Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds
Main Author: | |
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Publication Date: | 2023 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng por |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/1678-4162-12747 http://hdl.handle.net/11449/246841 |
Summary: | Porcine hemoplasmosis is characterized as a geographically cosmopolitan disease caused by Mycoplasma suis and Mycoplasma parvum. Asymptomatic pigs are considered the focus of hemoplasmosis because they are carriers and reservoirs to new infections. This study aimed to determine the molecular occurrence of porcine hemoplasmas (PH) in the production cycle of technified farrow-to-finished swine herds. For this purpose, 20 swine herds were evaluated, where 501 whole blood samples were collected for qPCR and phylogenetic analyses for hemoplasmas. The epidemiological analysis was performed for the entire population and per the growth stage. The total prevalence for PH was 31.93% (161/501); 95% (19/20) of sampled herds were positive. The occurrence of PH by swine growth stages was nursery (30.47%), growing (31.29%), finishing (26.18%), and slaughter (40.25%). The quantification cycles (Cq) ranged from 3.18-39.56 and the number of PH 16S rRNA copies per μL of DNA ranged from 5,57 x10-2 to 2.23 x1010. Sequencing and phylogenetic analysis of five selected samples showed 100% identity with M. parvum strain Indiana and two M. parvum sequences from Brazil/Goiás. This is the first report on PH in technified herds in Southeastern Brazil by growth stages. |
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Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds[Prevalência molecular do Mycoplasma parvum em granjas de suínos tecnificados de ciclo completo]bacteremiacarriergrowth stagesMycoplasma parvumPorcine hemoplasmosis is characterized as a geographically cosmopolitan disease caused by Mycoplasma suis and Mycoplasma parvum. Asymptomatic pigs are considered the focus of hemoplasmosis because they are carriers and reservoirs to new infections. This study aimed to determine the molecular occurrence of porcine hemoplasmas (PH) in the production cycle of technified farrow-to-finished swine herds. For this purpose, 20 swine herds were evaluated, where 501 whole blood samples were collected for qPCR and phylogenetic analyses for hemoplasmas. The epidemiological analysis was performed for the entire population and per the growth stage. The total prevalence for PH was 31.93% (161/501); 95% (19/20) of sampled herds were positive. The occurrence of PH by swine growth stages was nursery (30.47%), growing (31.29%), finishing (26.18%), and slaughter (40.25%). The quantification cycles (Cq) ranged from 3.18-39.56 and the number of PH 16S rRNA copies per μL of DNA ranged from 5,57 x10-2 to 2.23 x1010. Sequencing and phylogenetic analysis of five selected samples showed 100% identity with M. parvum strain Indiana and two M. parvum sequences from Brazil/Goiás. This is the first report on PH in technified herds in Southeastern Brazil by growth stages.Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, SPUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, SPUniversidade Estadual Paulista (UNESP)Cruz, N. R.N. [UNESP]André, M. R. [UNESP]Baraldi, T. G. [UNESP]Mathias, L. A. [UNESP]Braz, L. A.N. [UNESP]Oliveira, L. G. [UNESP]Santana, A. E. [UNESP]2023-07-29T12:51:57Z2023-07-29T12:51:57Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article61-70http://dx.doi.org/10.1590/1678-4162-12747Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 75, n. 1, p. 61-70, 2023.1678-41620102-0935http://hdl.handle.net/11449/24684110.1590/1678-4162-127472-s2.0-85148243596Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporArquivo Brasileiro de Medicina Veterinaria e Zootecniainfo:eu-repo/semantics/openAccess2023-07-29T12:51:57Zoai:repositorio.unesp.br:11449/246841Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:51:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds [Prevalência molecular do Mycoplasma parvum em granjas de suínos tecnificados de ciclo completo] |
title |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
spellingShingle |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds Cruz, N. R.N. [UNESP] bacteremia carrier growth stages Mycoplasma parvum |
title_short |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
title_full |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
title_fullStr |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
title_full_unstemmed |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
title_sort |
Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds |
author |
Cruz, N. R.N. [UNESP] |
author_facet |
Cruz, N. R.N. [UNESP] André, M. R. [UNESP] Baraldi, T. G. [UNESP] Mathias, L. A. [UNESP] Braz, L. A.N. [UNESP] Oliveira, L. G. [UNESP] Santana, A. E. [UNESP] |
author_role |
author |
author2 |
André, M. R. [UNESP] Baraldi, T. G. [UNESP] Mathias, L. A. [UNESP] Braz, L. A.N. [UNESP] Oliveira, L. G. [UNESP] Santana, A. E. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Cruz, N. R.N. [UNESP] André, M. R. [UNESP] Baraldi, T. G. [UNESP] Mathias, L. A. [UNESP] Braz, L. A.N. [UNESP] Oliveira, L. G. [UNESP] Santana, A. E. [UNESP] |
dc.subject.por.fl_str_mv |
bacteremia carrier growth stages Mycoplasma parvum |
topic |
bacteremia carrier growth stages Mycoplasma parvum |
description |
Porcine hemoplasmosis is characterized as a geographically cosmopolitan disease caused by Mycoplasma suis and Mycoplasma parvum. Asymptomatic pigs are considered the focus of hemoplasmosis because they are carriers and reservoirs to new infections. This study aimed to determine the molecular occurrence of porcine hemoplasmas (PH) in the production cycle of technified farrow-to-finished swine herds. For this purpose, 20 swine herds were evaluated, where 501 whole blood samples were collected for qPCR and phylogenetic analyses for hemoplasmas. The epidemiological analysis was performed for the entire population and per the growth stage. The total prevalence for PH was 31.93% (161/501); 95% (19/20) of sampled herds were positive. The occurrence of PH by swine growth stages was nursery (30.47%), growing (31.29%), finishing (26.18%), and slaughter (40.25%). The quantification cycles (Cq) ranged from 3.18-39.56 and the number of PH 16S rRNA copies per μL of DNA ranged from 5,57 x10-2 to 2.23 x1010. Sequencing and phylogenetic analysis of five selected samples showed 100% identity with M. parvum strain Indiana and two M. parvum sequences from Brazil/Goiás. This is the first report on PH in technified herds in Southeastern Brazil by growth stages. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:51:57Z 2023-07-29T12:51:57Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-12747 Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 75, n. 1, p. 61-70, 2023. 1678-4162 0102-0935 http://hdl.handle.net/11449/246841 10.1590/1678-4162-12747 2-s2.0-85148243596 |
url |
http://dx.doi.org/10.1590/1678-4162-12747 http://hdl.handle.net/11449/246841 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 75, n. 1, p. 61-70, 2023. 1678-4162 0102-0935 10.1590/1678-4162-12747 2-s2.0-85148243596 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
61-70 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964863597903872 |