Proteolytic activity, degradation, and dissolution of primary and permanent teeth
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/ipd.12632 http://hdl.handle.net/11449/197205 |
Resumo: | Background Primary and permanent teeth composition may influence dissolution and degradation rates. Aim To compare the dissolution and degradation of primary and permanent teeth. Design Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (alpha = 0.05). Results Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction inEwas recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD. |
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Proteolytic activity, degradation, and dissolution of primary and permanent teethcollagendentindissolutionenamelproteasesBackground Primary and permanent teeth composition may influence dissolution and degradation rates. Aim To compare the dissolution and degradation of primary and permanent teeth. Design Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (alpha = 0.05). Results Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction inEwas recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.NIDCR NIH HHSCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP, BrazilUniv Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP, Piracicaba, SP, BrazilAugusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA USAUniv Kentucky, Div Pediat Dent, Lexington, KY USASao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Patol, Araraquara, SP, BrazilState Univ Maringa UEM, Dept Dent, Maringa, Parana, BrazilUniv Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USASao Paulo Univ USP, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP, BrazilUniv Ribeirao Preto Unaerp, Dept Physiol & Pathol, Ribeirao Preto, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Patol, Araraquara, SP, BrazilNIDCR NIH HHS: R01 DE05306CAPES: 693711-0FAPESP: 18160-7,FAPESP: 02278-8; 2009FAPESP: 07924-8; 2014FAPESP: 2008FAPESP: 693711-0,FAPESP: 2014FAPESP: 17232-4FAPESP: 2014/17232-4FAPESP: 2009/07924-8FAPESP: 2008/02278-8FAPESP: 2014/18160-7Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Augusta UnivUniv KentuckyUniversidade Estadual de Maringá (UEM)Univ MichiganUniversidade de São Paulo (USP)Univ Ribeirao Preto UnaerpSalles Scheffel, Debora Lopes [UNESP]Cury, Jaime AparecidoAndalo Tenuta, Livia MariaScheffel, Regis HenkePerez, CristinaSoares, Diana Gabriela [UNESP]Basso, Fernanda Goncalves [UNESP]Souza Costa, Carlos Alberto de [UNESP]Pashley, David HenryHebling, Josimeri [UNESP]2020-12-10T20:09:31Z2020-12-10T20:09:31Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article650-659http://dx.doi.org/10.1111/ipd.12632International Journal Of Paediatric Dentistry. Hoboken: Wiley, v. 30, n. 5, p. 650-659, 2020.0960-7439http://hdl.handle.net/11449/19720510.1111/ipd.12632WOS:000561169800016Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Paediatric Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T12:23:58Zoai:repositorio.unesp.br:11449/197205Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:23:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
title |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
spellingShingle |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth Salles Scheffel, Debora Lopes [UNESP] collagen dentin dissolution enamel proteases |
title_short |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
title_full |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
title_fullStr |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
title_full_unstemmed |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
title_sort |
Proteolytic activity, degradation, and dissolution of primary and permanent teeth |
author |
Salles Scheffel, Debora Lopes [UNESP] |
author_facet |
Salles Scheffel, Debora Lopes [UNESP] Cury, Jaime Aparecido Andalo Tenuta, Livia Maria Scheffel, Regis Henke Perez, Cristina Soares, Diana Gabriela [UNESP] Basso, Fernanda Goncalves [UNESP] Souza Costa, Carlos Alberto de [UNESP] Pashley, David Henry Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Cury, Jaime Aparecido Andalo Tenuta, Livia Maria Scheffel, Regis Henke Perez, Cristina Soares, Diana Gabriela [UNESP] Basso, Fernanda Goncalves [UNESP] Souza Costa, Carlos Alberto de [UNESP] Pashley, David Henry Hebling, Josimeri [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Augusta Univ Univ Kentucky Universidade Estadual de Maringá (UEM) Univ Michigan Universidade de São Paulo (USP) Univ Ribeirao Preto Unaerp |
dc.contributor.author.fl_str_mv |
Salles Scheffel, Debora Lopes [UNESP] Cury, Jaime Aparecido Andalo Tenuta, Livia Maria Scheffel, Regis Henke Perez, Cristina Soares, Diana Gabriela [UNESP] Basso, Fernanda Goncalves [UNESP] Souza Costa, Carlos Alberto de [UNESP] Pashley, David Henry Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
collagen dentin dissolution enamel proteases |
topic |
collagen dentin dissolution enamel proteases |
description |
Background Primary and permanent teeth composition may influence dissolution and degradation rates. Aim To compare the dissolution and degradation of primary and permanent teeth. Design Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (alpha = 0.05). Results Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction inEwas recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:09:31Z 2020-12-10T20:09:31Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/ipd.12632 International Journal Of Paediatric Dentistry. Hoboken: Wiley, v. 30, n. 5, p. 650-659, 2020. 0960-7439 http://hdl.handle.net/11449/197205 10.1111/ipd.12632 WOS:000561169800016 |
url |
http://dx.doi.org/10.1111/ipd.12632 http://hdl.handle.net/11449/197205 |
identifier_str_mv |
International Journal Of Paediatric Dentistry. Hoboken: Wiley, v. 30, n. 5, p. 650-659, 2020. 0960-7439 10.1111/ipd.12632 WOS:000561169800016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Paediatric Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
650-659 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964992927170560 |