Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/216250 |
Resumo: | The centromeric region of the eukaryotic chromosomes is composed of repetitive sequences, being represented mainly by tandem repeats commonly known as satellite DNAs. Studies referring to these highly dynamic sequences have demonstrated their important roles in genomes, especially with regard to genomic evolution, chromosomal composition and species differentiation. Despite their high variability, these sequences may show some conservation among some groups, which makes them attractive for genomic, population and evolutionary studies. Studies referring to the group of fish that address the centromeric composition and possible conservation of satDNAs in this region are scarce and still few explored in recent publications. The present work involved the study of a 177 bp satellite DNA (MelSat03- 177) previously isolated from the genome of the Neotropical fish Megaleporinus elongatus (Order Characiformes) which located in the centromeric region of some chromosomes of the species, in order to analyze the possible conservation of this sequence and to know a few more about its distribution and organization in other fish genomes, mainly in Characiformes. Through a database search, homologous sequences to the satellite MelSat03-177 were retrieved from other species of the Order Characiformes, with a relevant percentage of identity, showing signs of conservation of the sequence. The present work provided initial information, but of great importance for the study of satellites DNA, especially for satDNA in centromeric region, being able to promote several studies related to the distribution, organization and conservation of centromeric repetitive sequences present in the fish group. |
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spelling |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes CharaciformesComparative analysis of a shared satellite DNA in Characiformes fish speciesCentromereSatDNA conservedLibrary hypothesisBiologia molecularDNACharaciformesCentrômeroSatDNA conservadoHipótese da bibliotecaThe centromeric region of the eukaryotic chromosomes is composed of repetitive sequences, being represented mainly by tandem repeats commonly known as satellite DNAs. Studies referring to these highly dynamic sequences have demonstrated their important roles in genomes, especially with regard to genomic evolution, chromosomal composition and species differentiation. Despite their high variability, these sequences may show some conservation among some groups, which makes them attractive for genomic, population and evolutionary studies. Studies referring to the group of fish that address the centromeric composition and possible conservation of satDNAs in this region are scarce and still few explored in recent publications. The present work involved the study of a 177 bp satellite DNA (MelSat03- 177) previously isolated from the genome of the Neotropical fish Megaleporinus elongatus (Order Characiformes) which located in the centromeric region of some chromosomes of the species, in order to analyze the possible conservation of this sequence and to know a few more about its distribution and organization in other fish genomes, mainly in Characiformes. Through a database search, homologous sequences to the satellite MelSat03-177 were retrieved from other species of the Order Characiformes, with a relevant percentage of identity, showing signs of conservation of the sequence. The present work provided initial information, but of great importance for the study of satellites DNA, especially for satDNA in centromeric region, being able to promote several studies related to the distribution, organization and conservation of centromeric repetitive sequences present in the fish group.A região centromérica dos cromossomos dos eucariotos é composta por sequências repetitivas, sendo representadas principalmente por elementos repetidos em tandem comumente conhecidos como DNAs satélites. Os estudos referentes a essas sequências altamente dinâmicas têm demonstrado seus papéis importantes nos genomas, especialmente no que concerne à evolução genômica, composição cromossômica e diferenciação de espécies. Apesar de sua elevada variabilidade, essas sequências podem apresentar certa conservação entre alguns grupos, o que as tornam atrativas para estudos genômicos, populacionais e evolutivos. Os estudos referentes ao grupo dos peixes que abordam a composição centromérica e a possível conservação de satDNAs nessa região são escassos e ainda pouco explorados em publicações recentes. O presente trabalho envolveu o estudo de um DNA satélite de 177 pb (MelSat03-177) previamente isolado do genoma do peixe Neotropical Megaleporinus elongatus (Ordem Characiformes), que está localizado na região centromérica de alguns cromossomos da espécie, no intuito de analisar a possível conservação dessa sequência e conhecer um pouco mais sobre a sua distribuição e organização em outros genomas de peixes, principalmente em Characiformes. Por meio de busca em banco de dados, foram recuperadas sequências homólogas ao satélite MelSat03-177 de outras espécies da Ordem Characiformes, com uma porcentagem relevante de identidade, demonstrando prenúncios de conservação da sequência. O presente trabalho trouxe informações iniciais, mas de grande importância para o estudo de DNAs satélites, especialmente para os satDNA localizados em região centromérica, podendo fomentar diversos estudos relacionados à distribuição, organização e conservação de sequências repetitivas centroméricas presentes no grupo de peixes.Não recebi financiamentoUniversidade Estadual Paulista (Unesp)Parise-Maltempi, Patricia Pasquali [UNESP]Crepaldi, CarolinaUniversidade Estadual Paulista (Unesp)Gonçalves, Évelin Mariani2022-02-01T18:39:02Z2022-02-01T18:39:02Z2022-01-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://hdl.handle.net/11449/216250porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-12-29T06:15:51Zoai:repositorio.unesp.br:11449/216250Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-29T06:15:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes Comparative analysis of a shared satellite DNA in Characiformes fish species |
title |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
spellingShingle |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes Gonçalves, Évelin Mariani Centromere SatDNA conserved Library hypothesis Biologia molecular DNA Characiformes Centrômero SatDNA conservado Hipótese da biblioteca |
title_short |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
title_full |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
title_fullStr |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
title_full_unstemmed |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
title_sort |
Análise comparativa de um DNA satélite compartilhado em espécies de peixes Characiformes |
author |
Gonçalves, Évelin Mariani |
author_facet |
Gonçalves, Évelin Mariani |
author_role |
author |
dc.contributor.none.fl_str_mv |
Parise-Maltempi, Patricia Pasquali [UNESP] Crepaldi, Carolina Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gonçalves, Évelin Mariani |
dc.subject.por.fl_str_mv |
Centromere SatDNA conserved Library hypothesis Biologia molecular DNA Characiformes Centrômero SatDNA conservado Hipótese da biblioteca |
topic |
Centromere SatDNA conserved Library hypothesis Biologia molecular DNA Characiformes Centrômero SatDNA conservado Hipótese da biblioteca |
description |
The centromeric region of the eukaryotic chromosomes is composed of repetitive sequences, being represented mainly by tandem repeats commonly known as satellite DNAs. Studies referring to these highly dynamic sequences have demonstrated their important roles in genomes, especially with regard to genomic evolution, chromosomal composition and species differentiation. Despite their high variability, these sequences may show some conservation among some groups, which makes them attractive for genomic, population and evolutionary studies. Studies referring to the group of fish that address the centromeric composition and possible conservation of satDNAs in this region are scarce and still few explored in recent publications. The present work involved the study of a 177 bp satellite DNA (MelSat03- 177) previously isolated from the genome of the Neotropical fish Megaleporinus elongatus (Order Characiformes) which located in the centromeric region of some chromosomes of the species, in order to analyze the possible conservation of this sequence and to know a few more about its distribution and organization in other fish genomes, mainly in Characiformes. Through a database search, homologous sequences to the satellite MelSat03-177 were retrieved from other species of the Order Characiformes, with a relevant percentage of identity, showing signs of conservation of the sequence. The present work provided initial information, but of great importance for the study of satellites DNA, especially for satDNA in centromeric region, being able to promote several studies related to the distribution, organization and conservation of centromeric repetitive sequences present in the fish group. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-01T18:39:02Z 2022-02-01T18:39:02Z 2022-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11449/216250 |
url |
http://hdl.handle.net/11449/216250 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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