Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules25092232 http://hdl.handle.net/11449/198825 |
Resumo: | Dental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 μg/mL), myricetin (500 μg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. |
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Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulationBiofilmCompound 1771ExopolysaccharidesLipoteichoic acidsMyricetinStreptococcusmutansDental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 μg/mL), myricetin (500 μg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), Rua Humaita, 1680Faculty of Health Sciences University of MagdalenaDepartment of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), Rua Humaita, 1680FAPESP: #2014/05423-0Universidade Estadual Paulista (Unesp)University of MagdalenaPedraza, Midian C. Castillo [UNESP]De Oliveira Fratucelli, Erick Dante [UNESP]Ribeiro, Sabrina Marcela [UNESP]Salamanca, Elkin Jahir Florez [UNESP]Da Silva Colin, Jaqueline [UNESP]Klein, Marlise I. [UNESP]2020-12-12T01:23:01Z2020-12-12T01:23:01Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules25092232Molecules, v. 25, n. 9, 2020.1420-3049http://hdl.handle.net/11449/19882510.3390/molecules250922322-s2.0-85084501969Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2021-10-22T20:36:18Zoai:repositorio.unesp.br:11449/198825Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:36:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
title |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
spellingShingle |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation Pedraza, Midian C. Castillo [UNESP] Biofilm Compound 1771 Exopolysaccharides Lipoteichoic acids Myricetin Streptococcusmutans |
title_short |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
title_full |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
title_fullStr |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
title_full_unstemmed |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
title_sort |
Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation |
author |
Pedraza, Midian C. Castillo [UNESP] |
author_facet |
Pedraza, Midian C. Castillo [UNESP] De Oliveira Fratucelli, Erick Dante [UNESP] Ribeiro, Sabrina Marcela [UNESP] Salamanca, Elkin Jahir Florez [UNESP] Da Silva Colin, Jaqueline [UNESP] Klein, Marlise I. [UNESP] |
author_role |
author |
author2 |
De Oliveira Fratucelli, Erick Dante [UNESP] Ribeiro, Sabrina Marcela [UNESP] Salamanca, Elkin Jahir Florez [UNESP] Da Silva Colin, Jaqueline [UNESP] Klein, Marlise I. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Magdalena |
dc.contributor.author.fl_str_mv |
Pedraza, Midian C. Castillo [UNESP] De Oliveira Fratucelli, Erick Dante [UNESP] Ribeiro, Sabrina Marcela [UNESP] Salamanca, Elkin Jahir Florez [UNESP] Da Silva Colin, Jaqueline [UNESP] Klein, Marlise I. [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Compound 1771 Exopolysaccharides Lipoteichoic acids Myricetin Streptococcusmutans |
topic |
Biofilm Compound 1771 Exopolysaccharides Lipoteichoic acids Myricetin Streptococcusmutans |
description |
Dental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 μg/mL), myricetin (500 μg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:23:01Z 2020-12-12T01:23:01Z 2020-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules25092232 Molecules, v. 25, n. 9, 2020. 1420-3049 http://hdl.handle.net/11449/198825 10.3390/molecules25092232 2-s2.0-85084501969 |
url |
http://dx.doi.org/10.3390/molecules25092232 http://hdl.handle.net/11449/198825 |
identifier_str_mv |
Molecules, v. 25, n. 9, 2020. 1420-3049 10.3390/molecules25092232 2-s2.0-85084501969 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965082339246080 |