Sorting and cryopreservation of goat sperm with or without phenolic compounds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4162-11354 http://hdl.handle.net/11449/195362 |
Resumo: | The objectives of this study were to evaluate goat sperm sorting in continuous Percoll (R) density gradients and gamete freezability, in the presence or absence of phenolic antioxidants. For this, semen pools were sorted, frozen, and evaluated. The non-selected group (NSg) presented lower progressive motility (PM), linearity (LIN), straightness (STR), and wobble (WOB) than the selected groups, and straight line velocity (VSL) compared to those with catechin or resveratrol. The amplitude of lateral head displacement (ALH) was higher in NSg, and quercetin reduced the mitochondrial membrane potential (MMP). After thawing, the NSg presented lower PM than the selected groups, VSL and VAP (average path velocity) than the selected group with or without catechin, LIN and WOB than the selected with or without catechin or resveratrol, and STR than the selected with catechin. Moreover, NSg presented higher ALH and BCF than the samples selected with or without catechin. Plasma membrane integrity and intact and living cells were higher in the selected groups, and MMP was lower in the NSg and the selected group with quercetin. Thus, centrifugation in Percoll (R) continuous density gradients is a viable methodology to select goat sperm compatible with the freezing, especially in the presence of catechin or resveratrol. |
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Sorting and cryopreservation of goat sperm with or without phenolic compoundsFlavonoidsoxidative stresssemen freezingsperm selectionstilbenesThe objectives of this study were to evaluate goat sperm sorting in continuous Percoll (R) density gradients and gamete freezability, in the presence or absence of phenolic antioxidants. For this, semen pools were sorted, frozen, and evaluated. The non-selected group (NSg) presented lower progressive motility (PM), linearity (LIN), straightness (STR), and wobble (WOB) than the selected groups, and straight line velocity (VSL) compared to those with catechin or resveratrol. The amplitude of lateral head displacement (ALH) was higher in NSg, and quercetin reduced the mitochondrial membrane potential (MMP). After thawing, the NSg presented lower PM than the selected groups, VSL and VAP (average path velocity) than the selected group with or without catechin, LIN and WOB than the selected with or without catechin or resveratrol, and STR than the selected with catechin. Moreover, NSg presented higher ALH and BCF than the samples selected with or without catechin. Plasma membrane integrity and intact and living cells were higher in the selected groups, and MMP was lower in the NSg and the selected group with quercetin. Thus, centrifugation in Percoll (R) continuous density gradients is a viable methodology to select goat sperm compatible with the freezing, especially in the presence of catechin or resveratrol.Univ Fed Rural Pernambuco, Recife, PE, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilArquivo Brasileiro Medicina Veterinaria ZootecniaUniv Fed Rural PernambucoUniversidade Estadual Paulista (Unesp)Silva, E. C. B.Vieira, J. I. T.Nery, I. H. A.Araujo Silva, R. A. J.Lima, V. F. M. H. [UNESP]Guerra, M. M. P.2020-12-10T17:31:52Z2020-12-10T17:31:52Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article295-304application/pdfhttp://dx.doi.org/10.1590/1678-4162-11354Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 72, n. 2, p. 295-304, 2020.0102-0935http://hdl.handle.net/11449/19536210.1590/1678-4162-11354S0102-09352020000200295WOS:000531604300001S0102-09352020000200295.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro De Medicina Veterinaria E Zootecniainfo:eu-repo/semantics/openAccess2023-10-02T06:08:46Zoai:repositorio.unesp.br:11449/195362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-02T06:08:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
title |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
spellingShingle |
Sorting and cryopreservation of goat sperm with or without phenolic compounds Silva, E. C. B. Flavonoids oxidative stress semen freezing sperm selection stilbenes |
title_short |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
title_full |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
title_fullStr |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
title_full_unstemmed |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
title_sort |
Sorting and cryopreservation of goat sperm with or without phenolic compounds |
author |
Silva, E. C. B. |
author_facet |
Silva, E. C. B. Vieira, J. I. T. Nery, I. H. A. Araujo Silva, R. A. J. Lima, V. F. M. H. [UNESP] Guerra, M. M. P. |
author_role |
author |
author2 |
Vieira, J. I. T. Nery, I. H. A. Araujo Silva, R. A. J. Lima, V. F. M. H. [UNESP] Guerra, M. M. P. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Rural Pernambuco Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, E. C. B. Vieira, J. I. T. Nery, I. H. A. Araujo Silva, R. A. J. Lima, V. F. M. H. [UNESP] Guerra, M. M. P. |
dc.subject.por.fl_str_mv |
Flavonoids oxidative stress semen freezing sperm selection stilbenes |
topic |
Flavonoids oxidative stress semen freezing sperm selection stilbenes |
description |
The objectives of this study were to evaluate goat sperm sorting in continuous Percoll (R) density gradients and gamete freezability, in the presence or absence of phenolic antioxidants. For this, semen pools were sorted, frozen, and evaluated. The non-selected group (NSg) presented lower progressive motility (PM), linearity (LIN), straightness (STR), and wobble (WOB) than the selected groups, and straight line velocity (VSL) compared to those with catechin or resveratrol. The amplitude of lateral head displacement (ALH) was higher in NSg, and quercetin reduced the mitochondrial membrane potential (MMP). After thawing, the NSg presented lower PM than the selected groups, VSL and VAP (average path velocity) than the selected group with or without catechin, LIN and WOB than the selected with or without catechin or resveratrol, and STR than the selected with catechin. Moreover, NSg presented higher ALH and BCF than the samples selected with or without catechin. Plasma membrane integrity and intact and living cells were higher in the selected groups, and MMP was lower in the NSg and the selected group with quercetin. Thus, centrifugation in Percoll (R) continuous density gradients is a viable methodology to select goat sperm compatible with the freezing, especially in the presence of catechin or resveratrol. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:31:52Z 2020-12-10T17:31:52Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-11354 Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 72, n. 2, p. 295-304, 2020. 0102-0935 http://hdl.handle.net/11449/195362 10.1590/1678-4162-11354 S0102-09352020000200295 WOS:000531604300001 S0102-09352020000200295.pdf |
url |
http://dx.doi.org/10.1590/1678-4162-11354 http://hdl.handle.net/11449/195362 |
identifier_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 72, n. 2, p. 295-304, 2020. 0102-0935 10.1590/1678-4162-11354 S0102-09352020000200295 WOS:000531604300001 S0102-09352020000200295.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
295-304 application/pdf |
dc.publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789270679748608 |