Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2017.1361412 http://hdl.handle.net/11449/170206 |
Resumo: | Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA–∆lytS and ∆lytT; LTA–∆dltA and ∆dltD; and insoluble exopolysaccharide–ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms. |
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Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilmsBiofilmseDNAexopolysaccharidesextracellular matrixlipoteichoic acidsStreptococcus mutansStreptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA–∆lytS and ∆lytT; LTA–∆dltA and ∆dltD; and insoluble exopolysaccharide–ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Institute of Dental and Craniofacial ResearchDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp)Center for Oral Biology University of RochesterWhistler Center for Carbohydrate Research Purdue UniversityDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp)FAPESP: 2014/05423-0FAPESP: 2014/21355-4CNPq: 29716CNPq: 35803CNPq: 39550National Institute of Dental and Craniofacial Research: DE017425-06Universidade Estadual Paulista (Unesp)University of RochesterPurdue UniversityCastillo Pedraza, Midian C. [UNESP]Novais, Tatiana F. [UNESP]Faustoferri, Roberta C.Quivey, Robert G.Terekhov, AntonHamaker, Bruce R.Klein, Marlise I. [UNESP]2018-12-11T16:49:45Z2018-12-11T16:49:45Z2017-10-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article722-740application/pdfhttp://dx.doi.org/10.1080/08927014.2017.1361412Biofouling, v. 33, n. 9, p. 722-740, 2017.1029-24540892-7014http://hdl.handle.net/11449/17020610.1080/08927014.2017.13614122-s2.0-850299061532-s2.0-85029906153.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2024-01-08T06:27:12Zoai:repositorio.unesp.br:11449/170206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-08T06:27:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
title |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
spellingShingle |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms Castillo Pedraza, Midian C. [UNESP] Biofilms eDNA exopolysaccharides extracellular matrix lipoteichoic acids Streptococcus mutans |
title_short |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
title_full |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
title_fullStr |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
title_full_unstemmed |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
title_sort |
Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms |
author |
Castillo Pedraza, Midian C. [UNESP] |
author_facet |
Castillo Pedraza, Midian C. [UNESP] Novais, Tatiana F. [UNESP] Faustoferri, Roberta C. Quivey, Robert G. Terekhov, Anton Hamaker, Bruce R. Klein, Marlise I. [UNESP] |
author_role |
author |
author2 |
Novais, Tatiana F. [UNESP] Faustoferri, Roberta C. Quivey, Robert G. Terekhov, Anton Hamaker, Bruce R. Klein, Marlise I. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Rochester Purdue University |
dc.contributor.author.fl_str_mv |
Castillo Pedraza, Midian C. [UNESP] Novais, Tatiana F. [UNESP] Faustoferri, Roberta C. Quivey, Robert G. Terekhov, Anton Hamaker, Bruce R. Klein, Marlise I. [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms eDNA exopolysaccharides extracellular matrix lipoteichoic acids Streptococcus mutans |
topic |
Biofilms eDNA exopolysaccharides extracellular matrix lipoteichoic acids Streptococcus mutans |
description |
Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA–∆lytS and ∆lytT; LTA–∆dltA and ∆dltD; and insoluble exopolysaccharide–ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-21 2018-12-11T16:49:45Z 2018-12-11T16:49:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2017.1361412 Biofouling, v. 33, n. 9, p. 722-740, 2017. 1029-2454 0892-7014 http://hdl.handle.net/11449/170206 10.1080/08927014.2017.1361412 2-s2.0-85029906153 2-s2.0-85029906153.pdf |
url |
http://dx.doi.org/10.1080/08927014.2017.1361412 http://hdl.handle.net/11449/170206 |
identifier_str_mv |
Biofouling, v. 33, n. 9, p. 722-740, 2017. 1029-2454 0892-7014 10.1080/08927014.2017.1361412 2-s2.0-85029906153 2-s2.0-85029906153.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
722-740 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790238449336320 |