Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765202220200092 http://hdl.handle.net/11449/240883 |
Resumo: | Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag- NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics. |
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Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)Ag-NORsC-BandsLeiuperinaePcP190satDNAIntending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag- NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics.Universidad Nacional de Misiones Facultad de Ciencias Exactas Químicas y Naturales Instituto de Biología Subtropical (CONICET-UNaM) Laboratorio de Genética Evolutiva, Félix de Azara 1552, MisionesUniversidad Nacional de Misiones Facultad de Ciencias Exactas Químicas y Naturales, Félix de Azara 1552, MisionesMuseo Nacional de Historia Natural Sección Herpetología, Miguelete 1825Universidade Estadual de Campinas Laboratório de Estudos Cromossômicos Departamento de Biologia Estrutural e Funcional, Bertrand Russell, s/n, SPUniversidade Estadual Paulista Instituto de Biociências Centro de Aquicultura e Laboratório de Herpetologia Departamento de Zoologia, Avenida 24A, Bela Vista, SPUniversidade Estadual Paulista Instituto de Biociências Centro de Aquicultura e Laboratório de Herpetologia Departamento de Zoologia, Avenida 24A, Bela Vista, SPLaboratorio de Genética EvolutivaFacultad de Ciencias Exactas Químicas y NaturalesSección HerpetologíaUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Ferro, Juan M.Taffarel, AlbertoTomatis, CristianBorteiro, ClaudioKolenc, FranciscoGatto, Kaleb P. [UNESP]Lourenço, Luciana B.Baldo, Diego2023-03-01T20:36:55Z2023-03-01T20:36:55Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0001-3765202220200092Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022.1678-26900001-3765http://hdl.handle.net/11449/24088310.1590/0001-37652022202000922-s2.0-85128794347Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Cienciasinfo:eu-repo/semantics/openAccess2024-04-09T15:29:58Zoai:repositorio.unesp.br:11449/240883Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:29:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
spellingShingle |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) Ferro, Juan M. Ag-NORs C-Bands Leiuperinae PcP190 satDNA |
title_short |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_full |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_fullStr |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_full_unstemmed |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_sort |
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
author |
Ferro, Juan M. |
author_facet |
Ferro, Juan M. Taffarel, Alberto Tomatis, Cristian Borteiro, Claudio Kolenc, Francisco Gatto, Kaleb P. [UNESP] Lourenço, Luciana B. Baldo, Diego |
author_role |
author |
author2 |
Taffarel, Alberto Tomatis, Cristian Borteiro, Claudio Kolenc, Francisco Gatto, Kaleb P. [UNESP] Lourenço, Luciana B. Baldo, Diego |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Laboratorio de Genética Evolutiva Facultad de Ciencias Exactas Químicas y Naturales Sección Herpetología Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Ferro, Juan M. Taffarel, Alberto Tomatis, Cristian Borteiro, Claudio Kolenc, Francisco Gatto, Kaleb P. [UNESP] Lourenço, Luciana B. Baldo, Diego |
dc.subject.por.fl_str_mv |
Ag-NORs C-Bands Leiuperinae PcP190 satDNA |
topic |
Ag-NORs C-Bands Leiuperinae PcP190 satDNA |
description |
Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag- NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T20:36:55Z 2023-03-01T20:36:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765202220200092 Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022. 1678-2690 0001-3765 http://hdl.handle.net/11449/240883 10.1590/0001-3765202220200092 2-s2.0-85128794347 |
url |
http://dx.doi.org/10.1590/0001-3765202220200092 http://hdl.handle.net/11449/240883 |
identifier_str_mv |
Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022. 1678-2690 0001-3765 10.1590/0001-3765202220200092 2-s2.0-85128794347 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciencias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789758197334016 |