Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://journal.frontiersin.org/article/10.3389/fcimb.2015.00010/full http://hdl.handle.net/11449/128535 |
Resumo: | Biofilms are highly structured microbial communities that are enmeshed in a self-produced extracellular matrix. Within the complex oral microbiome, Streptococcus mutans is a major producer of extracellular polymeric substances including exopolysaccharides (EPS), eDNA, and lipoteichoic acid (LTA). EPS produced by S. mutans-derived exoenzymes promote local accumulation of microbes on the teeth, while forming a spatially heterogeneous and diffusion-limiting matrix that protects embedded bacteria. The EPS-rich matrix provides mechanical stability/cohesiveness and facilitates the creation of highly acidic microenvironments, which are critical for the pathogenesis of dental caries. In parallel, S. mutans also releases eDNA and LTA, which can contribute with matrix development. eDNA enhances EPS (glucan) synthesis locally, increasing the adhesion of S. mutans to saliva-coated apatitic surfaces and the assembly of highly cohesive biofilms. eDNA and other extracellular substances, acting in concert with EPS, may impact the functional properties of the matrix and the virulence of cariogenic biofilms. Enhanced understanding about the assembly principles of the matrix may lead to efficacious approaches to control biofilm-related diseases. |
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Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilmsBiofilmsDental cariesStreptococcus mutansExtracellular matrixSpatial heterogeneitiesMechanical stabilityExopolysaccharideseDNABiofilms are highly structured microbial communities that are enmeshed in a self-produced extracellular matrix. Within the complex oral microbiome, Streptococcus mutans is a major producer of extracellular polymeric substances including exopolysaccharides (EPS), eDNA, and lipoteichoic acid (LTA). EPS produced by S. mutans-derived exoenzymes promote local accumulation of microbes on the teeth, while forming a spatially heterogeneous and diffusion-limiting matrix that protects embedded bacteria. The EPS-rich matrix provides mechanical stability/cohesiveness and facilitates the creation of highly acidic microenvironments, which are critical for the pathogenesis of dental caries. In parallel, S. mutans also releases eDNA and LTA, which can contribute with matrix development. eDNA enhances EPS (glucan) synthesis locally, increasing the adhesion of S. mutans to saliva-coated apatitic surfaces and the assembly of highly cohesive biofilms. eDNA and other extracellular substances, acting in concert with EPS, may impact the functional properties of the matrix and the virulence of cariogenic biofilms. Enhanced understanding about the assembly principles of the matrix may lead to efficacious approaches to control biofilm-related diseases.National Science FoundationNational Institute for Dental and Craniofacial ResearchFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center for Oral Biology, University of Rochester, Rochester, NY, USABiofilm Research Lab, Levy Center for Oral Health, Department of Orthodontics and Divisions of Pediatric Dentistry and Community Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USAWhistler Center for Carbohydrate Research, Purdue University, West Lafayette, IN, USADepartment of Dental Materials and Prosthodontics, Araraquara Dental School, Universidade Estadual Paulista “Júlio de Mesquita Filho” , UNESP, Araraquara, BrazilNational Science Foundation: EFRI-1137186National Institute for Dental and Craniofacial Research: DE16139/DE18023National Institute for Dental and Craniofacial Research: R90DE022529FAPESP: 2014/05423-0Frontiers Research FoundationUniversity of RochesterUniversity of PennsylvaniaPurdue UniversityUniversidade Estadual Paulista (Unesp)Klein, Marlise I.Hwang, GeelsuSantos, Paulo H. S.Campanella, Osvaldo H.Koo, Hyun2015-10-21T13:10:45Z2015-10-21T13:10:45Z2015-02-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://journal.frontiersin.org/article/10.3389/fcimb.2015.00010/fullFrontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Research Foundation, v. 5, p. 1-8, 2015.2235-2988http://hdl.handle.net/11449/12853510.3389/fcimb.2015.00010WOS:000349282700001WOS000349282700001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Cellular And Infection Microbiology3.5201,703info:eu-repo/semantics/openAccess2024-01-04T06:29:38Zoai:repositorio.unesp.br:11449/128535Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-04T06:29:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
title |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
spellingShingle |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms Klein, Marlise I. Biofilms Dental caries Streptococcus mutans Extracellular matrix Spatial heterogeneities Mechanical stability Exopolysaccharides eDNA |
title_short |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
title_full |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
title_fullStr |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
title_full_unstemmed |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
title_sort |
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms |
author |
Klein, Marlise I. |
author_facet |
Klein, Marlise I. Hwang, Geelsu Santos, Paulo H. S. Campanella, Osvaldo H. Koo, Hyun |
author_role |
author |
author2 |
Hwang, Geelsu Santos, Paulo H. S. Campanella, Osvaldo H. Koo, Hyun |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of Rochester University of Pennsylvania Purdue University Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Klein, Marlise I. Hwang, Geelsu Santos, Paulo H. S. Campanella, Osvaldo H. Koo, Hyun |
dc.subject.por.fl_str_mv |
Biofilms Dental caries Streptococcus mutans Extracellular matrix Spatial heterogeneities Mechanical stability Exopolysaccharides eDNA |
topic |
Biofilms Dental caries Streptococcus mutans Extracellular matrix Spatial heterogeneities Mechanical stability Exopolysaccharides eDNA |
description |
Biofilms are highly structured microbial communities that are enmeshed in a self-produced extracellular matrix. Within the complex oral microbiome, Streptococcus mutans is a major producer of extracellular polymeric substances including exopolysaccharides (EPS), eDNA, and lipoteichoic acid (LTA). EPS produced by S. mutans-derived exoenzymes promote local accumulation of microbes on the teeth, while forming a spatially heterogeneous and diffusion-limiting matrix that protects embedded bacteria. The EPS-rich matrix provides mechanical stability/cohesiveness and facilitates the creation of highly acidic microenvironments, which are critical for the pathogenesis of dental caries. In parallel, S. mutans also releases eDNA and LTA, which can contribute with matrix development. eDNA enhances EPS (glucan) synthesis locally, increasing the adhesion of S. mutans to saliva-coated apatitic surfaces and the assembly of highly cohesive biofilms. eDNA and other extracellular substances, acting in concert with EPS, may impact the functional properties of the matrix and the virulence of cariogenic biofilms. Enhanced understanding about the assembly principles of the matrix may lead to efficacious approaches to control biofilm-related diseases. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:10:45Z 2015-10-21T13:10:45Z 2015-02-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://journal.frontiersin.org/article/10.3389/fcimb.2015.00010/full Frontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Research Foundation, v. 5, p. 1-8, 2015. 2235-2988 http://hdl.handle.net/11449/128535 10.3389/fcimb.2015.00010 WOS:000349282700001 WOS000349282700001.pdf |
url |
http://journal.frontiersin.org/article/10.3389/fcimb.2015.00010/full http://hdl.handle.net/11449/128535 |
identifier_str_mv |
Frontiers In Cellular And Infection Microbiology. Lausanne: Frontiers Research Foundation, v. 5, p. 1-8, 2015. 2235-2988 10.3389/fcimb.2015.00010 WOS:000349282700001 WOS000349282700001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Cellular And Infection Microbiology 3.520 1,703 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Research Foundation |
publisher.none.fl_str_mv |
Frontiers Research Foundation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965517858996224 |