ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2021.104918 http://hdl.handle.net/11449/207735 |
Resumo: | Tambaqui Colossoma macropomum is the most cultivated native fish in South America and Aeromonas hydrophila is one of the main bacteria infecting tropical fish. Despite the economic importance of this round fish, to date, there has been a paucity of investigations into haematological changes in tambaqui. In this study, detailed blood analyses (0 h, 6 h, 24 h, 7 d and 14 d) following intraperitoneal challenge with A. hydrophila were performed. After analysing the results, there was a suspicion of a novel cell death mechanism via extracellular traps (ETosis) in tambaqui. The search for ETosis was based on differential interference contrast (DIC) microscopy and scanning electron microscopy (SEM) assays through application of an adapted protocol applying co-incubation of leukocytes with A. hydrophila. The cells were investigated at: 0 h (control), 4 h and 7 h after incubation. The complete haemogram profile showed an uncommon severe leukopenia in early phases of infection (6 h, p < 0.001 and ≤ 0.05), due to significant decreases in the three main leukocytes: lymphocytes (6 h, p ≤ 0.001), monocytes (6 h, p ≤ 0.05) and neutrophils (6 h and 24 h, p ≤ 0.01 and p ≤ 0.05). Leucocytosis and lymphocytosis (p ≤ 0.01) were ascertained only 7 days post-infection. Through DIC and SEM, we discovered that leukocyte suicide exposed the nuclear contents between 4 and 7 h after stimuli with bacteria. The leukogram profile associated with DIC and SEM analyses suggested that tambaqui leukocytes underwent a programmed death (ETosis) in order to expose chromatin and granule proteins as a trap to bind and then kill bacteria; thus, preventing A. hydrophila from spreading and resulting in leukopenia during the early phase of bacterial infection. In this paper, we presume that ETosis is one of the last resources for tambaqui to contain the infection, and after this leukocyte strategy, a high number of phagocytic cells are produced and released into the peripheral circulation. |
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ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune responseAeromonasAquacultureExtracellular trapHemorrhagic septicemiaNative fishNETosisTambaqui Colossoma macropomum is the most cultivated native fish in South America and Aeromonas hydrophila is one of the main bacteria infecting tropical fish. Despite the economic importance of this round fish, to date, there has been a paucity of investigations into haematological changes in tambaqui. In this study, detailed blood analyses (0 h, 6 h, 24 h, 7 d and 14 d) following intraperitoneal challenge with A. hydrophila were performed. After analysing the results, there was a suspicion of a novel cell death mechanism via extracellular traps (ETosis) in tambaqui. The search for ETosis was based on differential interference contrast (DIC) microscopy and scanning electron microscopy (SEM) assays through application of an adapted protocol applying co-incubation of leukocytes with A. hydrophila. The cells were investigated at: 0 h (control), 4 h and 7 h after incubation. The complete haemogram profile showed an uncommon severe leukopenia in early phases of infection (6 h, p < 0.001 and ≤ 0.05), due to significant decreases in the three main leukocytes: lymphocytes (6 h, p ≤ 0.001), monocytes (6 h, p ≤ 0.05) and neutrophils (6 h and 24 h, p ≤ 0.01 and p ≤ 0.05). Leucocytosis and lymphocytosis (p ≤ 0.01) were ascertained only 7 days post-infection. Through DIC and SEM, we discovered that leukocyte suicide exposed the nuclear contents between 4 and 7 h after stimuli with bacteria. The leukogram profile associated with DIC and SEM analyses suggested that tambaqui leukocytes underwent a programmed death (ETosis) in order to expose chromatin and granule proteins as a trap to bind and then kill bacteria; thus, preventing A. hydrophila from spreading and resulting in leukopenia during the early phase of bacterial infection. In this paper, we presume that ETosis is one of the last resources for tambaqui to contain the infection, and after this leukocyte strategy, a high number of phagocytic cells are produced and released into the peripheral circulation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Nilton Lins University (UNL) Laboratory of Microbiology Applied to Aquatic OrganismsNilton Lins University (UNL) Laboratory of Parasitology and Pathology of Aquatic OrganismsSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic Organisms, São PauloSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and Conservation, São PauloWageningen University & Research (WUR) Aquaculture and Fisheries GroupSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic Organisms, São PauloSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and Conservation, São PauloCNPq: 140431/2016-0CNPq: 305007/2016-5CNPq: 431713/2016-2Laboratory of Microbiology Applied to Aquatic OrganismsLaboratory of Parasitology and Pathology of Aquatic OrganismsUniversidade Estadual Paulista (Unesp)Aquaculture and Fisheries GroupGallani, Sílvia UmedaValladão, Gustavo Moraes RamosAlves, Lindomar de Oliveira [UNESP]Jesus, Raphael Barbetta de [UNESP]Kotzent, Suzana [UNESP]Hashimoto, Diogo Teruo [UNESP]Wiegertjes, GeertPilarski, Fabiana [UNESP]2021-06-25T11:00:03Z2021-06-25T11:00:03Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.micpath.2021.104918Microbial Pathogenesis, v. 155.1096-12080882-4010http://hdl.handle.net/11449/20773510.1016/j.micpath.2021.1049182-s2.0-85105837034Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesisinfo:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/207735Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
title |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
spellingShingle |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response Gallani, Sílvia Umeda Aeromonas Aquaculture Extracellular trap Hemorrhagic septicemia Native fish NETosis |
title_short |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
title_full |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
title_fullStr |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
title_full_unstemmed |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
title_sort |
ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response |
author |
Gallani, Sílvia Umeda |
author_facet |
Gallani, Sílvia Umeda Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Jesus, Raphael Barbetta de [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Pilarski, Fabiana [UNESP] |
author_role |
author |
author2 |
Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Jesus, Raphael Barbetta de [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Pilarski, Fabiana [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Microbiology Applied to Aquatic Organisms Laboratory of Parasitology and Pathology of Aquatic Organisms Universidade Estadual Paulista (Unesp) Aquaculture and Fisheries Group |
dc.contributor.author.fl_str_mv |
Gallani, Sílvia Umeda Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Jesus, Raphael Barbetta de [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Pilarski, Fabiana [UNESP] |
dc.subject.por.fl_str_mv |
Aeromonas Aquaculture Extracellular trap Hemorrhagic septicemia Native fish NETosis |
topic |
Aeromonas Aquaculture Extracellular trap Hemorrhagic septicemia Native fish NETosis |
description |
Tambaqui Colossoma macropomum is the most cultivated native fish in South America and Aeromonas hydrophila is one of the main bacteria infecting tropical fish. Despite the economic importance of this round fish, to date, there has been a paucity of investigations into haematological changes in tambaqui. In this study, detailed blood analyses (0 h, 6 h, 24 h, 7 d and 14 d) following intraperitoneal challenge with A. hydrophila were performed. After analysing the results, there was a suspicion of a novel cell death mechanism via extracellular traps (ETosis) in tambaqui. The search for ETosis was based on differential interference contrast (DIC) microscopy and scanning electron microscopy (SEM) assays through application of an adapted protocol applying co-incubation of leukocytes with A. hydrophila. The cells were investigated at: 0 h (control), 4 h and 7 h after incubation. The complete haemogram profile showed an uncommon severe leukopenia in early phases of infection (6 h, p < 0.001 and ≤ 0.05), due to significant decreases in the three main leukocytes: lymphocytes (6 h, p ≤ 0.001), monocytes (6 h, p ≤ 0.05) and neutrophils (6 h and 24 h, p ≤ 0.01 and p ≤ 0.05). Leucocytosis and lymphocytosis (p ≤ 0.01) were ascertained only 7 days post-infection. Through DIC and SEM, we discovered that leukocyte suicide exposed the nuclear contents between 4 and 7 h after stimuli with bacteria. The leukogram profile associated with DIC and SEM analyses suggested that tambaqui leukocytes underwent a programmed death (ETosis) in order to expose chromatin and granule proteins as a trap to bind and then kill bacteria; thus, preventing A. hydrophila from spreading and resulting in leukopenia during the early phase of bacterial infection. In this paper, we presume that ETosis is one of the last resources for tambaqui to contain the infection, and after this leukocyte strategy, a high number of phagocytic cells are produced and released into the peripheral circulation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:00:03Z 2021-06-25T11:00:03Z 2021-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2021.104918 Microbial Pathogenesis, v. 155. 1096-1208 0882-4010 http://hdl.handle.net/11449/207735 10.1016/j.micpath.2021.104918 2-s2.0-85105837034 |
url |
http://dx.doi.org/10.1016/j.micpath.2021.104918 http://hdl.handle.net/11449/207735 |
identifier_str_mv |
Microbial Pathogenesis, v. 155. 1096-1208 0882-4010 10.1016/j.micpath.2021.104918 2-s2.0-85105837034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790160410116096 |