Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2015.07.002 http://hdl.handle.net/11449/158487 |
Resumo: | Follicular wave status at the beginning of exogenous FSH administration is an important contributor to variability in superovulatory responses in ruminants. Studies in ewes have shown a decrease in the number of ovulations when superovulation is initiated in the presence of ostensibly ovulatory-sized ovarian follicles. Hormonal ablation of large antral follicles with the progestin-estradiol (E-2-17 beta) treatment significantly reduces this variability in superovulated anestrous ewes, but the effects of the treatment in cycling ewes have not yet been assessed. Sixteen Rideau Arcott x Polled Dorset ewes (November-December) received either medroxyprogesterone acetate (MAP)-releasing intravaginal sponges (60 mg) or controlled internal drug release (CIDR) devices (containing 300 mg of natural progesterone) for 14 days (Days 0-14), with a single intramuscular injection of 350 mu g of E-2-17 beta on Day 6. The superovulatory treatment consisted of six injections of porcine FSH (Folltropin-V) given twice daily, followed by a bolus GnRH injection (50 mu g intramuscular) on Day 15. There were no differences (P < 0.05) in the ovulatory responses and embryo yields between the two groups of ewes. In both subsets of animals, the next follicular wave emerged similar to 2.5 days after an E-2-17 beta injection (P > 0.05). A decline in maximum follicle size after an E-2-17 beta injection was more abrupt in CIDR- compared with MAP-treated animals, and the ewes pretreated with exogenous progesterone had significantly more 3-mm follicles at the start of the superovulatory treatment. The metabolic clearance rate of exogenous E-2-17 beta appeared to be greater in MAP-treated ewes, but circulating concentrations of porcine FSH failed to increase significantly after each Folltropin-V injection in CIDR-treated animals. The CIDR-treated ewes exceeded (P < 0.05) their MAP-treated counterparts in serum E-2-17 beta concentrations during superovulation. In spite of differences in antral follicle numbers and endocrine profiles between MAP- and CIDR-treated cyclic ewes receiving E-2-17 beta before ovarian superstimulation, there were no differences in superovulatory responses. (C) 2015 Elsevier Inc. All rights reserved. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimenSheepProgestinEstradiolSuperovulationAntral follicleUltrasonographyFollicular wave status at the beginning of exogenous FSH administration is an important contributor to variability in superovulatory responses in ruminants. Studies in ewes have shown a decrease in the number of ovulations when superovulation is initiated in the presence of ostensibly ovulatory-sized ovarian follicles. Hormonal ablation of large antral follicles with the progestin-estradiol (E-2-17 beta) treatment significantly reduces this variability in superovulated anestrous ewes, but the effects of the treatment in cycling ewes have not yet been assessed. Sixteen Rideau Arcott x Polled Dorset ewes (November-December) received either medroxyprogesterone acetate (MAP)-releasing intravaginal sponges (60 mg) or controlled internal drug release (CIDR) devices (containing 300 mg of natural progesterone) for 14 days (Days 0-14), with a single intramuscular injection of 350 mu g of E-2-17 beta on Day 6. The superovulatory treatment consisted of six injections of porcine FSH (Folltropin-V) given twice daily, followed by a bolus GnRH injection (50 mu g intramuscular) on Day 15. There were no differences (P < 0.05) in the ovulatory responses and embryo yields between the two groups of ewes. In both subsets of animals, the next follicular wave emerged similar to 2.5 days after an E-2-17 beta injection (P > 0.05). A decline in maximum follicle size after an E-2-17 beta injection was more abrupt in CIDR- compared with MAP-treated animals, and the ewes pretreated with exogenous progesterone had significantly more 3-mm follicles at the start of the superovulatory treatment. The metabolic clearance rate of exogenous E-2-17 beta appeared to be greater in MAP-treated ewes, but circulating concentrations of porcine FSH failed to increase significantly after each Folltropin-V injection in CIDR-treated animals. The CIDR-treated ewes exceeded (P < 0.05) their MAP-treated counterparts in serum E-2-17 beta concentrations during superovulation. In spite of differences in antral follicle numbers and endocrine profiles between MAP- and CIDR-treated cyclic ewes receiving E-2-17 beta before ovarian superstimulation, there were no differences in superovulatory responses. (C) 2015 Elsevier Inc. All rights reserved.Natural Sciences and Engineering Research Council of CanadaJordan University of Science and TechnologyUniv Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON N1G 2W1, CanadaAgr Univ Krakow, Dept Anim Biotechnol, Krakow, PolandSao Paulo State Univ, Dept Preventat Vet Med & Anim Reprod, Coll Agr & Vet Sci, Sao Paulo, BrazilAgr Univ Krakow, Inst Anim Sci, Krakow, PolandJordan Univ Sci & Technol, Dept Anim Prod, Irbid, JordanSao Paulo State Univ, Dept Preventat Vet Med & Anim Reprod, Coll Agr & Vet Sci, Sao Paulo, BrazilNatural Sciences and Engineering Research Council of Canada: 400301Elsevier B.V.Univ GuelphAgr Univ KrakowUniversidade Estadual Paulista (Unesp)Jordan Univ Sci & TechnolBartlewski, Pawel M.Seaton, PatriciaSzpila, PatrycjaOliveira, Maria E. F. [UNESP]Murawski, MaciejSchwarz, TomaszKridli, Rami T.Zieba, Dorota A.2018-11-26T15:27:51Z2018-11-26T15:27:51Z2015-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1225-1237application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2015.07.002Theriogenology. New York: Elsevier Science Inc, v. 84, n. 7, p. 1225-1237, 2015.0093-691Xhttp://hdl.handle.net/11449/15848710.1016/j.theriogenology.2015.07.002WOS:000361077700021WOS000361077700021.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2023-12-21T06:25:00Zoai:repositorio.unesp.br:11449/158487Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-21T06:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
title |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
spellingShingle |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen Bartlewski, Pawel M. Sheep Progestin Estradiol Superovulation Antral follicle Ultrasonography |
title_short |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
title_full |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
title_fullStr |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
title_full_unstemmed |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
title_sort |
Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17 beta on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovulated in the multiple-dose Folltropin-V (porcine FSH) regimen |
author |
Bartlewski, Pawel M. |
author_facet |
Bartlewski, Pawel M. Seaton, Patricia Szpila, Patrycja Oliveira, Maria E. F. [UNESP] Murawski, Maciej Schwarz, Tomasz Kridli, Rami T. Zieba, Dorota A. |
author_role |
author |
author2 |
Seaton, Patricia Szpila, Patrycja Oliveira, Maria E. F. [UNESP] Murawski, Maciej Schwarz, Tomasz Kridli, Rami T. Zieba, Dorota A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Guelph Agr Univ Krakow Universidade Estadual Paulista (Unesp) Jordan Univ Sci & Technol |
dc.contributor.author.fl_str_mv |
Bartlewski, Pawel M. Seaton, Patricia Szpila, Patrycja Oliveira, Maria E. F. [UNESP] Murawski, Maciej Schwarz, Tomasz Kridli, Rami T. Zieba, Dorota A. |
dc.subject.por.fl_str_mv |
Sheep Progestin Estradiol Superovulation Antral follicle Ultrasonography |
topic |
Sheep Progestin Estradiol Superovulation Antral follicle Ultrasonography |
description |
Follicular wave status at the beginning of exogenous FSH administration is an important contributor to variability in superovulatory responses in ruminants. Studies in ewes have shown a decrease in the number of ovulations when superovulation is initiated in the presence of ostensibly ovulatory-sized ovarian follicles. Hormonal ablation of large antral follicles with the progestin-estradiol (E-2-17 beta) treatment significantly reduces this variability in superovulated anestrous ewes, but the effects of the treatment in cycling ewes have not yet been assessed. Sixteen Rideau Arcott x Polled Dorset ewes (November-December) received either medroxyprogesterone acetate (MAP)-releasing intravaginal sponges (60 mg) or controlled internal drug release (CIDR) devices (containing 300 mg of natural progesterone) for 14 days (Days 0-14), with a single intramuscular injection of 350 mu g of E-2-17 beta on Day 6. The superovulatory treatment consisted of six injections of porcine FSH (Folltropin-V) given twice daily, followed by a bolus GnRH injection (50 mu g intramuscular) on Day 15. There were no differences (P < 0.05) in the ovulatory responses and embryo yields between the two groups of ewes. In both subsets of animals, the next follicular wave emerged similar to 2.5 days after an E-2-17 beta injection (P > 0.05). A decline in maximum follicle size after an E-2-17 beta injection was more abrupt in CIDR- compared with MAP-treated animals, and the ewes pretreated with exogenous progesterone had significantly more 3-mm follicles at the start of the superovulatory treatment. The metabolic clearance rate of exogenous E-2-17 beta appeared to be greater in MAP-treated ewes, but circulating concentrations of porcine FSH failed to increase significantly after each Folltropin-V injection in CIDR-treated animals. The CIDR-treated ewes exceeded (P < 0.05) their MAP-treated counterparts in serum E-2-17 beta concentrations during superovulation. In spite of differences in antral follicle numbers and endocrine profiles between MAP- and CIDR-treated cyclic ewes receiving E-2-17 beta before ovarian superstimulation, there were no differences in superovulatory responses. (C) 2015 Elsevier Inc. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-15 2018-11-26T15:27:51Z 2018-11-26T15:27:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2015.07.002 Theriogenology. New York: Elsevier Science Inc, v. 84, n. 7, p. 1225-1237, 2015. 0093-691X http://hdl.handle.net/11449/158487 10.1016/j.theriogenology.2015.07.002 WOS:000361077700021 WOS000361077700021.pdf |
url |
http://dx.doi.org/10.1016/j.theriogenology.2015.07.002 http://hdl.handle.net/11449/158487 |
identifier_str_mv |
Theriogenology. New York: Elsevier Science Inc, v. 84, n. 7, p. 1225-1237, 2015. 0093-691X 10.1016/j.theriogenology.2015.07.002 WOS:000361077700021 WOS000361077700021.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1225-1237 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962139504246784 |