Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12936-016-1414-3 http://hdl.handle.net/11449/161729 |
Resumo: | Background: The immune response against Plasmodium vivax immunogenic epitopes is regulated by pro-and anti-inflammatory cytokines that determine antibody levels and class switching. Cytokine gene polymorphisms may be responsible for changes in the humoral immune response against malaria. The aim of this study was to evaluate whether polymorphisms in the TNFA, IFNG and IL10 genes would alter the levels of anti-PvAMA1, PvDBP and -PvMSP-1(19) IgG antibodies in patients with vivax malaria. Methods: Samples from 90 vivax malaria-infected and 51 uninfected subjects from an endemic area of the Brazilian Amazon were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify polymorphisms of the genes TNFA (-1031T > C, -308G > A, -238G > A), IFNG (+874T > A) and IL10 (-819C > T, -592C > A). The levels of total IgG against PvAMA1, PvDBP and -PvMSP-1(19) were determined using an enzyme-linked immunosorbent assay (ELISA). Associations between the polymorphisms and the antibody response were assessed by means of logistic regression models. Results: No significant differences were found in the levels of IgG antibodies against the PvAMA-1, PvDBP or PvMSP-1(19) proteins in relation to the studied polymorphisms. Conclusions: Although no associations were found among the evaluated genotypes and alleles and anti-merozoite IgG class P. vivax antibody levels, this study helps elucidate the immunogenic profile involved in the humoral immune response in malaria. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian AmazonIgG antibodyPlasmodium vivaxTNFIFNGIL10Background: The immune response against Plasmodium vivax immunogenic epitopes is regulated by pro-and anti-inflammatory cytokines that determine antibody levels and class switching. Cytokine gene polymorphisms may be responsible for changes in the humoral immune response against malaria. The aim of this study was to evaluate whether polymorphisms in the TNFA, IFNG and IL10 genes would alter the levels of anti-PvAMA1, PvDBP and -PvMSP-1(19) IgG antibodies in patients with vivax malaria. Methods: Samples from 90 vivax malaria-infected and 51 uninfected subjects from an endemic area of the Brazilian Amazon were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify polymorphisms of the genes TNFA (-1031T > C, -308G > A, -238G > A), IFNG (+874T > A) and IL10 (-819C > T, -592C > A). The levels of total IgG against PvAMA1, PvDBP and -PvMSP-1(19) were determined using an enzyme-linked immunosorbent assay (ELISA). Associations between the polymorphisms and the antibody response were assessed by means of logistic regression models. Results: No significant differences were found in the levels of IgG antibodies against the PvAMA-1, PvDBP or PvMSP-1(19) proteins in relation to the studied polymorphisms. Conclusions: Although no associations were found among the evaluated genotypes and alleles and anti-merozoite IgG class P. vivax antibody levels, this study helps elucidate the immunogenic profile involved in the humoral immune response in malaria.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Para State Research Support Foundation (Fundacao de Amparo a Pesquisa do Estado do Para)CAPPESCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Jose Rio Preto Med Sch, Dept Dermatol Infect & Parasit Dis, Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilUniv Fed Sergipe, Dept Biol, Lab Mol Genet & Biotechnol, Sao Cristovao, SE, BrazilUniv Fed Para, UFPA, Inst Biol Sci, Lab Microbiol & Immunol, Belem, Para, BrazilUniv Estadual Campinas, Lab Trop Dis, Dept Genet Evolut & Bioagents, Campinas, SP, BrazilEvandro Chagas Inst, Sect Parasitol, Lab Basic Res Malaria, Belem, PA, BrazilSao Paulo State Univ, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilCNPq: 472135/2012Biomed Central LtdUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Federal de Sergipe (UFS)Universidade Federal do Pará (UFPA)Universidade Estadual de Campinas (UNICAMP)Evandro Chagas InstFurini, Adriana A. C.Capobianco, Marcela P. [UNESP]Storti-Melo, Luciane M.Cunha, Maristela G.Cassiano, Gustavo C.Machado, Ricardo Luiz D. [UNESP]2018-11-26T16:48:24Z2018-11-26T16:48:24Z2016-07-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/s12936-016-1414-3Malaria Journal. London: Biomed Central Ltd, v. 15, 8 p., 2016.1475-2875http://hdl.handle.net/11449/16172910.1186/s12936-016-1414-3WOS:000379813700006WOS000379813700006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMalaria Journal2,082info:eu-repo/semantics/openAccess2023-10-20T06:05:17Zoai:repositorio.unesp.br:11449/161729Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-20T06:05:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
title |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
spellingShingle |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon Furini, Adriana A. C. IgG antibody Plasmodium vivax TNF IFNG IL10 |
title_short |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
title_full |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
title_fullStr |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
title_full_unstemmed |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
title_sort |
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-1(19) IgG antibody levels in a malaria-endemic area of the Brazilian Amazon |
author |
Furini, Adriana A. C. |
author_facet |
Furini, Adriana A. C. Capobianco, Marcela P. [UNESP] Storti-Melo, Luciane M. Cunha, Maristela G. Cassiano, Gustavo C. Machado, Ricardo Luiz D. [UNESP] |
author_role |
author |
author2 |
Capobianco, Marcela P. [UNESP] Storti-Melo, Luciane M. Cunha, Maristela G. Cassiano, Gustavo C. Machado, Ricardo Luiz D. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Federal de Sergipe (UFS) Universidade Federal do Pará (UFPA) Universidade Estadual de Campinas (UNICAMP) Evandro Chagas Inst |
dc.contributor.author.fl_str_mv |
Furini, Adriana A. C. Capobianco, Marcela P. [UNESP] Storti-Melo, Luciane M. Cunha, Maristela G. Cassiano, Gustavo C. Machado, Ricardo Luiz D. [UNESP] |
dc.subject.por.fl_str_mv |
IgG antibody Plasmodium vivax TNF IFNG IL10 |
topic |
IgG antibody Plasmodium vivax TNF IFNG IL10 |
description |
Background: The immune response against Plasmodium vivax immunogenic epitopes is regulated by pro-and anti-inflammatory cytokines that determine antibody levels and class switching. Cytokine gene polymorphisms may be responsible for changes in the humoral immune response against malaria. The aim of this study was to evaluate whether polymorphisms in the TNFA, IFNG and IL10 genes would alter the levels of anti-PvAMA1, PvDBP and -PvMSP-1(19) IgG antibodies in patients with vivax malaria. Methods: Samples from 90 vivax malaria-infected and 51 uninfected subjects from an endemic area of the Brazilian Amazon were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify polymorphisms of the genes TNFA (-1031T > C, -308G > A, -238G > A), IFNG (+874T > A) and IL10 (-819C > T, -592C > A). The levels of total IgG against PvAMA1, PvDBP and -PvMSP-1(19) were determined using an enzyme-linked immunosorbent assay (ELISA). Associations between the polymorphisms and the antibody response were assessed by means of logistic regression models. Results: No significant differences were found in the levels of IgG antibodies against the PvAMA-1, PvDBP or PvMSP-1(19) proteins in relation to the studied polymorphisms. Conclusions: Although no associations were found among the evaluated genotypes and alleles and anti-merozoite IgG class P. vivax antibody levels, this study helps elucidate the immunogenic profile involved in the humoral immune response in malaria. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-19 2018-11-26T16:48:24Z 2018-11-26T16:48:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12936-016-1414-3 Malaria Journal. London: Biomed Central Ltd, v. 15, 8 p., 2016. 1475-2875 http://hdl.handle.net/11449/161729 10.1186/s12936-016-1414-3 WOS:000379813700006 WOS000379813700006.pdf |
url |
http://dx.doi.org/10.1186/s12936-016-1414-3 http://hdl.handle.net/11449/161729 |
identifier_str_mv |
Malaria Journal. London: Biomed Central Ltd, v. 15, 8 p., 2016. 1475-2875 10.1186/s12936-016-1414-3 WOS:000379813700006 WOS000379813700006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Malaria Journal 2,082 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964629312471040 |