Antimicrobial sonodynamic and photodynamic therapies against Candida albicans
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2018.1439935 http://hdl.handle.net/11449/179794 |
Resumo: | Candida albicans biofilms exhibit unique characteristics and are highly resistant to antifungal agents. Antimicrobial photodynamic therapy (aPDT) is an alternative treatment limited to treating superficial infections due to the poor light penetration. In this manuscript, the antifungal properties of sonodynamic therapy (SDT) were assessed. SDT uses ultrasound instead of light, enabling the treatment of deeper infections. Planktonic cells and biofilms of C. albicans were treated with aPDT or SDT, in addition to combined aPDT/SDT, with cell survival determined using colony forming units. The total biomass and structural integrity of the biofilms were also investigated. The results demonstrated that while individual aPDT or SDT eradicated suspensions, they had little impact on biofilms. However, combined aPDT/SDT significantly reduced the viability and total biomass of biofilms. Microscopic images revealed that biofilms treated with aPDT/SDT were thinner and comprised mainly of dead cells. These results highlight the potential of combined aPDT/SDT for the inactivation of C. albicans biofilms. |
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Antimicrobial sonodynamic and photodynamic therapies against Candida albicansanti-fungalantimicrobialbiofilmsCandida albicansphotodynamic therapysonodynamic therapyCandida albicans biofilms exhibit unique characteristics and are highly resistant to antifungal agents. Antimicrobial photodynamic therapy (aPDT) is an alternative treatment limited to treating superficial infections due to the poor light penetration. In this manuscript, the antifungal properties of sonodynamic therapy (SDT) were assessed. SDT uses ultrasound instead of light, enabling the treatment of deeper infections. Planktonic cells and biofilms of C. albicans were treated with aPDT or SDT, in addition to combined aPDT/SDT, with cell survival determined using colony forming units. The total biomass and structural integrity of the biofilms were also investigated. The results demonstrated that while individual aPDT or SDT eradicated suspensions, they had little impact on biofilms. However, combined aPDT/SDT significantly reduced the viability and total biomass of biofilms. Microscopic images revealed that biofilms treated with aPDT/SDT were thinner and comprised mainly of dead cells. These results highlight the potential of combined aPDT/SDT for the inactivation of C. albicans biofilms.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)School of Pharmacy and Pharmaceutical Sciences Faculty of Life & Health Sciences Ulster UniversityDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)FAPESP: 2014/01262-1FAPESP: 2016/00190-2Universidade Estadual Paulista (Unesp)Ulster UniversityAlves, Fernanda [UNESP]Pavarina, Ana Cláudia [UNESP]Mima, Ewerton Garcia de Oliveira [UNESP]McHale, Anthony P.Callan, John Francis2018-12-11T17:36:47Z2018-12-11T17:36:47Z2018-04-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article357-367application/pdfhttp://dx.doi.org/10.1080/08927014.2018.1439935Biofouling, v. 34, n. 4, p. 357-367, 2018.1029-24540892-7014http://hdl.handle.net/11449/17979410.1080/08927014.2018.14399352-s2.0-850457497312-s2.0-85045749731.pdf62599858990694980000-0002-9575-7625Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2023-10-31T06:10:23Zoai:repositorio.unesp.br:11449/179794Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-31T06:10:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
title |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
spellingShingle |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans Alves, Fernanda [UNESP] anti-fungal antimicrobial biofilms Candida albicans photodynamic therapy sonodynamic therapy |
title_short |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
title_full |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
title_fullStr |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
title_full_unstemmed |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
title_sort |
Antimicrobial sonodynamic and photodynamic therapies against Candida albicans |
author |
Alves, Fernanda [UNESP] |
author_facet |
Alves, Fernanda [UNESP] Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] McHale, Anthony P. Callan, John Francis |
author_role |
author |
author2 |
Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] McHale, Anthony P. Callan, John Francis |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ulster University |
dc.contributor.author.fl_str_mv |
Alves, Fernanda [UNESP] Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] McHale, Anthony P. Callan, John Francis |
dc.subject.por.fl_str_mv |
anti-fungal antimicrobial biofilms Candida albicans photodynamic therapy sonodynamic therapy |
topic |
anti-fungal antimicrobial biofilms Candida albicans photodynamic therapy sonodynamic therapy |
description |
Candida albicans biofilms exhibit unique characteristics and are highly resistant to antifungal agents. Antimicrobial photodynamic therapy (aPDT) is an alternative treatment limited to treating superficial infections due to the poor light penetration. In this manuscript, the antifungal properties of sonodynamic therapy (SDT) were assessed. SDT uses ultrasound instead of light, enabling the treatment of deeper infections. Planktonic cells and biofilms of C. albicans were treated with aPDT or SDT, in addition to combined aPDT/SDT, with cell survival determined using colony forming units. The total biomass and structural integrity of the biofilms were also investigated. The results demonstrated that while individual aPDT or SDT eradicated suspensions, they had little impact on biofilms. However, combined aPDT/SDT significantly reduced the viability and total biomass of biofilms. Microscopic images revealed that biofilms treated with aPDT/SDT were thinner and comprised mainly of dead cells. These results highlight the potential of combined aPDT/SDT for the inactivation of C. albicans biofilms. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:36:47Z 2018-12-11T17:36:47Z 2018-04-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2018.1439935 Biofouling, v. 34, n. 4, p. 357-367, 2018. 1029-2454 0892-7014 http://hdl.handle.net/11449/179794 10.1080/08927014.2018.1439935 2-s2.0-85045749731 2-s2.0-85045749731.pdf 6259985899069498 0000-0002-9575-7625 |
url |
http://dx.doi.org/10.1080/08927014.2018.1439935 http://hdl.handle.net/11449/179794 |
identifier_str_mv |
Biofouling, v. 34, n. 4, p. 357-367, 2018. 1029-2454 0892-7014 10.1080/08927014.2018.1439935 2-s2.0-85045749731 2-s2.0-85045749731.pdf 6259985899069498 0000-0002-9575-7625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
357-367 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961699351887872 |