Polarized M2 macrophages in dogs with visceral leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2016.06.032 http://hdl.handle.net/11449/173198 |
Resumo: | The objective of the present study was to analyze the skin (nasal surface and ear regions), lymph nodes (popliteal and pre-scapular), spleen and liver of dogs with visceral leishmaniasis (VL), in order to investigate the relationship between the parasite load measured as DNA copy number of Alpha gene of DNA polymerase of Leishmania infantum by quantitative PCR and the number of M2 macrophages by immunohistochemistry. A set of 29 naturally infected dogs from an endemic area for VL were sampled and another set of six dogs negative for VL and from a non-endemic area were analyzed as the control group (C). The spleen presented the highest number of Leishmania DNA copies, with significant differences between the groups G1 and G2 (with and without skin lesions, respectively). The M2 phenotype immunostaining predominated among the macrophages in granulomas and inflammatory infiltrates of samples from the skin, lymph nodes and spleens examined. The presence of M2 macrophages in dogs from infected group differed significantly from the control group, in all organs analyzed, excepted liver. The highest proportion of M2 macrophages coincided with the highest parasitism loads found in more susceptible organs of VL dogs, even in the skin, considered a more resistant organ, while the liver showed low parasitism load and low immunostaining for M2 macrophages with no significant differences between infected and negative groups. It was concluded that the predominance of M2 phenotype in VL dogs favored the multiplication of Leishmania infantum in organs of dogs that are more susceptible to Leishmania infection, as skin, lymph nodes and spleen. |
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Polarized M2 macrophages in dogs with visceral leishmaniasisDogsLeishmania infantumPhenotype phagocytesThe objective of the present study was to analyze the skin (nasal surface and ear regions), lymph nodes (popliteal and pre-scapular), spleen and liver of dogs with visceral leishmaniasis (VL), in order to investigate the relationship between the parasite load measured as DNA copy number of Alpha gene of DNA polymerase of Leishmania infantum by quantitative PCR and the number of M2 macrophages by immunohistochemistry. A set of 29 naturally infected dogs from an endemic area for VL were sampled and another set of six dogs negative for VL and from a non-endemic area were analyzed as the control group (C). The spleen presented the highest number of Leishmania DNA copies, with significant differences between the groups G1 and G2 (with and without skin lesions, respectively). The M2 phenotype immunostaining predominated among the macrophages in granulomas and inflammatory infiltrates of samples from the skin, lymph nodes and spleens examined. The presence of M2 macrophages in dogs from infected group differed significantly from the control group, in all organs analyzed, excepted liver. The highest proportion of M2 macrophages coincided with the highest parasitism loads found in more susceptible organs of VL dogs, even in the skin, considered a more resistant organ, while the liver showed low parasitism load and low immunostaining for M2 macrophages with no significant differences between infected and negative groups. It was concluded that the predominance of M2 phenotype in VL dogs favored the multiplication of Leishmania infantum in organs of dogs that are more susceptible to Leishmania infection, as skin, lymph nodes and spleen.FCAV/UNESP (Faculdade de Ciências Agrárias and Veterinárias Universidade Estadual Paulista), JaboticabalPostgraduate Veterinary Medicine Program FCAV/UNESP, JaboticabalDepartment of Veterinary Pathology FCAV/UNESP, JaboticabalFCAV/UNESP (Faculdade de Ciências Agrárias and Veterinárias Universidade Estadual Paulista), JaboticabalPostgraduate Veterinary Medicine Program FCAV/UNESP, JaboticabalDepartment of Veterinary Pathology FCAV/UNESP, JaboticabalUniversidade Estadual Paulista (Unesp)Moreira, Pamela Rodrigues Reina [UNESP]Fernando, Filipe Santos [UNESP]Montassier, Hélio José [UNESP]André, Marcos Rogério [UNESP]de Oliveira Vasconcelos, Rosemeri [UNESP]2018-12-11T17:04:06Z2018-12-11T17:04:06Z2016-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article69-73application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2016.06.032Veterinary Parasitology, v. 226, p. 69-73.1873-25500304-4017http://hdl.handle.net/11449/17319810.1016/j.vetpar.2016.06.0322-s2.0-849776398602-s2.0-84977639860.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology1,275info:eu-repo/semantics/openAccess2023-10-19T06:04:54Zoai:repositorio.unesp.br:11449/173198Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-19T06:04:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
title |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
spellingShingle |
Polarized M2 macrophages in dogs with visceral leishmaniasis Moreira, Pamela Rodrigues Reina [UNESP] Dogs Leishmania infantum Phenotype phagocytes |
title_short |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
title_full |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
title_fullStr |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
title_full_unstemmed |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
title_sort |
Polarized M2 macrophages in dogs with visceral leishmaniasis |
author |
Moreira, Pamela Rodrigues Reina [UNESP] |
author_facet |
Moreira, Pamela Rodrigues Reina [UNESP] Fernando, Filipe Santos [UNESP] Montassier, Hélio José [UNESP] André, Marcos Rogério [UNESP] de Oliveira Vasconcelos, Rosemeri [UNESP] |
author_role |
author |
author2 |
Fernando, Filipe Santos [UNESP] Montassier, Hélio José [UNESP] André, Marcos Rogério [UNESP] de Oliveira Vasconcelos, Rosemeri [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moreira, Pamela Rodrigues Reina [UNESP] Fernando, Filipe Santos [UNESP] Montassier, Hélio José [UNESP] André, Marcos Rogério [UNESP] de Oliveira Vasconcelos, Rosemeri [UNESP] |
dc.subject.por.fl_str_mv |
Dogs Leishmania infantum Phenotype phagocytes |
topic |
Dogs Leishmania infantum Phenotype phagocytes |
description |
The objective of the present study was to analyze the skin (nasal surface and ear regions), lymph nodes (popliteal and pre-scapular), spleen and liver of dogs with visceral leishmaniasis (VL), in order to investigate the relationship between the parasite load measured as DNA copy number of Alpha gene of DNA polymerase of Leishmania infantum by quantitative PCR and the number of M2 macrophages by immunohistochemistry. A set of 29 naturally infected dogs from an endemic area for VL were sampled and another set of six dogs negative for VL and from a non-endemic area were analyzed as the control group (C). The spleen presented the highest number of Leishmania DNA copies, with significant differences between the groups G1 and G2 (with and without skin lesions, respectively). The M2 phenotype immunostaining predominated among the macrophages in granulomas and inflammatory infiltrates of samples from the skin, lymph nodes and spleens examined. The presence of M2 macrophages in dogs from infected group differed significantly from the control group, in all organs analyzed, excepted liver. The highest proportion of M2 macrophages coincided with the highest parasitism loads found in more susceptible organs of VL dogs, even in the skin, considered a more resistant organ, while the liver showed low parasitism load and low immunostaining for M2 macrophages with no significant differences between infected and negative groups. It was concluded that the predominance of M2 phenotype in VL dogs favored the multiplication of Leishmania infantum in organs of dogs that are more susceptible to Leishmania infection, as skin, lymph nodes and spleen. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-15 2018-12-11T17:04:06Z 2018-12-11T17:04:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2016.06.032 Veterinary Parasitology, v. 226, p. 69-73. 1873-2550 0304-4017 http://hdl.handle.net/11449/173198 10.1016/j.vetpar.2016.06.032 2-s2.0-84977639860 2-s2.0-84977639860.pdf |
url |
http://dx.doi.org/10.1016/j.vetpar.2016.06.032 http://hdl.handle.net/11449/173198 |
identifier_str_mv |
Veterinary Parasitology, v. 226, p. 69-73. 1873-2550 0304-4017 10.1016/j.vetpar.2016.06.032 2-s2.0-84977639860 2-s2.0-84977639860.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology 1,275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
69-73 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961589071052800 |