INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0104-66322010000400001 http://hdl.handle.net/11449/25287 |
Resumo: | The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp(max)) ranged randomly from 430 to 560 mg.L(-1), with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp(max)) of 13.7 mg.L(-1). h(-1) was obtained with 0.66 g.L(-1) of ornithine, 44.2% higher than the Pp(max) in the control run. In fed-batch experiments, Cp(max) varied within the narrow range from 1.254 to 1.405 g.L(-1), 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp(max), although it influenced only slightly the Cp(max). Concerning glycerol, the highest CA production of 1.6 g.L(-1) was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L(-1)) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6: 1), on CA biosynthesis. |
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INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerusClavulanic acidStreptomyces clavuligerusGlycerolOrnithineFed-batchThe influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp(max)) ranged randomly from 430 to 560 mg.L(-1), with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp(max)) of 13.7 mg.L(-1). h(-1) was obtained with 0.66 g.L(-1) of ornithine, 44.2% higher than the Pp(max) in the control run. In fed-batch experiments, Cp(max) varied within the narrow range from 1.254 to 1.405 g.L(-1), 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp(max), although it influenced only slightly the Cp(max). Concerning glycerol, the highest CA production of 1.6 g.L(-1) was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L(-1)) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6: 1), on CA biosynthesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Carlos (UFSCar), Dept Chem Engn, BR-13565905 São Carlos, SP, BrazilSão Paulo State Univ, UNESP, Inst Chem, Dept Biochem & Technol Chem, BR-14800900 Araraquara, SP, BrazilSão Paulo State Univ, UNESP, Inst Chem, Dept Biochem & Technol Chem, BR-14800900 Araraquara, SP, BrazilFAPESP: 03/11722-5FAPESP: 05/55079-4CNPq: 474295/2009-5Brazilian Soc Chemical EngUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Teodoro, J. C.Baptista-Neto, A.Araujo, Maria Lucia Gonsales da Costa [UNESP]Hokka, C. O.Badino, A. C.2014-05-20T14:17:38Z2014-05-20T14:17:38Z2010-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article499-506application/pdfhttp://dx.doi.org/10.1590/S0104-66322010000400001Brazilian Journal of Chemical Engineering. São Paulo: Brazilian Soc Chemical Eng, v. 27, n. 4, p. 499-506, 2010.0104-6632http://hdl.handle.net/11449/25287S0104-66322010000400001WOS:000286106200001S0104-66322010000400001.pdf2478051365107560Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Chemical Engineering0.9250,395info:eu-repo/semantics/openAccess2024-01-19T06:26:36Zoai:repositorio.unesp.br:11449/25287Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-19T06:26:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
title |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
spellingShingle |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus Teodoro, J. C. Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
title_short |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
title_full |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
title_fullStr |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
title_full_unstemmed |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
title_sort |
INFLUENCE of GLYCEROL and ORNITHINE FEEDING on CLAVULANIC ACID PRODUCTION BY Streptomyces clavuligerus |
author |
Teodoro, J. C. |
author_facet |
Teodoro, J. C. Baptista-Neto, A. Araujo, Maria Lucia Gonsales da Costa [UNESP] Hokka, C. O. Badino, A. C. |
author_role |
author |
author2 |
Baptista-Neto, A. Araujo, Maria Lucia Gonsales da Costa [UNESP] Hokka, C. O. Badino, A. C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Teodoro, J. C. Baptista-Neto, A. Araujo, Maria Lucia Gonsales da Costa [UNESP] Hokka, C. O. Badino, A. C. |
dc.subject.por.fl_str_mv |
Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
topic |
Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
description |
The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp(max)) ranged randomly from 430 to 560 mg.L(-1), with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp(max)) of 13.7 mg.L(-1). h(-1) was obtained with 0.66 g.L(-1) of ornithine, 44.2% higher than the Pp(max) in the control run. In fed-batch experiments, Cp(max) varied within the narrow range from 1.254 to 1.405 g.L(-1), 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp(max), although it influenced only slightly the Cp(max). Concerning glycerol, the highest CA production of 1.6 g.L(-1) was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L(-1)) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6: 1), on CA biosynthesis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 2014-05-20T14:17:38Z 2014-05-20T14:17:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0104-66322010000400001 Brazilian Journal of Chemical Engineering. São Paulo: Brazilian Soc Chemical Eng, v. 27, n. 4, p. 499-506, 2010. 0104-6632 http://hdl.handle.net/11449/25287 S0104-66322010000400001 WOS:000286106200001 S0104-66322010000400001.pdf 2478051365107560 |
url |
http://dx.doi.org/10.1590/S0104-66322010000400001 http://hdl.handle.net/11449/25287 |
identifier_str_mv |
Brazilian Journal of Chemical Engineering. São Paulo: Brazilian Soc Chemical Eng, v. 27, n. 4, p. 499-506, 2010. 0104-6632 S0104-66322010000400001 WOS:000286106200001 S0104-66322010000400001.pdf 2478051365107560 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Chemical Engineering 0.925 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
499-506 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Soc Chemical Eng |
publisher.none.fl_str_mv |
Brazilian Soc Chemical Eng |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965661297901568 |