Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6882-13-208 http://hdl.handle.net/11449/76274 |
Resumo: | Background: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1β in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL).Conclusions: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic. © 2013 de Oliveira et al.; licensee BioMed Central Ltd. |
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Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistryCandida albicansCandida glabrataCandida tropicalisEquisetum arvense LGlycyrrhiza glabra LPunica granatum LStaphylococcus aureusStaphylococcus epidermidisStreptococcus mutansStryphnodendron barbatimam MartBrazilian plant extractequisetum arvense extractGlycyrrhiza glabra extractinterleukin 1betaplant extractpomegranate extractStryphnodendron barbatimam extracttumor necrosis factor alphaunclassified druganimal cellantimicrobial activitycell viabilitycolorimetrycontrolled studycytokine productioncytotoxicitydentistryenzyme linked immunosorbent assaymacrophagemousemouth floranonhumanstimulationAnimalsAnti-Bacterial AgentsBacteriaBrazilCell LineCell SurvivalMacrophagesMicePlant ExtractsPlants, MedicinalStomatognathic DiseasesTumor Necrosis Factor-alphaBackground: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1β in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL).Conclusions: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic. © 2013 de Oliveira et al.; licensee BioMed Central Ltd.Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology Institute of Science and Technology Univ Estadual Paulista/UNESP, Av. Engenheiro Francisco Jose Longo 777 - Jardim Sao Dimas, São José dos Campos, SPDepartment of Biosciences and Oral Diagnosis, Laboratory of Biochemistry and Pharmacology Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SPDepartment of Restorative Dentistry Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SPDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology Institute of Science and Technology Univ Estadual Paulista/UNESP, Av. Engenheiro Francisco Jose Longo 777 - Jardim Sao Dimas, São José dos Campos, SPDepartment of Biosciences and Oral Diagnosis, Laboratory of Biochemistry and Pharmacology Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SPDepartment of Restorative Dentistry Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SPUniversidade Estadual Paulista (Unesp)de Oliveira, Jonatas Rafael [UNESP]de Castro, Vinicius Carlos [UNESP]Vilela, Polyana das Graças Figueiredo [UNESP]Camargo, Samira Esteves Afonso [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]de Oliveira, Luciane Dias [UNESP]2014-05-27T11:30:09Z2014-05-27T11:30:09Z2013-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1472-6882-13-208BMC Complementary and Alternative Medicine, v. 13.1472-6882http://hdl.handle.net/11449/7627410.1186/1472-6882-13-208WOS:0003232286000012-s2.0-848825866292-s2.0-84882586629.pdf0053567153623569471667661133031193046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2023-12-06T06:18:00Zoai:repositorio.unesp.br:11449/76274Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-06T06:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
title |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
spellingShingle |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry de Oliveira, Jonatas Rafael [UNESP] Candida albicans Candida glabrata Candida tropicalis Equisetum arvense L Glycyrrhiza glabra L Punica granatum L Staphylococcus aureus Staphylococcus epidermidis Streptococcus mutans Stryphnodendron barbatimam Mart Brazilian plant extract equisetum arvense extract Glycyrrhiza glabra extract interleukin 1beta plant extract pomegranate extract Stryphnodendron barbatimam extract tumor necrosis factor alpha unclassified drug animal cell antimicrobial activity cell viability colorimetry controlled study cytokine production cytotoxicity dentistry enzyme linked immunosorbent assay macrophage mouse mouth flora nonhuman stimulation Animals Anti-Bacterial Agents Bacteria Brazil Cell Line Cell Survival Macrophages Mice Plant Extracts Plants, Medicinal Stomatognathic Diseases Tumor Necrosis Factor-alpha |
title_short |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
title_full |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
title_fullStr |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
title_full_unstemmed |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
title_sort |
Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry |
author |
de Oliveira, Jonatas Rafael [UNESP] |
author_facet |
de Oliveira, Jonatas Rafael [UNESP] de Castro, Vinicius Carlos [UNESP] Vilela, Polyana das Graças Figueiredo [UNESP] Camargo, Samira Esteves Afonso [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
de Castro, Vinicius Carlos [UNESP] Vilela, Polyana das Graças Figueiredo [UNESP] Camargo, Samira Esteves Afonso [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Oliveira, Jonatas Rafael [UNESP] de Castro, Vinicius Carlos [UNESP] Vilela, Polyana das Graças Figueiredo [UNESP] Camargo, Samira Esteves Afonso [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans Candida glabrata Candida tropicalis Equisetum arvense L Glycyrrhiza glabra L Punica granatum L Staphylococcus aureus Staphylococcus epidermidis Streptococcus mutans Stryphnodendron barbatimam Mart Brazilian plant extract equisetum arvense extract Glycyrrhiza glabra extract interleukin 1beta plant extract pomegranate extract Stryphnodendron barbatimam extract tumor necrosis factor alpha unclassified drug animal cell antimicrobial activity cell viability colorimetry controlled study cytokine production cytotoxicity dentistry enzyme linked immunosorbent assay macrophage mouse mouth flora nonhuman stimulation Animals Anti-Bacterial Agents Bacteria Brazil Cell Line Cell Survival Macrophages Mice Plant Extracts Plants, Medicinal Stomatognathic Diseases Tumor Necrosis Factor-alpha |
topic |
Candida albicans Candida glabrata Candida tropicalis Equisetum arvense L Glycyrrhiza glabra L Punica granatum L Staphylococcus aureus Staphylococcus epidermidis Streptococcus mutans Stryphnodendron barbatimam Mart Brazilian plant extract equisetum arvense extract Glycyrrhiza glabra extract interleukin 1beta plant extract pomegranate extract Stryphnodendron barbatimam extract tumor necrosis factor alpha unclassified drug animal cell antimicrobial activity cell viability colorimetry controlled study cytokine production cytotoxicity dentistry enzyme linked immunosorbent assay macrophage mouse mouth flora nonhuman stimulation Animals Anti-Bacterial Agents Bacteria Brazil Cell Line Cell Survival Macrophages Mice Plant Extracts Plants, Medicinal Stomatognathic Diseases Tumor Necrosis Factor-alpha |
description |
Background: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1β in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL).Conclusions: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic. © 2013 de Oliveira et al.; licensee BioMed Central Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-15 2014-05-27T11:30:09Z 2014-05-27T11:30:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6882-13-208 BMC Complementary and Alternative Medicine, v. 13. 1472-6882 http://hdl.handle.net/11449/76274 10.1186/1472-6882-13-208 WOS:000323228600001 2-s2.0-84882586629 2-s2.0-84882586629.pdf 0053567153623569 4716676611330311 9304600166583100 0000-0003-0987-5594 |
url |
http://dx.doi.org/10.1186/1472-6882-13-208 http://hdl.handle.net/11449/76274 |
identifier_str_mv |
BMC Complementary and Alternative Medicine, v. 13. 1472-6882 10.1186/1472-6882-13-208 WOS:000323228600001 2-s2.0-84882586629 2-s2.0-84882586629.pdf 0053567153623569 4716676611330311 9304600166583100 0000-0003-0987-5594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 2.109 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789921695498240 |