Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.funbio.2019.08.004 http://hdl.handle.net/11449/189599 |
Resumo: | The in vitro susceptibilities of Candida albicans and Candida tropicalis to Antimicrobial Photodynamic Treatment with aluminum phthalocyanine chloride in nanoemulsion (ClAlPc/NE) were investigated. PS concentration- and fluence-dependent cell survival after APDT were compared before and after unbound extracellular PS had been washed out. The PS uptake and its subcellular localization were also determined. Exposure to light in the absence of the PS and treatment with the PS in the absence of light did not kill the fungi. APDT with ClAlPc/NE resulted in a reduction of five orders of magnitude in viability for C. albicans and between four and five orders of magnitude for C. tropicalis. Washing the cells to remove unbound PS before light exposure did not impair fungal inactivation, suggesting that cell photosensitization was mainly carried out by cell bound ClAlPc. The degree of ClAlPc uptake was dependent on its concentration. Internalization of ClAlPc by C. albicans and C. tropicalis was confirmed by confocal fluorescence microscopy that showed the PS does not penetrate the nucleus and instead accumulates in specific regions of the cytoplasm. Our results show that incorporating the water-insoluble ClAlPc into a nanoemulsion leads to an efficient formulation capable of photoinactivating both Candida species. |
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Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsionAntimicrobial photodynamic therapyAntimicrobial photodynamic treatmentFungal photoinactivationFungal photosensitizationThe in vitro susceptibilities of Candida albicans and Candida tropicalis to Antimicrobial Photodynamic Treatment with aluminum phthalocyanine chloride in nanoemulsion (ClAlPc/NE) were investigated. PS concentration- and fluence-dependent cell survival after APDT were compared before and after unbound extracellular PS had been washed out. The PS uptake and its subcellular localization were also determined. Exposure to light in the absence of the PS and treatment with the PS in the absence of light did not kill the fungi. APDT with ClAlPc/NE resulted in a reduction of five orders of magnitude in viability for C. albicans and between four and five orders of magnitude for C. tropicalis. Washing the cells to remove unbound PS before light exposure did not impair fungal inactivation, suggesting that cell photosensitization was mainly carried out by cell bound ClAlPc. The degree of ClAlPc uptake was dependent on its concentration. Internalization of ClAlPc by C. albicans and C. tropicalis was confirmed by confocal fluorescence microscopy that showed the PS does not penetrate the nucleus and instead accumulates in specific regions of the cytoplasm. Our results show that incorporating the water-insoluble ClAlPc into a nanoemulsion leads to an efficient formulation capable of photoinactivating both Candida species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Ribeirão PretoDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, Ribeirão PretoScholl of Pharmacy and Biomolecular Sciences Liverpool John Moores UniversityDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista – UNESPDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista – UNESPFAPESP: 2012/15204-8FAPESP: 2012/22933-6FAPESP: 2016/11386-5FAPESP: 2018/20571-6CNPq: 307738/2018-3CNPq: 425998/2018-5CAPES: 88881.289327/2018-01Universidade de São Paulo (USP)Liverpool John Moores UniversityUniversidade Estadual Paulista (Unesp)Rodrigues, Gabriela BragaBrancini, Guilherme Thomaz PereiraPinto, Marcelo RodriguesPrimo, Fernando Lucas [UNESP]Wainwright, MarkTedesco, Antonio ClaudioBraga, Gilberto Úbida Leite2019-10-06T16:45:50Z2019-10-06T16:45:50Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.funbio.2019.08.004Fungal Biology.1878-6146http://hdl.handle.net/11449/18959910.1016/j.funbio.2019.08.0042-s2.0-85071717071Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFungal Biologyinfo:eu-repo/semantics/openAccess2021-10-22T21:15:51Zoai:repositorio.unesp.br:11449/189599Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:15:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
title |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
spellingShingle |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion Rodrigues, Gabriela Braga Antimicrobial photodynamic therapy Antimicrobial photodynamic treatment Fungal photoinactivation Fungal photosensitization |
title_short |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
title_full |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
title_fullStr |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
title_full_unstemmed |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
title_sort |
Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion |
author |
Rodrigues, Gabriela Braga |
author_facet |
Rodrigues, Gabriela Braga Brancini, Guilherme Thomaz Pereira Pinto, Marcelo Rodrigues Primo, Fernando Lucas [UNESP] Wainwright, Mark Tedesco, Antonio Claudio Braga, Gilberto Úbida Leite |
author_role |
author |
author2 |
Brancini, Guilherme Thomaz Pereira Pinto, Marcelo Rodrigues Primo, Fernando Lucas [UNESP] Wainwright, Mark Tedesco, Antonio Claudio Braga, Gilberto Úbida Leite |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Liverpool John Moores University Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodrigues, Gabriela Braga Brancini, Guilherme Thomaz Pereira Pinto, Marcelo Rodrigues Primo, Fernando Lucas [UNESP] Wainwright, Mark Tedesco, Antonio Claudio Braga, Gilberto Úbida Leite |
dc.subject.por.fl_str_mv |
Antimicrobial photodynamic therapy Antimicrobial photodynamic treatment Fungal photoinactivation Fungal photosensitization |
topic |
Antimicrobial photodynamic therapy Antimicrobial photodynamic treatment Fungal photoinactivation Fungal photosensitization |
description |
The in vitro susceptibilities of Candida albicans and Candida tropicalis to Antimicrobial Photodynamic Treatment with aluminum phthalocyanine chloride in nanoemulsion (ClAlPc/NE) were investigated. PS concentration- and fluence-dependent cell survival after APDT were compared before and after unbound extracellular PS had been washed out. The PS uptake and its subcellular localization were also determined. Exposure to light in the absence of the PS and treatment with the PS in the absence of light did not kill the fungi. APDT with ClAlPc/NE resulted in a reduction of five orders of magnitude in viability for C. albicans and between four and five orders of magnitude for C. tropicalis. Washing the cells to remove unbound PS before light exposure did not impair fungal inactivation, suggesting that cell photosensitization was mainly carried out by cell bound ClAlPc. The degree of ClAlPc uptake was dependent on its concentration. Internalization of ClAlPc by C. albicans and C. tropicalis was confirmed by confocal fluorescence microscopy that showed the PS does not penetrate the nucleus and instead accumulates in specific regions of the cytoplasm. Our results show that incorporating the water-insoluble ClAlPc into a nanoemulsion leads to an efficient formulation capable of photoinactivating both Candida species. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:45:50Z 2019-10-06T16:45:50Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.funbio.2019.08.004 Fungal Biology. 1878-6146 http://hdl.handle.net/11449/189599 10.1016/j.funbio.2019.08.004 2-s2.0-85071717071 |
url |
http://dx.doi.org/10.1016/j.funbio.2019.08.004 http://hdl.handle.net/11449/189599 |
identifier_str_mv |
Fungal Biology. 1878-6146 10.1016/j.funbio.2019.08.004 2-s2.0-85071717071 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fungal Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789798208897024 |