Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2016.06.003 http://hdl.handle.net/11449/173088 |
Resumo: | Objective This study aimed to evaluate the oxidative stress levels and the enzymatic and non-enzymatic antioxidant systems in saliva of toddlers with severe early childhood caries (S-ECC). Design Unstimulated saliva samples were collected at the morning from 0 to 3 year-old S-ECC (n = 30) or caries-free (CF) children (n = 30/group) for evaluation of oxidative stress (OS) and total antioxidant capacity (TAC), which were measured by the ferric reducing antioxidant power (FRAP) assay, as well as to assess the activity of enzymatic (superoxide dismutase, SOD) and non-enzymatic (uric acid, UA) antioxidant systems, respectively. Data were analyzed by Student's t-test (p < 0.05). Results Significantly higher protein levels were observed in saliva of S-ECC children (0.083 mg/mL) than in the CF group (0.070 mg/mL). Oxidative damage was significantly lower in saliva of S-ECC children (0.0019 μmol/L/mg protein) than in CF children (0.0039 μmol/L/mg protein), while salivary TAC (61.5 μmol/L), SOD activity (36.6 UE/mL) and uric acid (7.05 mg/mL) were significantly higher in saliva of S-ECC when compared to the CF group (49.1 μmol/L, 26.8 UE/mL and 5.02 mg/mL, respectively for TAC, SOD and UA). Conclusion Oxidative stress levels were significantly lower in saliva of S-ECC children, what might be associated with the increased activity of salivary enzymatic (SOD) and non-enzymatic (uric acid) antioxidant systems. |
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Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood cariesChildrenDental cariesOxidative stressSalivaSuperoxide dismutaseUric acidObjective This study aimed to evaluate the oxidative stress levels and the enzymatic and non-enzymatic antioxidant systems in saliva of toddlers with severe early childhood caries (S-ECC). Design Unstimulated saliva samples were collected at the morning from 0 to 3 year-old S-ECC (n = 30) or caries-free (CF) children (n = 30/group) for evaluation of oxidative stress (OS) and total antioxidant capacity (TAC), which were measured by the ferric reducing antioxidant power (FRAP) assay, as well as to assess the activity of enzymatic (superoxide dismutase, SOD) and non-enzymatic (uric acid, UA) antioxidant systems, respectively. Data were analyzed by Student's t-test (p < 0.05). Results Significantly higher protein levels were observed in saliva of S-ECC children (0.083 mg/mL) than in the CF group (0.070 mg/mL). Oxidative damage was significantly lower in saliva of S-ECC children (0.0019 μmol/L/mg protein) than in CF children (0.0039 μmol/L/mg protein), while salivary TAC (61.5 μmol/L), SOD activity (36.6 UE/mL) and uric acid (7.05 mg/mL) were significantly higher in saliva of S-ECC when compared to the CF group (49.1 μmol/L, 26.8 UE/mL and 5.02 mg/mL, respectively for TAC, SOD and UA). Conclusion Oxidative stress levels were significantly lower in saliva of S-ECC children, what might be associated with the increased activity of salivary enzymatic (SOD) and non-enzymatic (uric acid) antioxidant systems.Graduate Program in Dental Science Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio Araçatuba, 1193 Vila MendonçaMulticenter Graduate Program in Physiological Sciences Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaDepartment of Pediatric Dentistry and Public Heatlh Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaDepartment of Basic Sciences Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaGraduate Program in Dental Science Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio Araçatuba, 1193 Vila MendonçaMulticenter Graduate Program in Physiological Sciences Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaDepartment of Pediatric Dentistry and Public Heatlh Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaDepartment of Basic Sciences Araçatuba Dental School UNESP Univ. Estadual Paulista Rua José Bonifácio, 1193 Vila MendonçaUniversidade Estadual Paulista (Unesp)Silva, Priscila Vieira Da [UNESP]Troiano, Jéssica Antonini [UNESP]Nakamune, Ana Cláudia de Melo Stevanato [UNESP]Pessan, Juliano Pelim [UNESP]Antoniali, Cristina [UNESP]2018-12-11T17:03:35Z2018-12-11T17:03:35Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article62-66application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.06.003Archives of Oral Biology, v. 70, p. 62-66.1879-15060003-9969http://hdl.handle.net/11449/17308810.1016/j.archoralbio.2016.06.0032-s2.0-84975037758Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-01-15T06:19:26Zoai:repositorio.unesp.br:11449/173088Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-15T06:19:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
title |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
spellingShingle |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries Silva, Priscila Vieira Da [UNESP] Children Dental caries Oxidative stress Saliva Superoxide dismutase Uric acid |
title_short |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
title_full |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
title_fullStr |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
title_full_unstemmed |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
title_sort |
Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries |
author |
Silva, Priscila Vieira Da [UNESP] |
author_facet |
Silva, Priscila Vieira Da [UNESP] Troiano, Jéssica Antonini [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
author_role |
author |
author2 |
Troiano, Jéssica Antonini [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Priscila Vieira Da [UNESP] Troiano, Jéssica Antonini [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Children Dental caries Oxidative stress Saliva Superoxide dismutase Uric acid |
topic |
Children Dental caries Oxidative stress Saliva Superoxide dismutase Uric acid |
description |
Objective This study aimed to evaluate the oxidative stress levels and the enzymatic and non-enzymatic antioxidant systems in saliva of toddlers with severe early childhood caries (S-ECC). Design Unstimulated saliva samples were collected at the morning from 0 to 3 year-old S-ECC (n = 30) or caries-free (CF) children (n = 30/group) for evaluation of oxidative stress (OS) and total antioxidant capacity (TAC), which were measured by the ferric reducing antioxidant power (FRAP) assay, as well as to assess the activity of enzymatic (superoxide dismutase, SOD) and non-enzymatic (uric acid, UA) antioxidant systems, respectively. Data were analyzed by Student's t-test (p < 0.05). Results Significantly higher protein levels were observed in saliva of S-ECC children (0.083 mg/mL) than in the CF group (0.070 mg/mL). Oxidative damage was significantly lower in saliva of S-ECC children (0.0019 μmol/L/mg protein) than in CF children (0.0039 μmol/L/mg protein), while salivary TAC (61.5 μmol/L), SOD activity (36.6 UE/mL) and uric acid (7.05 mg/mL) were significantly higher in saliva of S-ECC when compared to the CF group (49.1 μmol/L, 26.8 UE/mL and 5.02 mg/mL, respectively for TAC, SOD and UA). Conclusion Oxidative stress levels were significantly lower in saliva of S-ECC children, what might be associated with the increased activity of salivary enzymatic (SOD) and non-enzymatic (uric acid) antioxidant systems. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-12-11T17:03:35Z 2018-12-11T17:03:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2016.06.003 Archives of Oral Biology, v. 70, p. 62-66. 1879-1506 0003-9969 http://hdl.handle.net/11449/173088 10.1016/j.archoralbio.2016.06.003 2-s2.0-84975037758 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2016.06.003 http://hdl.handle.net/11449/173088 |
identifier_str_mv |
Archives of Oral Biology, v. 70, p. 62-66. 1879-1506 0003-9969 10.1016/j.archoralbio.2016.06.003 2-s2.0-84975037758 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
62-66 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965621867249664 |