Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/antibiotics12020329 http://hdl.handle.net/11449/248420 |
Resumo: | The effects of extracts, fractions, and molecules of Casearia sylvestris to control the cariogenic biofilm of Streptococcus mutans were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged S. mutans cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from C. sylvestris. |
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Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic BiofilmbiofilmCasearia sylvestrisdental cariesextracellular matrixStreptococcus mutansThe effects of extracts, fractions, and molecules of Casearia sylvestris to control the cariogenic biofilm of Streptococcus mutans were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged S. mutans cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from C. sylvestris.Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SPDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), SPDepartment of Physics and Chemistry Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP), SPDepartment of Organic Chemistry Institute of Chemistry Federal University of Alfenas, MGDepartment of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP), SPDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SPDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Federal University of AlfenasUniversidade Estadual de Campinas (UNICAMP)Ribeiro, Sabrina M. [UNESP]Bueno, Paula C. P. [UNESP]Cavalheiro, Alberto José [UNESP]Klein, Marlise I. [UNESP]2023-07-29T13:43:36Z2023-07-29T13:43:36Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/antibiotics12020329Antibiotics, v. 12, n. 2, 2023.2079-6382http://hdl.handle.net/11449/24842010.3390/antibiotics120203292-s2.0-85148892072Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntibioticsinfo:eu-repo/semantics/openAccess2023-07-29T13:43:36Zoai:repositorio.unesp.br:11449/248420Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:43:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
title |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
spellingShingle |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm Ribeiro, Sabrina M. [UNESP] biofilm Casearia sylvestris dental caries extracellular matrix Streptococcus mutans |
title_short |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
title_full |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
title_fullStr |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
title_full_unstemmed |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
title_sort |
Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm |
author |
Ribeiro, Sabrina M. [UNESP] |
author_facet |
Ribeiro, Sabrina M. [UNESP] Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
author_role |
author |
author2 |
Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Federal University of Alfenas Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Ribeiro, Sabrina M. [UNESP] Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
dc.subject.por.fl_str_mv |
biofilm Casearia sylvestris dental caries extracellular matrix Streptococcus mutans |
topic |
biofilm Casearia sylvestris dental caries extracellular matrix Streptococcus mutans |
description |
The effects of extracts, fractions, and molecules of Casearia sylvestris to control the cariogenic biofilm of Streptococcus mutans were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged S. mutans cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from C. sylvestris. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:43:36Z 2023-07-29T13:43:36Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/antibiotics12020329 Antibiotics, v. 12, n. 2, 2023. 2079-6382 http://hdl.handle.net/11449/248420 10.3390/antibiotics12020329 2-s2.0-85148892072 |
url |
http://dx.doi.org/10.3390/antibiotics12020329 http://hdl.handle.net/11449/248420 |
identifier_str_mv |
Antibiotics, v. 12, n. 2, 2023. 2079-6382 10.3390/antibiotics12020329 2-s2.0-85148892072 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antibiotics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790258922782720 |