Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/gmr18637 http://hdl.handle.net/11449/209217 |
Resumo: | One of the main global causes of mortality is cerebral ischemia. Studies report that alcohol abuse causes health problems, aggravating the conditions of neurological diseases. The role of microRNAs has been highlighted in the literature as biomarkers in various types of diseases, including neurological disorders. Evidence of dysregulation of serum miRNAs related to apoptosis in experimental brain ischemia associated with alcoholism is scarce. We evaluated the gene expression of miRNA-15b and 16 (apoptotic) and miRNA-21, -221 and -222 (anti-apoptotic) in the blood after experimental induction of temporary focal ischemia (90 minutes) by occlusion of the middle cerebral artery in rats, followed by reperfusion for 48 hours, associated with a chronic alcoholism model. Fifty-six rats were randomly divided into seven experimental groups: Control (C); Sham (S); Ischemic (I); Ischemia-reperfusion (IR), Alcoholic (A); Alcoholic and ischemic (I + A); Ischemiareperfusion and Alcoholic (IR+A). The blood samples were collected for gene expression analysis of some serum miRNAs by PCR in real time. The serum expression of miRNA-16 was higher in the IR group compared to C and S groups (P < 0.05) but no association with chronic alcoholism was found. The serum expressions of miRNA-21, -221 and -222 were low in all groups and were not correlated with ischemic injury and/or chronic alcoholism. The serum expressions of miRNA-15b, -21, -221 and -222 were similar among the experimental groups, with no correlation with ischemia, with or without reperfusion, and/or alcoholism. The overexpression of miRNA-16 in the blood of I and IR groups demonstrated a correlation with the ischemic process, mainly after reperfusion for 48 hours, associated or not with alcoholism. |
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Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholismCerebral IschemiaApoptosisAlcoholismMorphologymiRNABloodOne of the main global causes of mortality is cerebral ischemia. Studies report that alcohol abuse causes health problems, aggravating the conditions of neurological diseases. The role of microRNAs has been highlighted in the literature as biomarkers in various types of diseases, including neurological disorders. Evidence of dysregulation of serum miRNAs related to apoptosis in experimental brain ischemia associated with alcoholism is scarce. We evaluated the gene expression of miRNA-15b and 16 (apoptotic) and miRNA-21, -221 and -222 (anti-apoptotic) in the blood after experimental induction of temporary focal ischemia (90 minutes) by occlusion of the middle cerebral artery in rats, followed by reperfusion for 48 hours, associated with a chronic alcoholism model. Fifty-six rats were randomly divided into seven experimental groups: Control (C); Sham (S); Ischemic (I); Ischemia-reperfusion (IR), Alcoholic (A); Alcoholic and ischemic (I + A); Ischemiareperfusion and Alcoholic (IR+A). The blood samples were collected for gene expression analysis of some serum miRNAs by PCR in real time. The serum expression of miRNA-16 was higher in the IR group compared to C and S groups (P < 0.05) but no association with chronic alcoholism was found. The serum expressions of miRNA-21, -221 and -222 were low in all groups and were not correlated with ischemic injury and/or chronic alcoholism. The serum expressions of miRNA-15b, -21, -221 and -222 were similar among the experimental groups, with no correlation with ischemia, with or without reperfusion, and/or alcoholism. The overexpression of miRNA-16 in the blood of I and IR groups demonstrated a correlation with the ischemic process, mainly after reperfusion for 48 hours, associated or not with alcoholism.Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cirurgia & Anat, Ribeirao Preto, SP, BrazilUniv Marilia, Fac Odontol, Marilia, SP, BrazilUniv Ribeirao Preto, Fac Med, Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilFunpec-editoraUniversidade de São Paulo (USP)Univ MariliaUniversidade Estadual Paulista (Unesp)Novais, P. C.Lizarte Neto, F. S.Cirino, M. L. de A.Porsani, L. B.Silva, J. P. daGula, I. S.Durand, M. de T.Carvalho, C. A. M. deSchimming, B. C. [UNESP]Tazima, M. de F. G. S.Carlotti Jr, C. G.Colli, B. O.Tirapelli, D. P. da C.Tirapelli, L. F.2021-06-25T11:51:51Z2021-06-25T11:51:51Z2020-08-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.4238/gmr18637Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 3, 12 p., 2020.http://hdl.handle.net/11449/20921710.4238/gmr18637WOS:000619540600001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Researchinfo:eu-repo/semantics/openAccess2021-10-23T19:23:38Zoai:repositorio.unesp.br:11449/209217Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
title |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
spellingShingle |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism Novais, P. C. Cerebral Ischemia Apoptosis Alcoholism Morphology miRNA Blood |
title_short |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
title_full |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
title_fullStr |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
title_full_unstemmed |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
title_sort |
Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism |
author |
Novais, P. C. |
author_facet |
Novais, P. C. Lizarte Neto, F. S. Cirino, M. L. de A. Porsani, L. B. Silva, J. P. da Gula, I. S. Durand, M. de T. Carvalho, C. A. M. de Schimming, B. C. [UNESP] Tazima, M. de F. G. S. Carlotti Jr, C. G. Colli, B. O. Tirapelli, D. P. da C. Tirapelli, L. F. |
author_role |
author |
author2 |
Lizarte Neto, F. S. Cirino, M. L. de A. Porsani, L. B. Silva, J. P. da Gula, I. S. Durand, M. de T. Carvalho, C. A. M. de Schimming, B. C. [UNESP] Tazima, M. de F. G. S. Carlotti Jr, C. G. Colli, B. O. Tirapelli, D. P. da C. Tirapelli, L. F. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Marilia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Novais, P. C. Lizarte Neto, F. S. Cirino, M. L. de A. Porsani, L. B. Silva, J. P. da Gula, I. S. Durand, M. de T. Carvalho, C. A. M. de Schimming, B. C. [UNESP] Tazima, M. de F. G. S. Carlotti Jr, C. G. Colli, B. O. Tirapelli, D. P. da C. Tirapelli, L. F. |
dc.subject.por.fl_str_mv |
Cerebral Ischemia Apoptosis Alcoholism Morphology miRNA Blood |
topic |
Cerebral Ischemia Apoptosis Alcoholism Morphology miRNA Blood |
description |
One of the main global causes of mortality is cerebral ischemia. Studies report that alcohol abuse causes health problems, aggravating the conditions of neurological diseases. The role of microRNAs has been highlighted in the literature as biomarkers in various types of diseases, including neurological disorders. Evidence of dysregulation of serum miRNAs related to apoptosis in experimental brain ischemia associated with alcoholism is scarce. We evaluated the gene expression of miRNA-15b and 16 (apoptotic) and miRNA-21, -221 and -222 (anti-apoptotic) in the blood after experimental induction of temporary focal ischemia (90 minutes) by occlusion of the middle cerebral artery in rats, followed by reperfusion for 48 hours, associated with a chronic alcoholism model. Fifty-six rats were randomly divided into seven experimental groups: Control (C); Sham (S); Ischemic (I); Ischemia-reperfusion (IR), Alcoholic (A); Alcoholic and ischemic (I + A); Ischemiareperfusion and Alcoholic (IR+A). The blood samples were collected for gene expression analysis of some serum miRNAs by PCR in real time. The serum expression of miRNA-16 was higher in the IR group compared to C and S groups (P < 0.05) but no association with chronic alcoholism was found. The serum expressions of miRNA-21, -221 and -222 were low in all groups and were not correlated with ischemic injury and/or chronic alcoholism. The serum expressions of miRNA-15b, -21, -221 and -222 were similar among the experimental groups, with no correlation with ischemia, with or without reperfusion, and/or alcoholism. The overexpression of miRNA-16 in the blood of I and IR groups demonstrated a correlation with the ischemic process, mainly after reperfusion for 48 hours, associated or not with alcoholism. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-31 2021-06-25T11:51:51Z 2021-06-25T11:51:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/gmr18637 Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 3, 12 p., 2020. http://hdl.handle.net/11449/209217 10.4238/gmr18637 WOS:000619540600001 |
url |
http://dx.doi.org/10.4238/gmr18637 http://hdl.handle.net/11449/209217 |
identifier_str_mv |
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 3, 12 p., 2020. 10.4238/gmr18637 WOS:000619540600001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790401113882624 |