Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia
Main Author: | |
---|---|
Publication Date: | 2021 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.intimp.2021.108366 http://hdl.handle.net/11449/229916 |
Summary: | Vitamin D (VD) is a multifunctional prohormone and low VD status in pregnancy may contribute to the risk of adverse perinatal outcomes, such as preeclampsia (PE). This molecule may modulate the polarization of T cell subsets during gestation. This study evaluated the in vitro immunomodulatory effect of VD [1,25(OH)2D3] on the gene expression of transcription factors and on cytokine production by T cell subsets. Twenty pregnant women with PE and twenty normotensive (NT) pregnant women were studied. Plasma concentration of VD, [25(OH)D3], was evaluated by chemiluminescence. PBMCs from preeclamptic and NT pregnant women were cultured in the absence or presence of VD to determine gene expression of T-bet (Th1), GATA-3 (Th2), RORγt, and RUNX1 (Th17), FoxP3 (regulatory T cell- Treg), and the receptors of VD (VDR) and IL-23 (IL-23R) by quantitative PCR. The concentration of cytokines in the PBMC supernatant culture was determined by cytometric bead array and ELISA immunoassay. The results showed that plasmatic levels of VD were significantly lower in the PE group. The treatment of PBMCs from PE pregnant women with VD induced downregulation of genes related to inflammatory profiles (Th1 and Th17), as well as an increase of the Th2 and Treg profiles. Thus, VD treatment decreased the release of IFN-γ, TNF-α, IL-17, IL-6, and IL-23 while it increased the levels of IL-10 in the PE group. VD induces an immunomodulatory effect in T cell subsets from pregnant women with PE, polarizing these cells to an anti-inflammatory and regulatory profile. |
id |
UNSP_78beeacec3b1137eb8b4aac6bf3b98d7 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229916 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsiaCytokineInflammationPBMCsPregnancyTh17TregVitamin D (VD) is a multifunctional prohormone and low VD status in pregnancy may contribute to the risk of adverse perinatal outcomes, such as preeclampsia (PE). This molecule may modulate the polarization of T cell subsets during gestation. This study evaluated the in vitro immunomodulatory effect of VD [1,25(OH)2D3] on the gene expression of transcription factors and on cytokine production by T cell subsets. Twenty pregnant women with PE and twenty normotensive (NT) pregnant women were studied. Plasma concentration of VD, [25(OH)D3], was evaluated by chemiluminescence. PBMCs from preeclamptic and NT pregnant women were cultured in the absence or presence of VD to determine gene expression of T-bet (Th1), GATA-3 (Th2), RORγt, and RUNX1 (Th17), FoxP3 (regulatory T cell- Treg), and the receptors of VD (VDR) and IL-23 (IL-23R) by quantitative PCR. The concentration of cytokines in the PBMC supernatant culture was determined by cytometric bead array and ELISA immunoassay. The results showed that plasmatic levels of VD were significantly lower in the PE group. The treatment of PBMCs from PE pregnant women with VD induced downregulation of genes related to inflammatory profiles (Th1 and Th17), as well as an increase of the Th2 and Treg profiles. Thus, VD treatment decreased the release of IFN-γ, TNF-α, IL-17, IL-6, and IL-23 while it increased the levels of IL-10 in the PE group. VD induces an immunomodulatory effect in T cell subsets from pregnant women with PE, polarizing these cells to an anti-inflammatory and regulatory profile.Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPUniversidade Estadual Paulista (UNESP)Ribeiro, Vanessa Rocha [UNESP]Romao-Veiga, Mariana [UNESP]Nunes, Priscila Rezeck [UNESP]Matias, Mariana Leticia [UNESP]Peracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2022-04-29T08:36:39Z2022-04-29T08:36:39Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.intimp.2021.108366International Immunopharmacology.1878-17051567-5769http://hdl.handle.net/11449/22991610.1016/j.intimp.2021.1083662-s2.0-85119448668Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Immunopharmacologyinfo:eu-repo/semantics/openAccess2022-04-29T08:36:39Zoai:repositorio.unesp.br:11449/229916Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:36:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
title |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
spellingShingle |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia Ribeiro, Vanessa Rocha [UNESP] Cytokine Inflammation PBMCs Pregnancy Th17 Treg |
title_short |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
title_full |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
title_fullStr |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
title_full_unstemmed |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
title_sort |
Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia |
author |
Ribeiro, Vanessa Rocha [UNESP] |
author_facet |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Matias, Mariana Leticia [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author_role |
author |
author2 |
Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Matias, Mariana Leticia [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Matias, Mariana Leticia [UNESP] Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
dc.subject.por.fl_str_mv |
Cytokine Inflammation PBMCs Pregnancy Th17 Treg |
topic |
Cytokine Inflammation PBMCs Pregnancy Th17 Treg |
description |
Vitamin D (VD) is a multifunctional prohormone and low VD status in pregnancy may contribute to the risk of adverse perinatal outcomes, such as preeclampsia (PE). This molecule may modulate the polarization of T cell subsets during gestation. This study evaluated the in vitro immunomodulatory effect of VD [1,25(OH)2D3] on the gene expression of transcription factors and on cytokine production by T cell subsets. Twenty pregnant women with PE and twenty normotensive (NT) pregnant women were studied. Plasma concentration of VD, [25(OH)D3], was evaluated by chemiluminescence. PBMCs from preeclamptic and NT pregnant women were cultured in the absence or presence of VD to determine gene expression of T-bet (Th1), GATA-3 (Th2), RORγt, and RUNX1 (Th17), FoxP3 (regulatory T cell- Treg), and the receptors of VD (VDR) and IL-23 (IL-23R) by quantitative PCR. The concentration of cytokines in the PBMC supernatant culture was determined by cytometric bead array and ELISA immunoassay. The results showed that plasmatic levels of VD were significantly lower in the PE group. The treatment of PBMCs from PE pregnant women with VD induced downregulation of genes related to inflammatory profiles (Th1 and Th17), as well as an increase of the Th2 and Treg profiles. Thus, VD treatment decreased the release of IFN-γ, TNF-α, IL-17, IL-6, and IL-23 while it increased the levels of IL-10 in the PE group. VD induces an immunomodulatory effect in T cell subsets from pregnant women with PE, polarizing these cells to an anti-inflammatory and regulatory profile. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:36:39Z 2022-04-29T08:36:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.intimp.2021.108366 International Immunopharmacology. 1878-1705 1567-5769 http://hdl.handle.net/11449/229916 10.1016/j.intimp.2021.108366 2-s2.0-85119448668 |
url |
http://dx.doi.org/10.1016/j.intimp.2021.108366 http://hdl.handle.net/11449/229916 |
identifier_str_mv |
International Immunopharmacology. 1878-1705 1567-5769 10.1016/j.intimp.2021.108366 2-s2.0-85119448668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Immunopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790098326028288 |