Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Detalhes bibliográficos
Autor(a) principal: Tirapelli, D. P.C.
Data de Publicação: 2020
Outros Autores: Cirino, M. L.A., da Silva, J. P., Gula, I. S., Tazima, M. F.G.S., Fazan, V. P.S., de Carvalho, C. A.M., Novais, P. C., Lizarte Neto, F. S., Durand, M. T., Schimming, B. C. [UNESP], Carlotti Junior, C. G., Colli, B. O., Tirapelli, L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/GMR18624
http://hdl.handle.net/11449/221713
Resumo: Cerebral ischemia is one of the main causes of morbidity and mortality as Abusive use of alcohol also causes health problems, along with considerable social and economic repercussions. We examined how these two conditions affect histopathological signs, morphometrics, expression of apoptosis-related proteins CASPASE 3 and BCL2, and serum gene expression of miRNA-21, in cerebral ischemia associated with a chronic alcoholism model in brain tissue (striatum, lateral and dorsolteral cortex). Fifty adult Wistar rats were divided into five groups: control (C), sham (S), ischemic (I), alcoholic (A) and ischemic/alcoholic groups (I+A). Brain samples were processed for histopathological, morphological, and immunohistochemical analyses for the expression of CASPASE 3 and BCL2, and blood was collected for analysis of miRNA-21 gene expression by real-time PCR. Histopathological changes such as neurons with pyknotic nuclei and diffuse neurons with loss of the contour of their cellular membranes and cytoplasmic edema, were observed in groups I and I+A in all three areas. Protein expression of CASPASE 3 was significantly higher than that of BCL2, with higher expression in groups I and I+A for these two proteins. Serum expression of miRNA-21 was low in all groups, and was slightly higher in groups I and I+A, with no significant differences. The histopathological and morphometric alterations, which were observed mainly in the ischemic animals, correlated with the expression of CASPASE 3. The expression of BCL2 was greater where histopathological changes and expression of CASPASE 3 were less evident. The I and I+A groups presented more histopathological changes, with greater neuronal loss and cerebral edema, and greater expression of CASPASE 3, suggesting that ischemia and alcoholism, when associated, can cause considerable injury and damage to brain tissue.
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spelling Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism modelAlcoholismApoptosisCerebral ischemiaMiRNA-21MorphologyCerebral ischemia is one of the main causes of morbidity and mortality as Abusive use of alcohol also causes health problems, along with considerable social and economic repercussions. We examined how these two conditions affect histopathological signs, morphometrics, expression of apoptosis-related proteins CASPASE 3 and BCL2, and serum gene expression of miRNA-21, in cerebral ischemia associated with a chronic alcoholism model in brain tissue (striatum, lateral and dorsolteral cortex). Fifty adult Wistar rats were divided into five groups: control (C), sham (S), ischemic (I), alcoholic (A) and ischemic/alcoholic groups (I+A). Brain samples were processed for histopathological, morphological, and immunohistochemical analyses for the expression of CASPASE 3 and BCL2, and blood was collected for analysis of miRNA-21 gene expression by real-time PCR. Histopathological changes such as neurons with pyknotic nuclei and diffuse neurons with loss of the contour of their cellular membranes and cytoplasmic edema, were observed in groups I and I+A in all three areas. Protein expression of CASPASE 3 was significantly higher than that of BCL2, with higher expression in groups I and I+A for these two proteins. Serum expression of miRNA-21 was low in all groups, and was slightly higher in groups I and I+A, with no significant differences. The histopathological and morphometric alterations, which were observed mainly in the ischemic animals, correlated with the expression of CASPASE 3. The expression of BCL2 was greater where histopathological changes and expression of CASPASE 3 were less evident. The I and I+A groups presented more histopathological changes, with greater neuronal loss and cerebral edema, and greater expression of CASPASE 3, suggesting that ischemia and alcoholism, when associated, can cause considerable injury and damage to brain tissue.Departamento de Cirurgia e Anatomia Universidade de São PauloFaculdade de TaquaritingaFaculdade de Medicina Universidade de Ribeirão Preto MedicinaDepartamento de Medicina Centro Universitário Barão de MauáUniversidade de MaríliaDepartamento de Anatomia Universidade Estadual de São PauloDepartamento de Anatomia Universidade Estadual de São PauloUniversidade de São Paulo (USP)Faculdade de TaquaritingaMedicinaCentro Universitário Barão de MauáUniversidade de MaríliaUniversidade Estadual Paulista (UNESP)Tirapelli, D. P.C.Cirino, M. L.A.da Silva, J. P.Gula, I. S.Tazima, M. F.G.S.Fazan, V. P.S.de Carvalho, C. A.M.Novais, P. C.Lizarte Neto, F. S.Durand, M. T.Schimming, B. C. [UNESP]Carlotti Junior, C. G.Colli, B. O.Tirapelli, L. F.2022-04-28T19:30:20Z2022-04-28T19:30:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4238/GMR18624Genetics and Molecular Research, v. 19, n. 4, 2020.1676-5680http://hdl.handle.net/11449/22171310.4238/GMR186242-s2.0-85103560248Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:30:20Zoai:repositorio.unesp.br:11449/221713Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:17:05.858579Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
title Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
spellingShingle Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
Tirapelli, D. P.C.
Alcoholism
Apoptosis
Cerebral ischemia
MiRNA-21
Morphology
title_short Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
title_full Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
title_fullStr Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
title_full_unstemmed Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
title_sort Morphological, immunohistochemical and mirna21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model
author Tirapelli, D. P.C.
author_facet Tirapelli, D. P.C.
Cirino, M. L.A.
da Silva, J. P.
Gula, I. S.
Tazima, M. F.G.S.
Fazan, V. P.S.
de Carvalho, C. A.M.
Novais, P. C.
Lizarte Neto, F. S.
Durand, M. T.
Schimming, B. C. [UNESP]
Carlotti Junior, C. G.
Colli, B. O.
Tirapelli, L. F.
author_role author
author2 Cirino, M. L.A.
da Silva, J. P.
Gula, I. S.
Tazima, M. F.G.S.
Fazan, V. P.S.
de Carvalho, C. A.M.
Novais, P. C.
Lizarte Neto, F. S.
Durand, M. T.
Schimming, B. C. [UNESP]
Carlotti Junior, C. G.
Colli, B. O.
Tirapelli, L. F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Faculdade de Taquaritinga
Medicina
Centro Universitário Barão de Mauá
Universidade de Marília
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Tirapelli, D. P.C.
Cirino, M. L.A.
da Silva, J. P.
Gula, I. S.
Tazima, M. F.G.S.
Fazan, V. P.S.
de Carvalho, C. A.M.
Novais, P. C.
Lizarte Neto, F. S.
Durand, M. T.
Schimming, B. C. [UNESP]
Carlotti Junior, C. G.
Colli, B. O.
Tirapelli, L. F.
dc.subject.por.fl_str_mv Alcoholism
Apoptosis
Cerebral ischemia
MiRNA-21
Morphology
topic Alcoholism
Apoptosis
Cerebral ischemia
MiRNA-21
Morphology
description Cerebral ischemia is one of the main causes of morbidity and mortality as Abusive use of alcohol also causes health problems, along with considerable social and economic repercussions. We examined how these two conditions affect histopathological signs, morphometrics, expression of apoptosis-related proteins CASPASE 3 and BCL2, and serum gene expression of miRNA-21, in cerebral ischemia associated with a chronic alcoholism model in brain tissue (striatum, lateral and dorsolteral cortex). Fifty adult Wistar rats were divided into five groups: control (C), sham (S), ischemic (I), alcoholic (A) and ischemic/alcoholic groups (I+A). Brain samples were processed for histopathological, morphological, and immunohistochemical analyses for the expression of CASPASE 3 and BCL2, and blood was collected for analysis of miRNA-21 gene expression by real-time PCR. Histopathological changes such as neurons with pyknotic nuclei and diffuse neurons with loss of the contour of their cellular membranes and cytoplasmic edema, were observed in groups I and I+A in all three areas. Protein expression of CASPASE 3 was significantly higher than that of BCL2, with higher expression in groups I and I+A for these two proteins. Serum expression of miRNA-21 was low in all groups, and was slightly higher in groups I and I+A, with no significant differences. The histopathological and morphometric alterations, which were observed mainly in the ischemic animals, correlated with the expression of CASPASE 3. The expression of BCL2 was greater where histopathological changes and expression of CASPASE 3 were less evident. The I and I+A groups presented more histopathological changes, with greater neuronal loss and cerebral edema, and greater expression of CASPASE 3, suggesting that ischemia and alcoholism, when associated, can cause considerable injury and damage to brain tissue.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
2022-04-28T19:30:20Z
2022-04-28T19:30:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/GMR18624
Genetics and Molecular Research, v. 19, n. 4, 2020.
1676-5680
http://hdl.handle.net/11449/221713
10.4238/GMR18624
2-s2.0-85103560248
url http://dx.doi.org/10.4238/GMR18624
http://hdl.handle.net/11449/221713
identifier_str_mv Genetics and Molecular Research, v. 19, n. 4, 2020.
1676-5680
10.4238/GMR18624
2-s2.0-85103560248
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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