Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/bab.2085 http://hdl.handle.net/11449/207011 |
Resumo: | Triacylglycerols (TAGs) can be modified to increase the absorption of fatty acids, prevent obesity, and treat fat malabsorption disorders and metabolic diseases. Medium–long–medium (MLM)-type TAGs, which contain medium-chain fatty acids in the sn-1 and sn-3 positions of the glycerol backbone and a long-chain fatty acid in the sn-2 position, show particularly interesting nutritional characteristics. This study aimed to synthesize MLM-type TAGs by enzymatic acidolysis of grape seed oil with medium-chain capric acid (C10:0) in associated packed bed reactors. The reaction was carried out during 120 H, at 45 °C, using lipase from Rhizomucor miehei (Lipozyme® RM IM). The residence time distribution of reagents in the reactor was quantified to evaluate the reactor behavior and to diagnose the existence of preferential paths. The reaction progress was monitored by analyzing TAG composition and, at the steady state (after 48 H of reaction), the incorporation degree achieved a value of 39.91 ± 2.77%. To enhance the capric acid incorporation, an acidolysis reaction in associated packed bed reactors was performed. The results showed a good operational stability of the biocatalyst, revealing values of half-life 209.64 H, 235.63 H of packed bed and associated packed bed reactor, respectively, and a deactivation coefficient 0.0061 H−1. |
id |
UNSP_51be92f82c81a1d285c7b18c34f61293 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/207011 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactorsacidolysisassociated packed bed reactorgrape seed oilstructured lipidsTriacylglycerols (TAGs) can be modified to increase the absorption of fatty acids, prevent obesity, and treat fat malabsorption disorders and metabolic diseases. Medium–long–medium (MLM)-type TAGs, which contain medium-chain fatty acids in the sn-1 and sn-3 positions of the glycerol backbone and a long-chain fatty acid in the sn-2 position, show particularly interesting nutritional characteristics. This study aimed to synthesize MLM-type TAGs by enzymatic acidolysis of grape seed oil with medium-chain capric acid (C10:0) in associated packed bed reactors. The reaction was carried out during 120 H, at 45 °C, using lipase from Rhizomucor miehei (Lipozyme® RM IM). The residence time distribution of reagents in the reactor was quantified to evaluate the reactor behavior and to diagnose the existence of preferential paths. The reaction progress was monitored by analyzing TAG composition and, at the steady state (after 48 H of reaction), the incorporation degree achieved a value of 39.91 ± 2.77%. To enhance the capric acid incorporation, an acidolysis reaction in associated packed bed reactors was performed. The results showed a good operational stability of the biocatalyst, revealing values of half-life 209.64 H, 235.63 H of packed bed and associated packed bed reactor, respectively, and a deactivation coefficient 0.0061 H−1.Department Clinical Analysis School of Pharmaceutical Sciences Sao Paulo State University (UNESP)Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Sao Paulo State University (UNESP)Department Clinical Analysis School of Pharmaceutical Sciences Sao Paulo State University (UNESP)Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Sao Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Cozentino, Izabela de Souza Correia [UNESP]Rodrigues, Marina de Freitas [UNESP]Mazziero, Vitor Teixeira [UNESP]Cerri, Marcel Otávio [UNESP]Cavallini, Daniela Cardoso Umbelino [UNESP]de Paula, Ariela Veloso [UNESP]2021-06-25T10:47:33Z2021-06-25T10:47:33Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/bab.2085Biotechnology and Applied Biochemistry.1470-87440885-4513http://hdl.handle.net/11449/20701110.1002/bab.20852-s2.0-85098050236Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology and Applied Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T16:00:47Zoai:repositorio.unesp.br:11449/207011Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:00:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
title |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
spellingShingle |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors Cozentino, Izabela de Souza Correia [UNESP] acidolysis associated packed bed reactor grape seed oil structured lipids |
title_short |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
title_full |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
title_fullStr |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
title_full_unstemmed |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
title_sort |
Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors |
author |
Cozentino, Izabela de Souza Correia [UNESP] |
author_facet |
Cozentino, Izabela de Souza Correia [UNESP] Rodrigues, Marina de Freitas [UNESP] Mazziero, Vitor Teixeira [UNESP] Cerri, Marcel Otávio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] de Paula, Ariela Veloso [UNESP] |
author_role |
author |
author2 |
Rodrigues, Marina de Freitas [UNESP] Mazziero, Vitor Teixeira [UNESP] Cerri, Marcel Otávio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] de Paula, Ariela Veloso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cozentino, Izabela de Souza Correia [UNESP] Rodrigues, Marina de Freitas [UNESP] Mazziero, Vitor Teixeira [UNESP] Cerri, Marcel Otávio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] de Paula, Ariela Veloso [UNESP] |
dc.subject.por.fl_str_mv |
acidolysis associated packed bed reactor grape seed oil structured lipids |
topic |
acidolysis associated packed bed reactor grape seed oil structured lipids |
description |
Triacylglycerols (TAGs) can be modified to increase the absorption of fatty acids, prevent obesity, and treat fat malabsorption disorders and metabolic diseases. Medium–long–medium (MLM)-type TAGs, which contain medium-chain fatty acids in the sn-1 and sn-3 positions of the glycerol backbone and a long-chain fatty acid in the sn-2 position, show particularly interesting nutritional characteristics. This study aimed to synthesize MLM-type TAGs by enzymatic acidolysis of grape seed oil with medium-chain capric acid (C10:0) in associated packed bed reactors. The reaction was carried out during 120 H, at 45 °C, using lipase from Rhizomucor miehei (Lipozyme® RM IM). The residence time distribution of reagents in the reactor was quantified to evaluate the reactor behavior and to diagnose the existence of preferential paths. The reaction progress was monitored by analyzing TAG composition and, at the steady state (after 48 H of reaction), the incorporation degree achieved a value of 39.91 ± 2.77%. To enhance the capric acid incorporation, an acidolysis reaction in associated packed bed reactors was performed. The results showed a good operational stability of the biocatalyst, revealing values of half-life 209.64 H, 235.63 H of packed bed and associated packed bed reactor, respectively, and a deactivation coefficient 0.0061 H−1. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:47:33Z 2021-06-25T10:47:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/bab.2085 Biotechnology and Applied Biochemistry. 1470-8744 0885-4513 http://hdl.handle.net/11449/207011 10.1002/bab.2085 2-s2.0-85098050236 |
url |
http://dx.doi.org/10.1002/bab.2085 http://hdl.handle.net/11449/207011 |
identifier_str_mv |
Biotechnology and Applied Biochemistry. 1470-8744 0885-4513 10.1002/bab.2085 2-s2.0-85098050236 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biotechnology and Applied Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789398318710784 |