Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13403 http://hdl.handle.net/11449/205252 |
Resumo: | Aim: To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues. Methodology: Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH)2] + saline solution (SSL) [Ca(OH)2 + SSL group] (n = 12), Ca(OH)2 + 2% chlorhexidine gel [Ca(OH)2 + 2% CHX gel group] (n = 12) and NAC [NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal–Wallis test, followed by Dunn’s post hoc test; all statistical tests were performed at a significance level of 5%. Results: RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)2 medication did not increase the levels of RvE1 or RvD2 (both P > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05). Conclusions: RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment. |
id |
UNSP_857188c827826b86c2670b80b41e4932 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/205252 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitiscalcium hydroxideintracanal medicationresolvinsroot canalAim: To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues. Methodology: Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH)2] + saline solution (SSL) [Ca(OH)2 + SSL group] (n = 12), Ca(OH)2 + 2% chlorhexidine gel [Ca(OH)2 + 2% CHX gel group] (n = 12) and NAC [NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal–Wallis test, followed by Dunn’s post hoc test; all statistical tests were performed at a significance level of 5%. Results: RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)2 medication did not increase the levels of RvE1 or RvD2 (both P > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05). Conclusions: RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State University – UNESPDepartment of Advanced Oral Sciences and Therapeutics School of Dentistry University of MarylandDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State University – UNESPFAPESP: 2016/26012-3FAPESP: 2018/01703-9Universidade Estadual Paulista (Unesp)University of MarylandCorazza, B. J.M. [UNESP]Martinho, F. C.Khoury, R. D. [UNESP]Toia, C. C. [UNESP]Orozco, E. I.F. [UNESP]Prado, R. F. [UNESP]Machado, F. P. [UNESP]Valera, M. C. [UNESP]2021-06-25T10:12:18Z2021-06-25T10:12:18Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article61-73http://dx.doi.org/10.1111/iej.13403International Endodontic Journal, v. 54, n. 1, p. 61-73, 2021.1365-25910143-2885http://hdl.handle.net/11449/20525210.1111/iej.134032-s2.0-85092018945Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2021-10-23T12:23:59Zoai:repositorio.unesp.br:11449/205252Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:23:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
title |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
spellingShingle |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis Corazza, B. J.M. [UNESP] calcium hydroxide intracanal medication resolvins root canal |
title_short |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
title_full |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
title_fullStr |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
title_full_unstemmed |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
title_sort |
Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis |
author |
Corazza, B. J.M. [UNESP] |
author_facet |
Corazza, B. J.M. [UNESP] Martinho, F. C. Khoury, R. D. [UNESP] Toia, C. C. [UNESP] Orozco, E. I.F. [UNESP] Prado, R. F. [UNESP] Machado, F. P. [UNESP] Valera, M. C. [UNESP] |
author_role |
author |
author2 |
Martinho, F. C. Khoury, R. D. [UNESP] Toia, C. C. [UNESP] Orozco, E. I.F. [UNESP] Prado, R. F. [UNESP] Machado, F. P. [UNESP] Valera, M. C. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Maryland |
dc.contributor.author.fl_str_mv |
Corazza, B. J.M. [UNESP] Martinho, F. C. Khoury, R. D. [UNESP] Toia, C. C. [UNESP] Orozco, E. I.F. [UNESP] Prado, R. F. [UNESP] Machado, F. P. [UNESP] Valera, M. C. [UNESP] |
dc.subject.por.fl_str_mv |
calcium hydroxide intracanal medication resolvins root canal |
topic |
calcium hydroxide intracanal medication resolvins root canal |
description |
Aim: To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues. Methodology: Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH)2] + saline solution (SSL) [Ca(OH)2 + SSL group] (n = 12), Ca(OH)2 + 2% chlorhexidine gel [Ca(OH)2 + 2% CHX gel group] (n = 12) and NAC [NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal–Wallis test, followed by Dunn’s post hoc test; all statistical tests were performed at a significance level of 5%. Results: RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)2 medication did not increase the levels of RvE1 or RvD2 (both P > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05). Conclusions: RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:12:18Z 2021-06-25T10:12:18Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13403 International Endodontic Journal, v. 54, n. 1, p. 61-73, 2021. 1365-2591 0143-2885 http://hdl.handle.net/11449/205252 10.1111/iej.13403 2-s2.0-85092018945 |
url |
http://dx.doi.org/10.1111/iej.13403 http://hdl.handle.net/11449/205252 |
identifier_str_mv |
International Endodontic Journal, v. 54, n. 1, p. 61-73, 2021. 1365-2591 0143-2885 10.1111/iej.13403 2-s2.0-85092018945 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
61-73 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964896504315904 |