Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions
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Publication Date: | 2013 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/S0104-66322013000200004 http://hdl.handle.net/11449/25247 |
Summary: | The effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L-1. Highest CephC production (566.5 mg.L-1) was obtained in fed-batch cultivation with glycerol feeding. In fed-batch experiments performed with starch feeding, the production of CephC was in general higher than that of CA. A dissociation index (DI) was used to identify feeding conditions that favored production of CephC relative to CA. In all cultures with glycerol, DI values were less than unity, indicating higher production of CA compared to CephC. Conversely, in cultures fed with starch, the DI values obtained were greater than unity. However, no carbon source or feeding condition was able to completely dissociate the production of CA from that of CephC. |
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Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditionsStreptomyces clavuligerusClavulanic acidCephamycin CFed-batchThe effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L-1. Highest CephC production (566.5 mg.L-1) was obtained in fed-batch cultivation with glycerol feeding. In fed-batch experiments performed with starch feeding, the production of CephC was in general higher than that of CA. A dissociation index (DI) was used to identify feeding conditions that favored production of CephC relative to CA. In all cultures with glycerol, DI values were less than unity, indicating higher production of CA compared to CephC. Conversely, in cultures fed with starch, the DI values obtained were greater than unity. However, no carbon source or feeding condition was able to completely dissociate the production of CA from that of CephC.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Carlos (UFSCar) Department of Chemical EngineeringUniversidade Estadual Paulista Institute of Chemistry Department of Biochemistry and TechnologyUniversidade Estadual Paulista Institute of Chemistry Department of Biochemistry and TechnologyFAPESP: 05/55079-4FAPESP: 11/23807-1CNPq: 474295/2009-5Brazilian Society of Chemical EngineeringUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Bellão, C.Antonio, T. [UNESP]Araujo, Maria Lucia Gonsales da Costa [UNESP]Badino, A. C.2014-05-20T14:17:31Z2014-05-20T14:17:31Z2013-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article257-266application/pdfhttp://dx.doi.org/10.1590/S0104-66322013000200004Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 30, n. 2, p. 257-266, 2013.0104-6632http://hdl.handle.net/11449/2524710.1590/S0104-66322013000200004S0104-66322013000200004WOS:000319989500004S0104-66322013000200004.pdf2478051365107560SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Chemical Engineering0.9250,395info:eu-repo/semantics/openAccess2023-12-24T06:19:29Zoai:repositorio.unesp.br:11449/25247Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:19:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
title |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
spellingShingle |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions Bellão, C. Streptomyces clavuligerus Clavulanic acid Cephamycin C Fed-batch |
title_short |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
title_full |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
title_fullStr |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
title_full_unstemmed |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
title_sort |
Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions |
author |
Bellão, C. |
author_facet |
Bellão, C. Antonio, T. [UNESP] Araujo, Maria Lucia Gonsales da Costa [UNESP] Badino, A. C. |
author_role |
author |
author2 |
Antonio, T. [UNESP] Araujo, Maria Lucia Gonsales da Costa [UNESP] Badino, A. C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bellão, C. Antonio, T. [UNESP] Araujo, Maria Lucia Gonsales da Costa [UNESP] Badino, A. C. |
dc.subject.por.fl_str_mv |
Streptomyces clavuligerus Clavulanic acid Cephamycin C Fed-batch |
topic |
Streptomyces clavuligerus Clavulanic acid Cephamycin C Fed-batch |
description |
The effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L-1. Highest CephC production (566.5 mg.L-1) was obtained in fed-batch cultivation with glycerol feeding. In fed-batch experiments performed with starch feeding, the production of CephC was in general higher than that of CA. A dissociation index (DI) was used to identify feeding conditions that favored production of CephC relative to CA. In all cultures with glycerol, DI values were less than unity, indicating higher production of CA compared to CephC. Conversely, in cultures fed with starch, the DI values obtained were greater than unity. However, no carbon source or feeding condition was able to completely dissociate the production of CA from that of CephC. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 2014-05-20T14:17:31Z 2014-05-20T14:17:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0104-66322013000200004 Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 30, n. 2, p. 257-266, 2013. 0104-6632 http://hdl.handle.net/11449/25247 10.1590/S0104-66322013000200004 S0104-66322013000200004 WOS:000319989500004 S0104-66322013000200004.pdf 2478051365107560 |
url |
http://dx.doi.org/10.1590/S0104-66322013000200004 http://hdl.handle.net/11449/25247 |
identifier_str_mv |
Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 30, n. 2, p. 257-266, 2013. 0104-6632 10.1590/S0104-66322013000200004 S0104-66322013000200004 WOS:000319989500004 S0104-66322013000200004.pdf 2478051365107560 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Chemical Engineering 0.925 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
257-266 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790097491361792 |