Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2021.1954169 http://hdl.handle.net/11449/222112 |
Resumo: | Antimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 µM), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2)−1 (λ = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment. |
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Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilmsbiofilmchlorin e6hydrogen peroxideoxygenPhotodynamic therapyAntimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 µM), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2)−1 (λ = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment.Department of Periodontics Affiliated Stomatology Hospital of Guangzhou Medical University Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineState Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases Department of Periodontics West China Hospital of Stomatology Sichuan UniversityDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit AmsterdamDepartment of Chemistry Federal University of São Carlos–UFSCarSão Carlos Institute of Physics University of São Paulo-USP São CarlosDepartment of Restorative Dentistry School of Dentistry São Paulo State University-UNESPDepartment of Restorative Dentistry School of Dentistry São Paulo State University-UNESPGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineSichuan UniversityUniversity of Amsterdam and Vrije Universiteit AmsterdamUniversidade Federal de São Carlos (UFSCar)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Nie, MinSilva, Rodrigo Costa ede Oliveira, Kleber ThiagoBagnato, Vanderlei Salvadorde Souza Rastelli, Alessandra Nara [UNESP]Crielaard, WimYang, JingmeiDeng, Dong Mei2022-04-28T19:42:31Z2022-04-28T19:42:31Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article656-665http://dx.doi.org/10.1080/08927014.2021.1954169Biofouling, v. 37, n. 6, p. 656-665, 2021.1029-24540892-7014http://hdl.handle.net/11449/22211210.1080/08927014.2021.19541692-s2.0-85111710150Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofoulinginfo:eu-repo/semantics/openAccess2022-04-28T19:42:31Zoai:repositorio.unesp.br:11449/222112Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:42:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
title |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
spellingShingle |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms Nie, Min biofilm chlorin e6 hydrogen peroxide oxygen Photodynamic therapy |
title_short |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
title_full |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
title_fullStr |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
title_full_unstemmed |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
title_sort |
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms |
author |
Nie, Min |
author_facet |
Nie, Min Silva, Rodrigo Costa e de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador de Souza Rastelli, Alessandra Nara [UNESP] Crielaard, Wim Yang, Jingmei Deng, Dong Mei |
author_role |
author |
author2 |
Silva, Rodrigo Costa e de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador de Souza Rastelli, Alessandra Nara [UNESP] Crielaard, Wim Yang, Jingmei Deng, Dong Mei |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine Sichuan University University of Amsterdam and Vrije Universiteit Amsterdam Universidade Federal de São Carlos (UFSCar) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Nie, Min Silva, Rodrigo Costa e de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador de Souza Rastelli, Alessandra Nara [UNESP] Crielaard, Wim Yang, Jingmei Deng, Dong Mei |
dc.subject.por.fl_str_mv |
biofilm chlorin e6 hydrogen peroxide oxygen Photodynamic therapy |
topic |
biofilm chlorin e6 hydrogen peroxide oxygen Photodynamic therapy |
description |
Antimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 µM), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2)−1 (λ = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:42:31Z 2022-04-28T19:42:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2021.1954169 Biofouling, v. 37, n. 6, p. 656-665, 2021. 1029-2454 0892-7014 http://hdl.handle.net/11449/222112 10.1080/08927014.2021.1954169 2-s2.0-85111710150 |
url |
http://dx.doi.org/10.1080/08927014.2021.1954169 http://hdl.handle.net/11449/222112 |
identifier_str_mv |
Biofouling, v. 37, n. 6, p. 656-665, 2021. 1029-2454 0892-7014 10.1080/08927014.2021.1954169 2-s2.0-85111710150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
656-665 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965029807685632 |