A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data

Detalhes bibliográficos
Autor(a) principal: da Silva, Maria Regina Lucas [UNESP]
Data de Publicação: 2018
Outros Autores: Fornazari, Felipe [UNESP], Martins, Thiago Fernandes, Hippólito, Alícia Giolo [UNESP], Rolim, Luna Scarpari [UNESP], Bisca, Jacqueline Muniz [UNESP], Teixeira, Carlos Roberto [UNESP], O’Dwyer, Lucia Helena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00436-018-5903-x
http://hdl.handle.net/11449/176272
Resumo: Hemoparasites are vector-borne parasites that infect wild carnivores worldwide. Since data on hemoparasite infections in Nasua nasua from Brazil are lacking, the aim of this study was to investigate the occurrence of hemoparasites and ectoparasites in N. nasua from different areas of Brazil. Blood samples and ectoparasites from 83 N. nasua were collected in Botucatu, Palmital, and São Paulo municipalities. Samples were screened via microscopy and molecular methods to detect hemoparasites. Tissues from two N. nasua were obtained for histopathological and molecular analyses. All 83 samples were negative for piroplasms on morphological and molecular examination. Thin blood smears of nine animals were positive for Hepatozoon gamonts. The gamonts shared morphological characteristics of Hepatozoon procyonis. Meronts were detected in the liver and spleen tissue of one animal. Twenty-one blood samples and four tissue samples were PCR positive for Hepatozoon sp. The sequences obtained were 97% identical to those of Hepatozoon felis, Hepatozoon ursi, and Hepatozoon sp. Based on searches for similarity and morphology, we identified the sequences as belonging to H. procyonis. This study provides epidemiological data on hemoparasite infections and redescribe H. procyonis based on morphological, morphometrical, and molecular analyses.
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spelling A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular dataCoatisHepatozoon procyonisHistologyMolecular characterizationTick-borne pathogensHemoparasites are vector-borne parasites that infect wild carnivores worldwide. Since data on hemoparasite infections in Nasua nasua from Brazil are lacking, the aim of this study was to investigate the occurrence of hemoparasites and ectoparasites in N. nasua from different areas of Brazil. Blood samples and ectoparasites from 83 N. nasua were collected in Botucatu, Palmital, and São Paulo municipalities. Samples were screened via microscopy and molecular methods to detect hemoparasites. Tissues from two N. nasua were obtained for histopathological and molecular analyses. All 83 samples were negative for piroplasms on morphological and molecular examination. Thin blood smears of nine animals were positive for Hepatozoon gamonts. The gamonts shared morphological characteristics of Hepatozoon procyonis. Meronts were detected in the liver and spleen tissue of one animal. Twenty-one blood samples and four tissue samples were PCR positive for Hepatozoon sp. The sequences obtained were 97% identical to those of Hepatozoon felis, Hepatozoon ursi, and Hepatozoon sp. Based on searches for similarity and morphology, we identified the sequences as belonging to H. procyonis. This study provides epidemiological data on hemoparasite infections and redescribe H. procyonis based on morphological, morphometrical, and molecular analyses.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Instituto de Biociências Campus de Botucatu Departamento de Parasitologia UNESP-Universidade Estadual Paulista, Distrito de Rubião JuniorFaculdade de Medicina Veterinária e Zootecnia UNESP-Univ Estadual Paulista, Campus de Botucatu, Distrito de Rubião JuniorDepartamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia – FMVZ Universidade de São Paulo – USPInstituto de Biociências Campus de Botucatu Departamento de Parasitologia UNESP-Universidade Estadual Paulista, Distrito de Rubião JuniorFaculdade de Medicina Veterinária e Zootecnia UNESP-Univ Estadual Paulista, Campus de Botucatu, Distrito de Rubião JuniorUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)da Silva, Maria Regina Lucas [UNESP]Fornazari, Felipe [UNESP]Martins, Thiago FernandesHippólito, Alícia Giolo [UNESP]Rolim, Luna Scarpari [UNESP]Bisca, Jacqueline Muniz [UNESP]Teixeira, Carlos Roberto [UNESP]O’Dwyer, Lucia Helena [UNESP]2018-12-11T17:19:53Z2018-12-11T17:19:53Z2018-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2159-2169application/pdfhttp://dx.doi.org/10.1007/s00436-018-5903-xParasitology Research, v. 117, n. 7, p. 2159-2169, 2018.1432-19550932-0113http://hdl.handle.net/11449/17627210.1007/s00436-018-5903-x2-s2.0-850464830712-s2.0-85046483071.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Research0,9910,991info:eu-repo/semantics/openAccess2024-01-05T06:22:09Zoai:repositorio.unesp.br:11449/176272Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:22:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
title A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
spellingShingle A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
da Silva, Maria Regina Lucas [UNESP]
Coatis
Hepatozoon procyonis
Histology
Molecular characterization
Tick-borne pathogens
title_short A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
title_full A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
title_fullStr A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
title_full_unstemmed A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
title_sort A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data
author da Silva, Maria Regina Lucas [UNESP]
author_facet da Silva, Maria Regina Lucas [UNESP]
Fornazari, Felipe [UNESP]
Martins, Thiago Fernandes
Hippólito, Alícia Giolo [UNESP]
Rolim, Luna Scarpari [UNESP]
Bisca, Jacqueline Muniz [UNESP]
Teixeira, Carlos Roberto [UNESP]
O’Dwyer, Lucia Helena [UNESP]
author_role author
author2 Fornazari, Felipe [UNESP]
Martins, Thiago Fernandes
Hippólito, Alícia Giolo [UNESP]
Rolim, Luna Scarpari [UNESP]
Bisca, Jacqueline Muniz [UNESP]
Teixeira, Carlos Roberto [UNESP]
O’Dwyer, Lucia Helena [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv da Silva, Maria Regina Lucas [UNESP]
Fornazari, Felipe [UNESP]
Martins, Thiago Fernandes
Hippólito, Alícia Giolo [UNESP]
Rolim, Luna Scarpari [UNESP]
Bisca, Jacqueline Muniz [UNESP]
Teixeira, Carlos Roberto [UNESP]
O’Dwyer, Lucia Helena [UNESP]
dc.subject.por.fl_str_mv Coatis
Hepatozoon procyonis
Histology
Molecular characterization
Tick-borne pathogens
topic Coatis
Hepatozoon procyonis
Histology
Molecular characterization
Tick-borne pathogens
description Hemoparasites are vector-borne parasites that infect wild carnivores worldwide. Since data on hemoparasite infections in Nasua nasua from Brazil are lacking, the aim of this study was to investigate the occurrence of hemoparasites and ectoparasites in N. nasua from different areas of Brazil. Blood samples and ectoparasites from 83 N. nasua were collected in Botucatu, Palmital, and São Paulo municipalities. Samples were screened via microscopy and molecular methods to detect hemoparasites. Tissues from two N. nasua were obtained for histopathological and molecular analyses. All 83 samples were negative for piroplasms on morphological and molecular examination. Thin blood smears of nine animals were positive for Hepatozoon gamonts. The gamonts shared morphological characteristics of Hepatozoon procyonis. Meronts were detected in the liver and spleen tissue of one animal. Twenty-one blood samples and four tissue samples were PCR positive for Hepatozoon sp. The sequences obtained were 97% identical to those of Hepatozoon felis, Hepatozoon ursi, and Hepatozoon sp. Based on searches for similarity and morphology, we identified the sequences as belonging to H. procyonis. This study provides epidemiological data on hemoparasite infections and redescribe H. procyonis based on morphological, morphometrical, and molecular analyses.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:19:53Z
2018-12-11T17:19:53Z
2018-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00436-018-5903-x
Parasitology Research, v. 117, n. 7, p. 2159-2169, 2018.
1432-1955
0932-0113
http://hdl.handle.net/11449/176272
10.1007/s00436-018-5903-x
2-s2.0-85046483071
2-s2.0-85046483071.pdf
url http://dx.doi.org/10.1007/s00436-018-5903-x
http://hdl.handle.net/11449/176272
identifier_str_mv Parasitology Research, v. 117, n. 7, p. 2159-2169, 2018.
1432-1955
0932-0113
10.1007/s00436-018-5903-x
2-s2.0-85046483071
2-s2.0-85046483071.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology Research
0,991
0,991
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2159-2169
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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