Alveolar osseous defect in rat for cell therapy. Preliminary report
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502010000400002 http://hdl.handle.net/11449/219581 |
Resumo: | Purpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm 2 of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm 2 and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm 2. The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm 2 and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm 2. Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003).Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats. |
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Alveolar osseous defect in rat for cell therapy. Preliminary reportAlveolar bone lossRatsTissue therapyPurpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm 2 of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm 2 and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm 2. The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm 2 and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm 2. Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003).Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.Institute of Craniofacial and Plastic Surgery, Campinas-SPDepartment of Genetics and Evolution Biology Institute of Bioscience USP, São PauloVeterinary Medicine State University of São Paulo, Botucatu-SPPlastic Surgery Division Department of Surgery USP, São PauloInstitute of Craniofacial and Plastic SurgeryUniversidade de São Paulo (USP)Raposo-Amaral, Cassio EduardoKobayashi, Gerson ShigeruAlmeida, Ana BeatrizBueno, Daniela F.De Souza E Freitas, Fatima RodriguesVulcano, Luiz CarlosPassos-Bueno, Maria RitaAlonso, Nivaldo2022-04-28T18:56:19Z2022-04-28T18:56:19Z2010-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article313-317http://dx.doi.org/10.1590/S0102-86502010000400002Acta Cirurgica Brasileira, v. 25, n. 4, p. 313-317, 2010.0102-86501678-2674http://hdl.handle.net/11449/21958110.1590/S0102-865020100004000022-s2.0-77956168020Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccess2022-04-28T18:56:19Zoai:repositorio.unesp.br:11449/219581Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:56:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
spellingShingle |
Alveolar osseous defect in rat for cell therapy. Preliminary report Raposo-Amaral, Cassio Eduardo Alveolar bone loss Rats Tissue therapy |
title_short |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_full |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_fullStr |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_full_unstemmed |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
title_sort |
Alveolar osseous defect in rat for cell therapy. Preliminary report |
author |
Raposo-Amaral, Cassio Eduardo |
author_facet |
Raposo-Amaral, Cassio Eduardo Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. De Souza E Freitas, Fatima Rodrigues Vulcano, Luiz Carlos Passos-Bueno, Maria Rita Alonso, Nivaldo |
author_role |
author |
author2 |
Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. De Souza E Freitas, Fatima Rodrigues Vulcano, Luiz Carlos Passos-Bueno, Maria Rita Alonso, Nivaldo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Institute of Craniofacial and Plastic Surgery Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Raposo-Amaral, Cassio Eduardo Kobayashi, Gerson Shigeru Almeida, Ana Beatriz Bueno, Daniela F. De Souza E Freitas, Fatima Rodrigues Vulcano, Luiz Carlos Passos-Bueno, Maria Rita Alonso, Nivaldo |
dc.subject.por.fl_str_mv |
Alveolar bone loss Rats Tissue therapy |
topic |
Alveolar bone loss Rats Tissue therapy |
description |
Purpose: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. Methods: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm 2 of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). Results: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm 2 and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm 2. The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm 2 and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm 2. Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003).Conclusion: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-01 2022-04-28T18:56:19Z 2022-04-28T18:56:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502010000400002 Acta Cirurgica Brasileira, v. 25, n. 4, p. 313-317, 2010. 0102-8650 1678-2674 http://hdl.handle.net/11449/219581 10.1590/S0102-86502010000400002 2-s2.0-77956168020 |
url |
http://dx.doi.org/10.1590/S0102-86502010000400002 http://hdl.handle.net/11449/219581 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 25, n. 4, p. 313-317, 2010. 0102-8650 1678-2674 10.1590/S0102-86502010000400002 2-s2.0-77956168020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
313-317 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790063568879616 |