Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2020.106469 http://hdl.handle.net/11449/208994 |
Resumo: | Outcomes of short- (6.5 days) and long-term (14.5 days) estrous synchronization for 6.5 d (G-6.5d) or 14.5 d (G-14.5d) and followed by the 4-day or 3-day declining-dose follicle-stimulating hormone superovulatory regimen, respectively, were compared using 16 estrous-cycling Santa Ines ewes. Non-surgical embryo recovery (NSER) procedures were performed 60 d apart starting 6 or 7 d after the onset of estrus; an i.m. injection of estradiol benzoate and of D-cloprostenol at 16 h was followed by an i.v. oxytocin injection administered 20 min before NSER. There was a longer (P < 0.05) period before estrous onset in ewes during the second (September) compared with the first study replicate (July) by approximately 14 h. The NSER could be performed in 11 of 15 ewes that were in estrus, with an average of three viable-embryos/donor and the mean duration of the procedure being 29 min. There were no differences in superovulatory responses between the two groups of ewes, but there were only degenerated embryos in ewes of the G-6.5d group. In summary: i. the duration of progestin-priming and of multiple-dose pFSH treatment had a limited effect on superovulatory responses in estrous-cycling Santa Ines ewes; ii. NSER is a safe and repeatable method of embryo collection in ewes subsequent to superovulation; and iii. duration of the superovulatory treatment regimen may alter the effects of endogenous steroids on oocyte/embryo quality in ewes. |
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Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimensSheepSuperovulationProgestinEstradiolTrans -cervical uterine flushingOutcomes of short- (6.5 days) and long-term (14.5 days) estrous synchronization for 6.5 d (G-6.5d) or 14.5 d (G-14.5d) and followed by the 4-day or 3-day declining-dose follicle-stimulating hormone superovulatory regimen, respectively, were compared using 16 estrous-cycling Santa Ines ewes. Non-surgical embryo recovery (NSER) procedures were performed 60 d apart starting 6 or 7 d after the onset of estrus; an i.m. injection of estradiol benzoate and of D-cloprostenol at 16 h was followed by an i.v. oxytocin injection administered 20 min before NSER. There was a longer (P < 0.05) period before estrous onset in ewes during the second (September) compared with the first study replicate (July) by approximately 14 h. The NSER could be performed in 11 of 15 ewes that were in estrus, with an average of three viable-embryos/donor and the mean duration of the procedure being 29 min. There were no differences in superovulatory responses between the two groups of ewes, but there were only degenerated embryos in ewes of the G-6.5d group. In summary: i. the duration of progestin-priming and of multiple-dose pFSH treatment had a limited effect on superovulatory responses in estrous-cycling Santa Ines ewes; ii. NSER is a safe and repeatable method of embryo collection in ewes subsequent to superovulation; and iii. duration of the superovulatory treatment regimen may alter the effects of endogenous steroids on oocyte/embryo quality in ewes.Embrapa (Brazilian Agricultural Research Corporation)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Agr & Veterinarian Sci, Dept Phatobiol & Vet Theriogenol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilEmbrapa Goats & Sheep Res Ctr, Estr Sobral Groairas,Km 04,CP 145, BR-62010970 Sobral, CE, BrazilVicosa Fed Univ, Dept Anim Sci, Av PH Rolfs S-N, BR-36571000 Vicosa, MG, BrazilUniv Fed Fluminense, Fac Vet, Av Vital Brasil Filho 64, BR-24230340 Niteroi, RJ, BrazilUniv Guelph, Ontario Vet Coll, Dept Biomed Sci, 50 Stone Rd, Guelph, ON N1G 2W1, CanadaSao Paulo State Univ, Sch Agr & Veterinarian Sci, Dept Phatobiol & Vet Theriogenol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilEmbrapa (Brazilian Agricultural Research Corporation): 22.13.06.026.00.03CNPq: 310166/2012-8FAPEMIG: CVZ-PPM 00042-14Elsevier B.V.Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Vicosa Fed UnivUniversidade Federal Fluminense (UFF)Univ GuelphOliveira, M. E. F. [UNESP]Zambrini, F. N.Souza-Fabjan, J. M. G.Bartlewski, P. M.Guimaraes, J. D.Brandao, F. Z.Fonseca, J. F.2021-06-25T11:45:18Z2021-06-25T11:45:18Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.anireprosci.2020.106469Animal Reproduction Science. Amsterdam: Elsevier, v. 217, 9 p., 2020.0378-4320http://hdl.handle.net/11449/20899410.1016/j.anireprosci.2020.106469WOS:000597285700006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T19:23:27Zoai:repositorio.unesp.br:11449/208994Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
title |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
spellingShingle |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens Oliveira, M. E. F. [UNESP] Sheep Superovulation Progestin Estradiol Trans -cervical uterine flushing |
title_short |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
title_full |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
title_fullStr |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
title_full_unstemmed |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
title_sort |
Repeated trans-cervical embryo recoveries in Santa ines ewes subjected to short- or long-term superovulatory treatment regimens |
author |
Oliveira, M. E. F. [UNESP] |
author_facet |
Oliveira, M. E. F. [UNESP] Zambrini, F. N. Souza-Fabjan, J. M. G. Bartlewski, P. M. Guimaraes, J. D. Brandao, F. Z. Fonseca, J. F. |
author_role |
author |
author2 |
Zambrini, F. N. Souza-Fabjan, J. M. G. Bartlewski, P. M. Guimaraes, J. D. Brandao, F. Z. Fonseca, J. F. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Vicosa Fed Univ Universidade Federal Fluminense (UFF) Univ Guelph |
dc.contributor.author.fl_str_mv |
Oliveira, M. E. F. [UNESP] Zambrini, F. N. Souza-Fabjan, J. M. G. Bartlewski, P. M. Guimaraes, J. D. Brandao, F. Z. Fonseca, J. F. |
dc.subject.por.fl_str_mv |
Sheep Superovulation Progestin Estradiol Trans -cervical uterine flushing |
topic |
Sheep Superovulation Progestin Estradiol Trans -cervical uterine flushing |
description |
Outcomes of short- (6.5 days) and long-term (14.5 days) estrous synchronization for 6.5 d (G-6.5d) or 14.5 d (G-14.5d) and followed by the 4-day or 3-day declining-dose follicle-stimulating hormone superovulatory regimen, respectively, were compared using 16 estrous-cycling Santa Ines ewes. Non-surgical embryo recovery (NSER) procedures were performed 60 d apart starting 6 or 7 d after the onset of estrus; an i.m. injection of estradiol benzoate and of D-cloprostenol at 16 h was followed by an i.v. oxytocin injection administered 20 min before NSER. There was a longer (P < 0.05) period before estrous onset in ewes during the second (September) compared with the first study replicate (July) by approximately 14 h. The NSER could be performed in 11 of 15 ewes that were in estrus, with an average of three viable-embryos/donor and the mean duration of the procedure being 29 min. There were no differences in superovulatory responses between the two groups of ewes, but there were only degenerated embryos in ewes of the G-6.5d group. In summary: i. the duration of progestin-priming and of multiple-dose pFSH treatment had a limited effect on superovulatory responses in estrous-cycling Santa Ines ewes; ii. NSER is a safe and repeatable method of embryo collection in ewes subsequent to superovulation; and iii. duration of the superovulatory treatment regimen may alter the effects of endogenous steroids on oocyte/embryo quality in ewes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-01 2021-06-25T11:45:18Z 2021-06-25T11:45:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2020.106469 Animal Reproduction Science. Amsterdam: Elsevier, v. 217, 9 p., 2020. 0378-4320 http://hdl.handle.net/11449/208994 10.1016/j.anireprosci.2020.106469 WOS:000597285700006 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2020.106469 http://hdl.handle.net/11449/208994 |
identifier_str_mv |
Animal Reproduction Science. Amsterdam: Elsevier, v. 217, 9 p., 2020. 0378-4320 10.1016/j.anireprosci.2020.106469 WOS:000597285700006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962468449878016 |