Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-9946202163054 http://hdl.handle.net/11449/212531 |
Resumo: | Many infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks. |
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Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus speciesNeonatal milkHuman milk banksBreastfeedingMultidrug-resistant bacteriaStaphylococcal toxinsMany infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Estadual Paulista, Faculdade de MedicinaUniversidade de Feira de Santana, Departamento de Ciências BiológicasUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Estadual Paulista, Faculdade de MedicinaFAPESP: 2010/50823-5FAPESP: 2008/53499-4CNPq: PQ-1DInstituto de Medicina Tropical de São PauloUniversidade Estadual Paulista (Unesp)Universidade de Feira de SantanaSalerno, Tatiana [UNESP]Siqueira, Amanda Keller [UNESP]Pinto, José Paes De Almeida Nogueira [UNESP]Cunha, Maria De Lourdes Ribeiro De Souza Da [UNESP]Silvestre, Patrícia Kelly [UNESP]Condas, Larissa Anuska Zeni [UNESP]Lara, Gustavo Henrique Batista [UNESP]Pereira, Juliano Gonçalves [UNESP]Silva, Aristeu Vieira DaListoni, Fernando José Paganini [UNESP]Martins, Lorrayne De Souza Araújo [UNESP]Motta, Rodrigo Garcia [UNESP]Ribeiro, Márcio Garcia [UNESP]2021-07-14T10:41:24Z2021-07-14T10:41:24Z2021-07-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/S1678-9946202163054Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical de São Paulo, v. 63, p. -, 2021.1678-9946http://hdl.handle.net/11449/21253110.1590/S1678-9946202163054S0036-46652021000100229S0036-46652021000100229.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccess2023-10-25T06:07:25Zoai:repositorio.unesp.br:11449/212531Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-25T06:07:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
spellingShingle |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species Salerno, Tatiana [UNESP] Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
title_short |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_full |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_fullStr |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_full_unstemmed |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_sort |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
author |
Salerno, Tatiana [UNESP] |
author_facet |
Salerno, Tatiana [UNESP] Siqueira, Amanda Keller [UNESP] Pinto, José Paes De Almeida Nogueira [UNESP] Cunha, Maria De Lourdes Ribeiro De Souza Da [UNESP] Silvestre, Patrícia Kelly [UNESP] Condas, Larissa Anuska Zeni [UNESP] Lara, Gustavo Henrique Batista [UNESP] Pereira, Juliano Gonçalves [UNESP] Silva, Aristeu Vieira Da Listoni, Fernando José Paganini [UNESP] Martins, Lorrayne De Souza Araújo [UNESP] Motta, Rodrigo Garcia [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author_role |
author |
author2 |
Siqueira, Amanda Keller [UNESP] Pinto, José Paes De Almeida Nogueira [UNESP] Cunha, Maria De Lourdes Ribeiro De Souza Da [UNESP] Silvestre, Patrícia Kelly [UNESP] Condas, Larissa Anuska Zeni [UNESP] Lara, Gustavo Henrique Batista [UNESP] Pereira, Juliano Gonçalves [UNESP] Silva, Aristeu Vieira Da Listoni, Fernando José Paganini [UNESP] Martins, Lorrayne De Souza Araújo [UNESP] Motta, Rodrigo Garcia [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Feira de Santana |
dc.contributor.author.fl_str_mv |
Salerno, Tatiana [UNESP] Siqueira, Amanda Keller [UNESP] Pinto, José Paes De Almeida Nogueira [UNESP] Cunha, Maria De Lourdes Ribeiro De Souza Da [UNESP] Silvestre, Patrícia Kelly [UNESP] Condas, Larissa Anuska Zeni [UNESP] Lara, Gustavo Henrique Batista [UNESP] Pereira, Juliano Gonçalves [UNESP] Silva, Aristeu Vieira Da Listoni, Fernando José Paganini [UNESP] Martins, Lorrayne De Souza Araújo [UNESP] Motta, Rodrigo Garcia [UNESP] Ribeiro, Márcio Garcia [UNESP] |
dc.subject.por.fl_str_mv |
Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
topic |
Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
description |
Many infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-14T10:41:24Z 2021-07-14T10:41:24Z 2021-07-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-9946202163054 Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical de São Paulo, v. 63, p. -, 2021. 1678-9946 http://hdl.handle.net/11449/212531 10.1590/S1678-9946202163054 S0036-46652021000100229 S0036-46652021000100229.pdf |
url |
http://dx.doi.org/10.1590/S1678-9946202163054 http://hdl.handle.net/11449/212531 |
identifier_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical de São Paulo, v. 63, p. -, 2021. 1678-9946 10.1590/S1678-9946202163054 S0036-46652021000100229 S0036-46652021000100229.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789503718424576 |