Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests
Main Author: | |
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Publication Date: | 2013 |
Other Authors: | , |
Format: | Conference object |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://hdl.handle.net/11449/227679 |
Summary: | The serological profiles of 21 female buffaloes vaccinated between 3 to 8 months of age using Brucella abortus strain 19 (S19) were evaluated by rose bengal (RBT), 2-mercaptoethanol (2ME) and complement fixation (CFT) tests. The serum strains were collected in day zero (day of vaccination), 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. No animal showed reaction in day zero. In 15th day, above 95% of animals revealed reaction in all tests. The maximum levels of antibodies detected by the 2ME were found between 15 and 45th days after vaccination. Among 120 and 150 days after vaccination was observed decreased of reactions in all the tests. All the animals presented absence of reactions in CFT, RBT and 2ME tests at 270, 300 and 360 days after vaccination, respectively. Our finding highlighted early response in CFT compared than other conventional agglutination tests. None of animals presented oscillation of titers or reactions in any test after 360 day of study, which enables the use of CFT, RBT and 2ME after this period without interference of antibodies from vaccine origin using S19 between 3 to 8 months in buffalo heifers. |
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Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation testsBrucellosisBuffaloSerodiagnosisStrain 19VaccinationThe serological profiles of 21 female buffaloes vaccinated between 3 to 8 months of age using Brucella abortus strain 19 (S19) were evaluated by rose bengal (RBT), 2-mercaptoethanol (2ME) and complement fixation (CFT) tests. The serum strains were collected in day zero (day of vaccination), 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. No animal showed reaction in day zero. In 15th day, above 95% of animals revealed reaction in all tests. The maximum levels of antibodies detected by the 2ME were found between 15 and 45th days after vaccination. Among 120 and 150 days after vaccination was observed decreased of reactions in all the tests. All the animals presented absence of reactions in CFT, RBT and 2ME tests at 270, 300 and 360 days after vaccination, respectively. Our finding highlighted early response in CFT compared than other conventional agglutination tests. None of animals presented oscillation of titers or reactions in any test after 360 day of study, which enables the use of CFT, RBT and 2ME after this period without interference of antibodies from vaccine origin using S19 between 3 to 8 months in buffalo heifers.Discipline of Animal Production-Course of Technology in Agribusiness, Sao PauloAnimal Production Department School of Veterinary Medicine and Animal Science Univ Estadual Paulista UNESP, 18618-000, Botucatu, Sao PauloSchool of Veterinary Medicine and Animal Science, UNESP Univ Estadual Paulista, Department of Veterinary Hygiene and Public Health Infectious Diseases of Domestic Animals, Botucatu, Sao PauloAnimal Production Department School of Veterinary Medicine and Animal Science Univ Estadual Paulista UNESP, 18618-000, Botucatu, Sao PauloSchool of Veterinary Medicine and Animal Science, UNESP Univ Estadual Paulista, Department of Veterinary Hygiene and Public Health Infectious Diseases of Domestic Animals, Botucatu, Sao PauloDiscipline of Animal Production-Course of Technology in AgribusinessUniversidade Estadual Paulista (UNESP)de Nardi Júnior, GeraldoJorge, André Mendes [UNESP]Ribeiro, Márcio Garcia [UNESP]2022-04-29T07:14:29Z2022-04-29T07:14:29Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1095-1098Buffalo Bulletin, v. 32, n. SPECIAL ISSUE 2, p. 1095-1098, 2013.0125-6726http://hdl.handle.net/11449/2276792-s2.0-84897871495Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBuffalo Bulletininfo:eu-repo/semantics/openAccess2022-04-29T07:14:29Zoai:repositorio.unesp.br:11449/227679Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:14:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
title |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
spellingShingle |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests de Nardi Júnior, Geraldo Brucellosis Buffalo Serodiagnosis Strain 19 Vaccination |
title_short |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
title_full |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
title_fullStr |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
title_full_unstemmed |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
title_sort |
Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose Bengal, 2-mercaptoethanol and complement fixation tests |
author |
de Nardi Júnior, Geraldo |
author_facet |
de Nardi Júnior, Geraldo Jorge, André Mendes [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author_role |
author |
author2 |
Jorge, André Mendes [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Discipline of Animal Production-Course of Technology in Agribusiness Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Nardi Júnior, Geraldo Jorge, André Mendes [UNESP] Ribeiro, Márcio Garcia [UNESP] |
dc.subject.por.fl_str_mv |
Brucellosis Buffalo Serodiagnosis Strain 19 Vaccination |
topic |
Brucellosis Buffalo Serodiagnosis Strain 19 Vaccination |
description |
The serological profiles of 21 female buffaloes vaccinated between 3 to 8 months of age using Brucella abortus strain 19 (S19) were evaluated by rose bengal (RBT), 2-mercaptoethanol (2ME) and complement fixation (CFT) tests. The serum strains were collected in day zero (day of vaccination), 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. No animal showed reaction in day zero. In 15th day, above 95% of animals revealed reaction in all tests. The maximum levels of antibodies detected by the 2ME were found between 15 and 45th days after vaccination. Among 120 and 150 days after vaccination was observed decreased of reactions in all the tests. All the animals presented absence of reactions in CFT, RBT and 2ME tests at 270, 300 and 360 days after vaccination, respectively. Our finding highlighted early response in CFT compared than other conventional agglutination tests. None of animals presented oscillation of titers or reactions in any test after 360 day of study, which enables the use of CFT, RBT and 2ME after this period without interference of antibodies from vaccine origin using S19 between 3 to 8 months in buffalo heifers. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 2022-04-29T07:14:29Z 2022-04-29T07:14:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Buffalo Bulletin, v. 32, n. SPECIAL ISSUE 2, p. 1095-1098, 2013. 0125-6726 http://hdl.handle.net/11449/227679 2-s2.0-84897871495 |
identifier_str_mv |
Buffalo Bulletin, v. 32, n. SPECIAL ISSUE 2, p. 1095-1098, 2013. 0125-6726 2-s2.0-84897871495 |
url |
http://hdl.handle.net/11449/227679 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Buffalo Bulletin |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1095-1098 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789454302183424 |