Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/srep23615 http://hdl.handle.net/11449/158780 |
Resumo: | Dentistry and orthopedics are undergoing a revolution in order to provide more reliable, comfortable and long-lasting implants to patients. Titanium (Ti) and titanium alloys have been used in dental implants and total hip arthroplasty due to their excellent biocompatibility. However, Ti-based implants in human body suffer surface degradation (corrosion and wear) resulting in the release of metallic ions and solid wear debris (mainly titanium dioxide) leading to peri-implant inflammatory reactions. Unfortunately, our current understanding of the biological interactions with titanium dioxide nanoparticles is still very limited. Taking this into consideration, this study focuses on the internalization of titanium dioxide nanoparticles on primary bone cells, exploring the events occurring at the nano-bio interface. For the first time, we report the selective binding of calcium (Ca), phosphorous (P) and proteins from cell culture medium to anatase nanoparticles that are extremely important for nanoparticle internalization and bone cells survival. In the intricate biological environment, anatase nanoparticles form bio-complexes (mixture of proteins and ions) which act as a kind of 'Trojan-horse' internalization by cells. Furthermore, anatase nanoparticles-induced modifications on cell behavior (viability and internalization) could be understand in detail. The results presented in this report can inspire new strategies for the use of titanium dioxide nanoparticles in several regeneration therapies. |
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Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast CellsDentistry and orthopedics are undergoing a revolution in order to provide more reliable, comfortable and long-lasting implants to patients. Titanium (Ti) and titanium alloys have been used in dental implants and total hip arthroplasty due to their excellent biocompatibility. However, Ti-based implants in human body suffer surface degradation (corrosion and wear) resulting in the release of metallic ions and solid wear debris (mainly titanium dioxide) leading to peri-implant inflammatory reactions. Unfortunately, our current understanding of the biological interactions with titanium dioxide nanoparticles is still very limited. Taking this into consideration, this study focuses on the internalization of titanium dioxide nanoparticles on primary bone cells, exploring the events occurring at the nano-bio interface. For the first time, we report the selective binding of calcium (Ca), phosphorous (P) and proteins from cell culture medium to anatase nanoparticles that are extremely important for nanoparticle internalization and bone cells survival. In the intricate biological environment, anatase nanoparticles form bio-complexes (mixture of proteins and ions) which act as a kind of 'Trojan-horse' internalization by cells. Furthermore, anatase nanoparticles-induced modifications on cell behavior (viability and internalization) could be understand in detail. The results presented in this report can inspire new strategies for the use of titanium dioxide nanoparticles in several regeneration therapies.INMETROFINEPFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)NSFNatl Inst Metrol Qual & Technol, Directory Life Sci Appl Metrol, Rio De Janeiro, BrazilUniv Estadual Paulista, Fac Sci, Inst Biomat Tribocorros & Nanomed IBTN, Brazilian Branch, Sao Paulo, BrazilUniv Grande Rio, Postgrad Program Translat Biomed, Duque De Caxias, BrazilUniv Glasgow, Welcome Trust Ctr Mol Parasitol, Glasgow G12 8QQ, Lanark, ScotlandBrazilian Ctr Res Phys Rio de Janeiro, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biomed Sci, Biomineralizat Lab, Rio De Janeiro, BrazilUniv Haute Alsace, Inst Sci Mat Mulhouse, CNRS, UMR7391, Mulhouse, FranceUniv Illinois, Dept Bioengn, Chicago, IL 60607 USAUniv Illinois, Dept Mech & Ind Engn, Chicago, IL 60607 USAFac Med FASE, Ctr Regenerat Med, Petropolis, BrazilUniv Estadual Paulista, Dept Phys, Sao Paulo, BrazilUniv Fed Fluminense, Sch Dent, Niteroi, RJ, BrazilUniv Estadual Paulista, Fac Sci, Inst Biomat Tribocorros & Nanomed IBTN, Brazilian Branch, Sao Paulo, BrazilUniv Estadual Paulista, Dept Phys, Sao Paulo, BrazilINMETRO: 52600.017263/2013NSF: 1350734NSF: 1100806Nature Publishing GroupNatl Inst Metrol Qual & TechnolUniversidade Estadual Paulista (Unesp)Univ Grande RioUniv GlasgowBrazilian Ctr Res Phys Rio de JaneiroUniversidade Federal do Rio de Janeiro (UFRJ)Univ Haute AlsaceUniv IllinoisFac Med FASEUniversidade Federal Fluminense (UFF)Ribeiro, A. R. [UNESP]Gemini-Piperni, S. [UNESP]Travassos, R.Lemgruber, L.Silva, R. C.Rossi, A. L.Farina, M.Anselme, K.Shokuhfar, T. [UNESP]Shahbazian-Yassar, R. [UNESP]Borojevic, R. [UNESP]Rocha, L. A. [UNESP]Werckmann, J. [UNESP]Granjeiro, J. M. [UNESP]2018-11-26T15:29:10Z2018-11-26T15:29:10Z2016-03-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1038/srep23615Scientific Reports. London: Nature Publishing Group, v. 6, 11 p., 2016.2045-2322http://hdl.handle.net/11449/15878010.1038/srep23615WOS:000372920800002WOS000372920800002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2024-01-27T07:01:04Zoai:repositorio.unesp.br:11449/158780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-27T07:01:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
title |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
spellingShingle |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells Ribeiro, A. R. [UNESP] |
title_short |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
title_full |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
title_fullStr |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
title_full_unstemmed |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
title_sort |
Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells |
author |
Ribeiro, A. R. [UNESP] |
author_facet |
Ribeiro, A. R. [UNESP] Gemini-Piperni, S. [UNESP] Travassos, R. Lemgruber, L. Silva, R. C. Rossi, A. L. Farina, M. Anselme, K. Shokuhfar, T. [UNESP] Shahbazian-Yassar, R. [UNESP] Borojevic, R. [UNESP] Rocha, L. A. [UNESP] Werckmann, J. [UNESP] Granjeiro, J. M. [UNESP] |
author_role |
author |
author2 |
Gemini-Piperni, S. [UNESP] Travassos, R. Lemgruber, L. Silva, R. C. Rossi, A. L. Farina, M. Anselme, K. Shokuhfar, T. [UNESP] Shahbazian-Yassar, R. [UNESP] Borojevic, R. [UNESP] Rocha, L. A. [UNESP] Werckmann, J. [UNESP] Granjeiro, J. M. [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Natl Inst Metrol Qual & Technol Universidade Estadual Paulista (Unesp) Univ Grande Rio Univ Glasgow Brazilian Ctr Res Phys Rio de Janeiro Universidade Federal do Rio de Janeiro (UFRJ) Univ Haute Alsace Univ Illinois Fac Med FASE Universidade Federal Fluminense (UFF) |
dc.contributor.author.fl_str_mv |
Ribeiro, A. R. [UNESP] Gemini-Piperni, S. [UNESP] Travassos, R. Lemgruber, L. Silva, R. C. Rossi, A. L. Farina, M. Anselme, K. Shokuhfar, T. [UNESP] Shahbazian-Yassar, R. [UNESP] Borojevic, R. [UNESP] Rocha, L. A. [UNESP] Werckmann, J. [UNESP] Granjeiro, J. M. [UNESP] |
description |
Dentistry and orthopedics are undergoing a revolution in order to provide more reliable, comfortable and long-lasting implants to patients. Titanium (Ti) and titanium alloys have been used in dental implants and total hip arthroplasty due to their excellent biocompatibility. However, Ti-based implants in human body suffer surface degradation (corrosion and wear) resulting in the release of metallic ions and solid wear debris (mainly titanium dioxide) leading to peri-implant inflammatory reactions. Unfortunately, our current understanding of the biological interactions with titanium dioxide nanoparticles is still very limited. Taking this into consideration, this study focuses on the internalization of titanium dioxide nanoparticles on primary bone cells, exploring the events occurring at the nano-bio interface. For the first time, we report the selective binding of calcium (Ca), phosphorous (P) and proteins from cell culture medium to anatase nanoparticles that are extremely important for nanoparticle internalization and bone cells survival. In the intricate biological environment, anatase nanoparticles form bio-complexes (mixture of proteins and ions) which act as a kind of 'Trojan-horse' internalization by cells. Furthermore, anatase nanoparticles-induced modifications on cell behavior (viability and internalization) could be understand in detail. The results presented in this report can inspire new strategies for the use of titanium dioxide nanoparticles in several regeneration therapies. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-29 2018-11-26T15:29:10Z 2018-11-26T15:29:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/srep23615 Scientific Reports. London: Nature Publishing Group, v. 6, 11 p., 2016. 2045-2322 http://hdl.handle.net/11449/158780 10.1038/srep23615 WOS:000372920800002 WOS000372920800002.pdf |
url |
http://dx.doi.org/10.1038/srep23615 http://hdl.handle.net/11449/158780 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 6, 11 p., 2016. 2045-2322 10.1038/srep23615 WOS:000372920800002 WOS000372920800002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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