In vivo selection for Haemonchus contortus resistance to monepantel
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0022149X19000221 http://hdl.handle.net/11449/197928 |
Resumo: | Gastrointestinal nematodes significantly affect the ovine industry, and Haemonchus contortus is considered the most pathogenic parasite in tropical regions. This situation is aggravated when the main strategy to control worms fails because of the genetic resistance that parasites acquire against anthelmintics. Aiming to anticipate the events involved in anthelmintic resistance, we induced monepantel resistance in H. Contortus by in vivo subdosing of sheep hosts. Four successive passages of a monepantel-susceptible H. Contortus isolate in Santa Ines or Ile de France sheep hosts resulted in three monepantel-resistant (efficacy varying from 0 to 58.5%) H. Contortus isolates. Sheep hosts were treated from 0.075 mg/kg to the therapeutic dose of 2.5 mg/kg of monepantel in 19-26 rounds of selection for 112-133 weeks. Success in inducing H. Contortus resistance to monepantel may have been affected by worm burden and by host-parasite interactions, including a possible effect of the breed of sheep hosts. We conclude that subdosing of sheep, although time-consuming, is an efficient in vivo strategy for the induction of monepantel resistance in H. Contortus. The resistant parasites can be used in further studies to elucidate the genetic and biochemical events involved in the acquisition of anthelmintic resistance. |
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In vivo selection for Haemonchus contortus resistance to monepantelgastrointestinal nematodeshost-parasite interactionInduction of anthelmintic resistancelow-dose selectionGastrointestinal nematodes significantly affect the ovine industry, and Haemonchus contortus is considered the most pathogenic parasite in tropical regions. This situation is aggravated when the main strategy to control worms fails because of the genetic resistance that parasites acquire against anthelmintics. Aiming to anticipate the events involved in anthelmintic resistance, we induced monepantel resistance in H. Contortus by in vivo subdosing of sheep hosts. Four successive passages of a monepantel-susceptible H. Contortus isolate in Santa Ines or Ile de France sheep hosts resulted in three monepantel-resistant (efficacy varying from 0 to 58.5%) H. Contortus isolates. Sheep hosts were treated from 0.075 mg/kg to the therapeutic dose of 2.5 mg/kg of monepantel in 19-26 rounds of selection for 112-133 weeks. Success in inducing H. Contortus resistance to monepantel may have been affected by worm burden and by host-parasite interactions, including a possible effect of the breed of sheep hosts. We conclude that subdosing of sheep, although time-consuming, is an efficient in vivo strategy for the induction of monepantel resistance in H. Contortus. The resistant parasites can be used in further studies to elucidate the genetic and biochemical events involved in the acquisition of anthelmintic resistance.Embrapa Pecuária Sudeste Rodovia Washington Luiz, Km 234, Fazenda CanchimCentro Universitário Central Paulista Rua Miguel Petroni, 5111, CEP 13563-470Universidade Federal de São Carlos Rodovia Washington Luiz, Km 235, CEP 13566-905Embrapa Pecuária sul Rodovia BR-153, Km 632, 9, Vila Industrial, CEP 96401-970, Bagé, RSUNESP Instituto de Biociências, Rua Professor Doutor Antônio Celso Wagner Zanin, 250UNESP Instituto de Biociências, Rua Professor Doutor Antônio Celso Wagner Zanin, 250Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Rua Miguel PetroniUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Niciura, S. C.M.Cruvinel, G. G.Moraes, C. V.Chagas, A. C.S.Esteves, S. N.Benavides, M. V.Amarante, A. F.T. [UNESP]2020-12-12T00:54:17Z2020-12-12T00:54:17Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S0022149X19000221Journal of Helminthology, v. 94.1475-26970022-149Xhttp://hdl.handle.net/11449/19792810.1017/S0022149X190002212-s2.0-85070969428Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Helminthologyinfo:eu-repo/semantics/openAccess2021-10-23T07:07:39Zoai:repositorio.unesp.br:11449/197928Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T07:07:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vivo selection for Haemonchus contortus resistance to monepantel |
title |
In vivo selection for Haemonchus contortus resistance to monepantel |
spellingShingle |
In vivo selection for Haemonchus contortus resistance to monepantel Niciura, S. C.M. gastrointestinal nematodes host-parasite interaction Induction of anthelmintic resistance low-dose selection |
title_short |
In vivo selection for Haemonchus contortus resistance to monepantel |
title_full |
In vivo selection for Haemonchus contortus resistance to monepantel |
title_fullStr |
In vivo selection for Haemonchus contortus resistance to monepantel |
title_full_unstemmed |
In vivo selection for Haemonchus contortus resistance to monepantel |
title_sort |
In vivo selection for Haemonchus contortus resistance to monepantel |
author |
Niciura, S. C.M. |
author_facet |
Niciura, S. C.M. Cruvinel, G. G. Moraes, C. V. Chagas, A. C.S. Esteves, S. N. Benavides, M. V. Amarante, A. F.T. [UNESP] |
author_role |
author |
author2 |
Cruvinel, G. G. Moraes, C. V. Chagas, A. C.S. Esteves, S. N. Benavides, M. V. Amarante, A. F.T. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Rua Miguel Petroni Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Niciura, S. C.M. Cruvinel, G. G. Moraes, C. V. Chagas, A. C.S. Esteves, S. N. Benavides, M. V. Amarante, A. F.T. [UNESP] |
dc.subject.por.fl_str_mv |
gastrointestinal nematodes host-parasite interaction Induction of anthelmintic resistance low-dose selection |
topic |
gastrointestinal nematodes host-parasite interaction Induction of anthelmintic resistance low-dose selection |
description |
Gastrointestinal nematodes significantly affect the ovine industry, and Haemonchus contortus is considered the most pathogenic parasite in tropical regions. This situation is aggravated when the main strategy to control worms fails because of the genetic resistance that parasites acquire against anthelmintics. Aiming to anticipate the events involved in anthelmintic resistance, we induced monepantel resistance in H. Contortus by in vivo subdosing of sheep hosts. Four successive passages of a monepantel-susceptible H. Contortus isolate in Santa Ines or Ile de France sheep hosts resulted in three monepantel-resistant (efficacy varying from 0 to 58.5%) H. Contortus isolates. Sheep hosts were treated from 0.075 mg/kg to the therapeutic dose of 2.5 mg/kg of monepantel in 19-26 rounds of selection for 112-133 weeks. Success in inducing H. Contortus resistance to monepantel may have been affected by worm burden and by host-parasite interactions, including a possible effect of the breed of sheep hosts. We conclude that subdosing of sheep, although time-consuming, is an efficient in vivo strategy for the induction of monepantel resistance in H. Contortus. The resistant parasites can be used in further studies to elucidate the genetic and biochemical events involved in the acquisition of anthelmintic resistance. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T00:54:17Z 2020-12-12T00:54:17Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0022149X19000221 Journal of Helminthology, v. 94. 1475-2697 0022-149X http://hdl.handle.net/11449/197928 10.1017/S0022149X19000221 2-s2.0-85070969428 |
url |
http://dx.doi.org/10.1017/S0022149X19000221 http://hdl.handle.net/11449/197928 |
identifier_str_mv |
Journal of Helminthology, v. 94. 1475-2697 0022-149X 10.1017/S0022149X19000221 2-s2.0-85070969428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Helminthology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962034450563072 |