Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle
Main Author: | |
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Publication Date: | 2013 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1371/journal.pone.0081397 http://hdl.handle.net/11449/111537 |
Summary: | Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus. |
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Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell CycleHere, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista UNESP, Inst Biociencias, Dept Genet, Sao Paulo, BrazilUniv Estadual Campinas UNICAMP, Sao Paulo, BrazilEmpresa Brasileira Pesquisa Agr Embrapa Caprinos, Sobral, Ceara, BrazilSeattle Biomed Res Inst, Seattle, WA 98109 USACtr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias LNBio, Sao Paulo, BrazilInst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo, BrazilUniv Estadual Paulista UNESP, Inst Biociencias, Dept Genet, Sao Paulo, BrazilFAPESP: 12/50263-5CNPq: 4744252010-0CNPq: PDE 202223/2012-4Public Library ScienceUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Seattle Biomed Res InstCtr Nacl Pesquisa Energia & MatInstituto ButantanSilva, Marcelo Santos da [UNESP]Monteiro, Jomar PatricioNunes, Vinicius Santana [UNESP]Vasconcelos, Elton JosePerez, Arina Marina [UNESP]Freitas-Junior, Lucio de HolandaElias, Maria CarolinaNogueira Cano, Maria Isabel [UNESP]2014-12-03T13:08:44Z2014-12-03T13:08:44Z2013-11-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.1371/journal.pone.0081397Plos One. San Francisco: Public Library Science, v. 8, n. 11, 12 p., 2013.1932-6203http://hdl.handle.net/11449/11153710.1371/journal.pone.0081397WOS:000327539800128WOS000327539800128.pdf7449821021440644Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-10-28T06:10:22Zoai:repositorio.unesp.br:11449/111537Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-28T06:10:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
title |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
spellingShingle |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle Silva, Marcelo Santos da [UNESP] |
title_short |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
title_full |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
title_fullStr |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
title_full_unstemmed |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
title_sort |
Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle |
author |
Silva, Marcelo Santos da [UNESP] |
author_facet |
Silva, Marcelo Santos da [UNESP] Monteiro, Jomar Patricio Nunes, Vinicius Santana [UNESP] Vasconcelos, Elton Jose Perez, Arina Marina [UNESP] Freitas-Junior, Lucio de Holanda Elias, Maria Carolina Nogueira Cano, Maria Isabel [UNESP] |
author_role |
author |
author2 |
Monteiro, Jomar Patricio Nunes, Vinicius Santana [UNESP] Vasconcelos, Elton Jose Perez, Arina Marina [UNESP] Freitas-Junior, Lucio de Holanda Elias, Maria Carolina Nogueira Cano, Maria Isabel [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Seattle Biomed Res Inst Ctr Nacl Pesquisa Energia & Mat Instituto Butantan |
dc.contributor.author.fl_str_mv |
Silva, Marcelo Santos da [UNESP] Monteiro, Jomar Patricio Nunes, Vinicius Santana [UNESP] Vasconcelos, Elton Jose Perez, Arina Marina [UNESP] Freitas-Junior, Lucio de Holanda Elias, Maria Carolina Nogueira Cano, Maria Isabel [UNESP] |
description |
Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-21 2014-12-03T13:08:44Z 2014-12-03T13:08:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0081397 Plos One. San Francisco: Public Library Science, v. 8, n. 11, 12 p., 2013. 1932-6203 http://hdl.handle.net/11449/111537 10.1371/journal.pone.0081397 WOS:000327539800128 WOS000327539800128.pdf 7449821021440644 |
url |
http://dx.doi.org/10.1371/journal.pone.0081397 http://hdl.handle.net/11449/111537 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 11, 12 p., 2013. 1932-6203 10.1371/journal.pone.0081397 WOS:000327539800128 WOS000327539800128.pdf 7449821021440644 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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