Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0103-64402012000500013 http://hdl.handle.net/11449/73540 |
Resumo: | The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (107 cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions. |
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Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viabilityAcrylic resinsBiofilmsBrushingCandida albicansDenture cleansersDisinfectantsOral hygieneThe adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (107 cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.Department of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, São PauloDivision of Removable Prosthodontics Dental School University of Athens, AthensDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, São PauloUniversidade Estadual Paulista (Unesp)University of AthensPellizzaro, Delise [UNESP]Polyzois, GregoryMachado, Ana Lucia [UNESP]Giampaolo, Eunice Teresinha [UNESP]Sanitá, Paula Volpato [UNESP]Vergani, Carlos Eduardo [UNESP]2014-05-27T11:26:57Z2014-05-27T11:26:57Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article547-554application/pdfhttp://dx.doi.org/10.1590/S0103-64402012000500013Brazilian Dental Journal, v. 23, n. 5, p. 547-554, 2012.0103-64401806-4760http://hdl.handle.net/11449/7354010.1590/S0103-64402012000500013S0103-644020120005000132-s2.0-848724006942-s2.0-84872400694.pdf8000248781842587573712733424854930031305224278200000-0002-7375-4714Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-10-26T06:10:49Zoai:repositorio.unesp.br:11449/73540Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:10:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
spellingShingle |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability Pellizzaro, Delise [UNESP] Acrylic resins Biofilms Brushing Candida albicans Denture cleansers Disinfectants Oral hygiene |
title_short |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_full |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_fullStr |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_full_unstemmed |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_sort |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
author |
Pellizzaro, Delise [UNESP] |
author_facet |
Pellizzaro, Delise [UNESP] Polyzois, Gregory Machado, Ana Lucia [UNESP] Giampaolo, Eunice Teresinha [UNESP] Sanitá, Paula Volpato [UNESP] Vergani, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
Polyzois, Gregory Machado, Ana Lucia [UNESP] Giampaolo, Eunice Teresinha [UNESP] Sanitá, Paula Volpato [UNESP] Vergani, Carlos Eduardo [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Athens |
dc.contributor.author.fl_str_mv |
Pellizzaro, Delise [UNESP] Polyzois, Gregory Machado, Ana Lucia [UNESP] Giampaolo, Eunice Teresinha [UNESP] Sanitá, Paula Volpato [UNESP] Vergani, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Acrylic resins Biofilms Brushing Candida albicans Denture cleansers Disinfectants Oral hygiene |
topic |
Acrylic resins Biofilms Brushing Candida albicans Denture cleansers Disinfectants Oral hygiene |
description |
The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (107 cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2014-05-27T11:26:57Z 2014-05-27T11:26:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-64402012000500013 Brazilian Dental Journal, v. 23, n. 5, p. 547-554, 2012. 0103-6440 1806-4760 http://hdl.handle.net/11449/73540 10.1590/S0103-64402012000500013 S0103-64402012000500013 2-s2.0-84872400694 2-s2.0-84872400694.pdf 8000248781842587 5737127334248549 3003130522427820 0000-0002-7375-4714 |
url |
http://dx.doi.org/10.1590/S0103-64402012000500013 http://hdl.handle.net/11449/73540 |
identifier_str_mv |
Brazilian Dental Journal, v. 23, n. 5, p. 547-554, 2012. 0103-6440 1806-4760 10.1590/S0103-64402012000500013 S0103-64402012000500013 2-s2.0-84872400694 2-s2.0-84872400694.pdf 8000248781842587 5737127334248549 3003130522427820 0000-0002-7375-4714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
547-554 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789519239446528 |