi.org/10.1007/s00784-021-04042-zClinical Oral Investigations, v. 26, n. 1, p. 643-650, 2022.1436-37711432-6981http://hdl.handle.net/11449/22194410.1007/s00784-021-04042-z2-s2.0-85109997925Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2022-04-28T19:41:30Zoai:repositorio.unesp.br:11449/221944Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:41:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
title |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
spellingShingle |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis Teixeira, Flavia F. C. [UNESP] Calcium hydroxide Intracanal medication Matrix metalloproteinase Root canal |
title_short |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
title_full |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
title_fullStr |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
title_full_unstemmed |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
title_sort |
Clinical influence of calcium hydroxide intracanal medications on matrix metalloproteinases and tissue inhibitors of metalloproteinases in apical periodontitis |
author |
Teixeira, Flavia F. C. [UNESP] |
author_facet |
Teixeira, Flavia F. C. [UNESP] Cardoso, Flavia G. R. [UNESP] Ferreira, Nadia S. [UNESP] Gomes, Ana P. M. [UNESP] Corazza, Bruna J. M. [UNESP] Valera, Marcia C. [UNESP] Martinho, Frederico C. |
author_role |
author |
author2 |
Cardoso, Flavia G. R. [UNESP] Ferreira, Nadia S. [UNESP] Gomes, Ana P. M. [UNESP] Corazza, Bruna J. M. [UNESP] Valera, Marcia C. [UNESP] Martinho, Frederico C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Maryland School of Dentistry |
dc.contributor.author.fl_str_mv |
Teixeira, Flavia F. C. [UNESP] Cardoso, Flavia G. R. [UNESP] Ferreira, Nadia S. [UNESP] Gomes, Ana P. M. [UNESP] Corazza, Bruna J. M. [UNESP] Valera, Marcia C. [UNESP] Martinho, Frederico C. |
dc.subject.por.fl_str_mv |
Calcium hydroxide Intracanal medication Matrix metalloproteinase Root canal |
topic |
Calcium hydroxide Intracanal medication Matrix metalloproteinase Root canal |
description |
Objectives: This study investigated the influence of calcium hydroxide intracanal medications on the levels of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in apical periodontitis (AP). Materials and methods: Twenty primarily infected root canals with AP were randomly divided into two groups: Ca(OH)2 + sterile saline solution (SSL) group and Ca(OH)2 + 2% chlorhexidine gel (CHX gel) group. We collected samples from the periradicular tissue fluid (PTF) before (s1) and after 14 days of intracanal medication (s2). MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were measured by ELISA assay. Results: MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-2 were detected in all PTF samples at s1 and s2 (20/20). At s1, MMP-2 and MMP-9 were detected at higher levels than MMP-1 (p <.05). Higher levels of TIMP-1 than TIMP-2 were found in AP (p <.05). Additionally, we detected higher MMP-1, MMP-2, and MMP-9 over TIMP-1 and TIMP-2 levels in AP (p <.05). At s2, Ca(OH)2 + SSL was as effective as Ca(OH)2 + 2% CHX gel in lowering the levels of MMP-1, MMP-2, and MMP-9 after 14 days of intracanal medication, with no significant difference between them (p >.05). Both Ca(OH) 2 intracanal medications had no significant impact on the levels of TIMP-1 and TIMP-2 (both p >.05). At s2, TIMP-1 levels were higher than TIMP-2 (p <.05). Moreover, there were positive correlations between the levels of MMP-1 and TIMP-1 and MMP-1 and TIMP-2 (p <.05). Conclusions: Calcium hydroxide medications effectively lowered the levels of MMP-1, MMP-2, and MMP-9 in periapical tissues after 14 days of treatment, with no difference between them. Moreover, the calcium hydroxide intracanal medications tested here had no impact in TIMP-1 and TIMP-2 in periapical tissues. Clinical relevance: MMPs and TIMPs play an essential role in the degradation of the extracellular matrix. The imbalance MMPs and TIMPs can cause periapical tissue destruction. Therefore, the reestablishment of the balance between activated MMPs and TIMPs with root canal therapy is essential to restore tissue homeostasis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:41:30Z 2022-04-28T19:41:30Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-04042-z Clinical Oral Investigations, v. 26, n. 1, p. 643-650, 2022. 1436-3771 1432-6981 http://hdl.handle.net/11449/221944 10.1007/s00784-021-04042-z 2-s2.0-85109997925 |
url |
http://dx.doi.org/10.1007/s00784-021-04042-z http://hdl.handle.net/11449/221944 |
identifier_str_mv |
Clinical Oral Investigations, v. 26, n. 1, p. 643-650, 2022. 1436-3771 1432-6981 10.1007/s00784-021-04042-z 2-s2.0-85109997925 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
643-650 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790147553525760 |