Alveolar osseous defect in rat for cell therapy. Preliminary report
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502010000400002 http://hdl.handle.net/11449/14657 |
Resumo: | Purpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm(2) of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 +/- 1.31 mm(2) and the average area of alveolar defect at time point of 4 weeks was 9.03 +/- 1.17 mm(2). The average amount of bone formation at time point of 2 weeks was 5.73 +/- 1.31 mm(2) and the average amount of bone formation at time point of 4 weeks was 19 +/- 1.17 mm(2). Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats. |
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Alveolar osseous defect in rat for cell therapy. Preliminary reportDefeito ósseo alveolar em ratos para terapia celular. Estudo preliminarAlveolar Bone LossTissue TherapyRatsPurpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm(2) of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 +/- 1.31 mm(2) and the average area of alveolar defect at time point of 4 weeks was 9.03 +/- 1.17 mm(2). The average amount of bone formation at time point of 2 weeks was 5.73 +/- 1.31 mm(2) and the average amount of bone formation at time point of 4 weeks was 19 +/- 1.17 mm(2). Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.OBJETIVO: Reproduzir um novo modelo de defeito ósseo alveolar em ratos Wistar que será utilizado para terapia genética e estudos com células tronco. Adicionalmente, outro objetivo do presente estudo foi determinar o pico de regeneração óssea do defeito criado na região alveolar do modelo experimental. MÉTODOS: Os animais foram aleatoriamente divididos em dois grupos de sete animais. Através de uma incisão gengivobucal foi criado um defeito ósseo medindo 28 mm2 de área na região alveolar dos ratos. Os ratos foram sacrificados após duas semanas (n=7) e quatro semanas (n=7) da cirurgia. RESULTADOS: A área média do defeito alveolar após duas semanas de cirurgia foi de 22.27 ± 1.31 mm2 e a área média do defeito alveolar após quatro semanas de cirurgia foi de 9.03 ± 1.17 mm2. A taxa de formação óssea foi de 5.73 ± 1.31 mm2 após duas semanas de cirurgia e de 19 ± 1.17 mm2 após quatro semanas de cirurgia. Foi observada diferença estatisticamente significante na taxa de formação óssea entre o grupo dos animais sacrificados com duas e quatro semanas (p=0.003). CONCLUSÃO: Este estudo demonstrou que a maior taxa de regeneração óssea ocorreu no período entre duas e quatro semanas após a cirurgia de criação do defeito ósseo alveolar, portanto esta observação sugere que o período de tempo de quatro semanas será suficiente para avaliar a capacidade de células tronco em regenerar osso em ratos Wistar com defeito ósseo alveolar.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CEPIDInst Craniofacial & Plast Surg, Campinas, SP, BrazilUniv São Paulo, Dept Genet & Evolut Biol, Inst Biosci, BR-09500900 São Paulo, BrazilState Univ São Paulo, Botucatu, SP, BrazilUniv São Paulo, Div Plast Surg, Dept Surg, BR-09500900 São Paulo, BrazilState Univ São Paulo, Botucatu, SP, BrazilActa Cirúrgica BrasileiraInst Craniofacial & Plast SurgUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Raposo-Amaral, Cassio EduardoKobayashi, Gerson ShigeruAlmeida, Ana BeatrizBueno, Daniela F.Freitas, Fatima Rodrigues de Souza E.Vulcano, Luiz Carlos [UNESP]Passos-Bueno, Maria RitaAlonso, Nivaldo2013-09-30T18:28:52Z2014-05-20T13:42:10Z2013-09-30T18:28:52Z2014-05-20T13:42:10Z2010-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article313-317application/pdfhttp://dx.doi.org/10.1590/S0102-86502010000400002Acta Cirúrgica Brasileira. São Paulo: Acta Cirúrgica Brasileira, v. 25, n. 4, p. 313-317, 2010.0102-8650http://hdl.handle.net/11449/14657S0102-86502010000400002WOS:000280607200001S0102-86502010000400002-en.pdf4065105014625753Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-01-08T06:24:13Zoai:repositorio.unesp.br:11449/14657Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-08T06:24:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alveolar osseous defect in rat for cell therapy. Preliminary report Defeito ósseo alveolar em ratos para terapia celular. Estudo preliminar |
title |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
spellingShingle |
Alveolar osseous defect in rat for cell therapy. Preliminary report Raposo-Amaral, Cassio Eduardo Alveolar Bone Loss Tissue Therapy Rats |
title_short |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_full |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_fullStr |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_full_unstemmed |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_sort |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
author |
Raposo-Amaral, Cassio Eduardo |
author_facet |
Raposo-Amaral, Cassio Eduardo Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. Freitas, Fatima Rodrigues de Souza E. Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
author_role |
author |
author2 |
Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. Freitas, Fatima Rodrigues de Souza E. Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Inst Craniofacial & Plast Surg Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Raposo-Amaral, Cassio Eduardo Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. Freitas, Fatima Rodrigues de Souza E. Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
dc.subject.por.fl_str_mv |
Alveolar Bone Loss Tissue Therapy Rats |
topic |
Alveolar Bone Loss Tissue Therapy Rats |
description |
Purpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm(2) of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 +/- 1.31 mm(2) and the average area of alveolar defect at time point of 4 weeks was 9.03 +/- 1.17 mm(2). The average amount of bone formation at time point of 2 weeks was 5.73 +/- 1.31 mm(2) and the average amount of bone formation at time point of 4 weeks was 19 +/- 1.17 mm(2). Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-01 2013-09-30T18:28:52Z 2013-09-30T18:28:52Z 2014-05-20T13:42:10Z 2014-05-20T13:42:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502010000400002 Acta Cirúrgica Brasileira. São Paulo: Acta Cirúrgica Brasileira, v. 25, n. 4, p. 313-317, 2010. 0102-8650 http://hdl.handle.net/11449/14657 S0102-86502010000400002 WOS:000280607200001 S0102-86502010000400002-en.pdf 4065105014625753 |
url |
http://dx.doi.org/10.1590/S0102-86502010000400002 http://hdl.handle.net/11449/14657 |
identifier_str_mv |
Acta Cirúrgica Brasileira. São Paulo: Acta Cirúrgica Brasileira, v. 25, n. 4, p. 313-317, 2010. 0102-8650 S0102-86502010000400002 WOS:000280607200001 S0102-86502010000400002-en.pdf 4065105014625753 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirúrgica Brasileira 0.933 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
313-317 application/pdf |
dc.publisher.none.fl_str_mv |
Acta Cirúrgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirúrgica Brasileira |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790235311996928 |