Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-019-05403-8 http://hdl.handle.net/11449/189206 |
Resumo: | Endocrine disrupting chemicals are one of the most important factors contributing to worldwide amphibian decline. The 4-nonylphenol (NP) is a degradation product of several compounds, such as detergents and pesticides, affecting the aquatic environment. Here, we test whether treatment with NP has an effect on developing ovarian tissue, nuclear abnormalities in erythrocytes, and body darkness in pre-metamorphic tadpoles of the bullfrog Lithobates catesbeianus. Tadpoles were exposed for 14 days to three different concentrations of NP (1, 10, and 100 μg/L) besides the control group, which was maintained only with water. After determining body coloration, animals were euthanized and gonads and blood were collected and processed for histology and genotoxic analysis. Even though most animals were females, intersex tadpoles were observed in control and treated groups and there were no males in any group. The highest concentration of NP showed an increase in atretic oocytes, but the area corresponding to somatic compartment and early and late germ cells were not affected. Furthermore, all treated groups presented higher amount of nuclear abnormalities in erythrocytes and body darkening when compared with the control group. These results suggest that NP causes genetic damage and morphological alterations in L. catesbeianus tadpoles by disrupting oogenesis, inducing genotoxicity and increasing body coloration. Its effects on gonadal development could cause future impairments in reproduction, while its deleterious effects on genotoxicity and body pigmentation could be used as a biomarker of effect to this compound. |
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Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenolBody pigmentationCellular abnormalitiesEndocrine disrupting chemicalsEstrogenic compoundsGonadal differentiationOogenesisEndocrine disrupting chemicals are one of the most important factors contributing to worldwide amphibian decline. The 4-nonylphenol (NP) is a degradation product of several compounds, such as detergents and pesticides, affecting the aquatic environment. Here, we test whether treatment with NP has an effect on developing ovarian tissue, nuclear abnormalities in erythrocytes, and body darkness in pre-metamorphic tadpoles of the bullfrog Lithobates catesbeianus. Tadpoles were exposed for 14 days to three different concentrations of NP (1, 10, and 100 μg/L) besides the control group, which was maintained only with water. After determining body coloration, animals were euthanized and gonads and blood were collected and processed for histology and genotoxic analysis. Even though most animals were females, intersex tadpoles were observed in control and treated groups and there were no males in any group. The highest concentration of NP showed an increase in atretic oocytes, but the area corresponding to somatic compartment and early and late germ cells were not affected. Furthermore, all treated groups presented higher amount of nuclear abnormalities in erythrocytes and body darkening when compared with the control group. These results suggest that NP causes genetic damage and morphological alterations in L. catesbeianus tadpoles by disrupting oogenesis, inducing genotoxicity and increasing body coloration. Its effects on gonadal development could cause future impairments in reproduction, while its deleterious effects on genotoxicity and body pigmentation could be used as a biomarker of effect to this compound.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Post-graduate Program in Animal Biology Department of Biology São Paulo State University (UNESP)Instituto de Biodiversidad y Biología Experimental y Aplicada-CONICETLaboratorio de Neuroendocrinología y Comportamiento Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas - UNESP/IBILCE, Rua Cristóvão Colombo, 2265, Bairro: Jardim NazarethInstituto de Biociências (InBio) Universidade Federal de Mato Grosso do Sul UFMSPost-graduate Program in Animal Biology Department of Biology São Paulo State University (UNESP)Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas - UNESP/IBILCE, Rua Cristóvão Colombo, 2265, Bairro: Jardim NazarethFAPESP: 2014/00946-4FAPESP: 2015/12006-9CNPq: 305081/2015-2Universidade Estadual Paulista (Unesp)Instituto de Biodiversidad y Biología Experimental y Aplicada-CONICETUniversidad de Buenos AiresUniversidade Federal de Mato Grosso do Sul (UFMS)Scaia, María Florencia [UNESP]de Gregorio, Lara Salgueiro [UNESP]Franco-Belussi, Lilian [UNESP]Succi-Domingues, Maysa [UNESP]de Oliveira, Classius [UNESP]2019-10-06T16:33:25Z2019-10-06T16:33:25Z2019-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22209-22219http://dx.doi.org/10.1007/s11356-019-05403-8Environmental Science and Pollution Research, v. 26, n. 22, p. 22209-22219, 2019.1614-74990944-1344http://hdl.handle.net/11449/18920610.1007/s11356-019-05403-82-s2.0-85066783752Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2021-10-23T14:48:05Zoai:repositorio.unesp.br:11449/189206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:48:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
title |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
spellingShingle |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol Scaia, María Florencia [UNESP] Body pigmentation Cellular abnormalities Endocrine disrupting chemicals Estrogenic compounds Gonadal differentiation Oogenesis |
title_short |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
title_full |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
title_fullStr |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
title_full_unstemmed |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
title_sort |
Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol |
author |
Scaia, María Florencia [UNESP] |
author_facet |
Scaia, María Florencia [UNESP] de Gregorio, Lara Salgueiro [UNESP] Franco-Belussi, Lilian [UNESP] Succi-Domingues, Maysa [UNESP] de Oliveira, Classius [UNESP] |
author_role |
author |
author2 |
de Gregorio, Lara Salgueiro [UNESP] Franco-Belussi, Lilian [UNESP] Succi-Domingues, Maysa [UNESP] de Oliveira, Classius [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Biodiversidad y Biología Experimental y Aplicada-CONICET Universidad de Buenos Aires Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Scaia, María Florencia [UNESP] de Gregorio, Lara Salgueiro [UNESP] Franco-Belussi, Lilian [UNESP] Succi-Domingues, Maysa [UNESP] de Oliveira, Classius [UNESP] |
dc.subject.por.fl_str_mv |
Body pigmentation Cellular abnormalities Endocrine disrupting chemicals Estrogenic compounds Gonadal differentiation Oogenesis |
topic |
Body pigmentation Cellular abnormalities Endocrine disrupting chemicals Estrogenic compounds Gonadal differentiation Oogenesis |
description |
Endocrine disrupting chemicals are one of the most important factors contributing to worldwide amphibian decline. The 4-nonylphenol (NP) is a degradation product of several compounds, such as detergents and pesticides, affecting the aquatic environment. Here, we test whether treatment with NP has an effect on developing ovarian tissue, nuclear abnormalities in erythrocytes, and body darkness in pre-metamorphic tadpoles of the bullfrog Lithobates catesbeianus. Tadpoles were exposed for 14 days to three different concentrations of NP (1, 10, and 100 μg/L) besides the control group, which was maintained only with water. After determining body coloration, animals were euthanized and gonads and blood were collected and processed for histology and genotoxic analysis. Even though most animals were females, intersex tadpoles were observed in control and treated groups and there were no males in any group. The highest concentration of NP showed an increase in atretic oocytes, but the area corresponding to somatic compartment and early and late germ cells were not affected. Furthermore, all treated groups presented higher amount of nuclear abnormalities in erythrocytes and body darkening when compared with the control group. These results suggest that NP causes genetic damage and morphological alterations in L. catesbeianus tadpoles by disrupting oogenesis, inducing genotoxicity and increasing body coloration. Its effects on gonadal development could cause future impairments in reproduction, while its deleterious effects on genotoxicity and body pigmentation could be used as a biomarker of effect to this compound. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:33:25Z 2019-10-06T16:33:25Z 2019-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-019-05403-8 Environmental Science and Pollution Research, v. 26, n. 22, p. 22209-22219, 2019. 1614-7499 0944-1344 http://hdl.handle.net/11449/189206 10.1007/s11356-019-05403-8 2-s2.0-85066783752 |
url |
http://dx.doi.org/10.1007/s11356-019-05403-8 http://hdl.handle.net/11449/189206 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 26, n. 22, p. 22209-22219, 2019. 1614-7499 0944-1344 10.1007/s11356-019-05403-8 2-s2.0-85066783752 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22209-22219 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964641719222272 |